The relationship between reactive oxygen species, DNA fragmentation, and sperm parameters in human sperm using simplified sucrose vitrification with or without triple antioxidant supplementation

Juanpanich, Theesit; Suttirojpattana, Tayita; Parnpai, Rangsun; Vutyavanich, Teraporn

doi:10.5653/cerm.2021.05120

Cited by 3 publications

(2 citation statements)

References 53 publications

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“…Last, we utilized frozen sperm samples for DNA extraction. We acknowledge that frozen sperm may exhibit lower DNA integrity, viability, and motility values compared to fresh sperm [ 74 , 75 ]; however, it is challenging to preserve and manage fresh sperm over the long term; and there is limited information available regarding the effects of cryopreservation on epigenetic modulation in sperm, and existing studies present contradictory results [ 75 ]. In the future studies, we intend to investigate the impact of the cryopreservation process on sperm DNA methylation.…”

Section: Discussionmentioning

confidence: 99%

DNA methylation patterns in patients with asthenospermia and oligoasthenospermia

Zhang,

Li,

Wang

et al. 2024

BMC Genomics

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Background Spermatogenesis is a highly regulated and complex process in which DNA methylation plays a crucial role. This study aimed to explore the differential methylation profiles in sperm DNA between patients with asthenospermia (AS) and healthy controls (HCs), those with oligoasthenospermia (OAS) and HCs, and patients with AS and those with OAS. Results Semen samples and clinical data were collected from five patients with AS, five patients with OAS, and six age-matched HCs. Reduced representation bisulfite sequencing (RRBS) was performed to identify differentially methylated regions (DMRs) in sperm cells among the different types of patients and HCs. A total of 6520, 28,019, and 16,432 DMRs were detected between AS and HC, OAS and HC, and AS and OAS groups, respectively. These DMRs were predominantly located within gene bodies and mapped to 2868, 9296, and 9090 genes in the respective groups. Of note, 12, 9, and 8 DMRs in each group were closely associated with spermatogenesis and male infertility. Furthermore, BDNF, SMARCB1, PIK3CA, and DDX27; RBMX and SPATA17; ASZ1, CDH1, and CHDH were identified as strong differentially methylated candidate genes in each group, respectively. Meanwhile, the GO analysis of DMR-associated genes in the AS vs. HC groups revealed that protein binding, cytoplasm, and transcription (DNA-templated) were the most enriched terms in the biological process (BP), cellular component (CC), and molecular function (MF), respectively. Likewise, in both the OAS vs. HC and AS vs. OAS groups, GO analysis revealed protein binding, nucleus, and transcription (DNA-templated) as the most enriched terms in BP, CC, and MF, respectively. Finally, the KEGG analysis of DMR-annotated genes and these genes at promoters suggested that metabolic pathways were the most significantly associated across all three groups. Conclusions The current study results revealed distinctive sperm DNA methylation patterns in the AS vs. HC and OAS vs. HC groups, particularly between patients with AS and those with OAS. The identification of key genes associated with spermatogenesis and male infertility in addition to the differentially enriched metabolic pathways may contribute to uncovering the potential pathogenesis in different types of abnormal sperm parameters.

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Section: Discussionmentioning

confidence: 99%

DNA methylation patterns in patients with asthenospermia and oligoasthenospermia

Zhang,

Li,

Wang

et al. 2024

BMC Genomics

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“…El botón espermático se resuspendió en 1 mL de PBS antes de cuantificar por citometría de flujo en un FACSCalibur (Becton Dickinson). Se utilizó una longitud de onda de excitación de 488 nm y longitud de onda de emisión de 520 nm; se contaron, al menos 10,000 eventos por muestra y se determinó la media de fluorescencia [90,91] utilizando el sistema de cómputo Flowing Software, versión 2.5.1.…”

Section: Materials Y Métodosunclassified

Efecto del uso de la C-Ficocianina, como agente antioxidante, durante el almacenamiento de espermatozoide de cerdo (Sus scrofa), en un diluyente de corta duración, a una temperatura de 16 °C

Berber Arand

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Iztapalapa (UAM-I), manifiesto mi compromiso de mantener de forma confidencial y de no utilizar, divulgar o difundir por ningún medio, en beneficio propio o de terceros, la información, la documentación y los datos de toda índole a los que tenga acceso y reciba con motivo del proyecto de investigación "Efecto del uso de la C-Ficocianina, como agente antioxidante, durante el almacenamiento de espermatozoide de cerdo (Sus scrofa), en un diluyente de corta duración, a una temperatura de 16°C" a desarrollar en la Universidad Autónoma Metropolitana, Iztapalapa. Lo anterior, en términos del artículo 6, fracción V, de

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Carboxylated ε-Poly-l-lysine Improves Post-Thaw Quality, Mitochondrial Functions and Antioxidant Defense of Goat Cryopreserved Sperm

Zhang

Cui

Ding

et al. 2023

Biology

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Carboxylated ε-poly-l-lysine (CPLL), a novel cryoprotectant, can protect the sperm membranes by inhibiting ice crystal formation during the cryopreservation process. The present study was conducted to investigate the consequence of CPLL supplementation on the post-thaw quality of cryopreserved goat sperm. For this, different doses (0, 0.5%, 1%, 1.5%, and 2%; v/v) of CPLL were added to the cryopreservation medium, and the motility, membrane and acrosome integrity, mitochondrial membrane potential (MMP), ATP level, ROS production, anti-oxidant defense system, malondialdehyde (MDA) level, and apoptosis in post-thaw sperm were evaluated. It was observed that the addition of 1% CPLL significantly (p < 0.05) increased the total motility, membrane integrity, acrosome integrity, and catalase (CAT) activity of post-thaw sperm compared to those of control and other CPLL doses. The ATP content was observed significantly (p < 0.05) higher in 0.5% and 1% CPLL, however, the SOD activity and progressive motility were significantly (p < 0.05) increased by adding CPLL at 1% and 1.5% level. Moreover, the addition of CPLL at 1% dose not only showed a lower percentage of apoptosis, but also significantly (p < 0.05) increased the MMP while reducing ROS production and MDA levels compared to those of other CPLL doses and/or control. Therefore, it is clear that the supplementation of 1% CPLL can remarkably improve the post-thaw goat sperm motility, membrane and acrosome integrity, antioxidant abundance, mitochondrial potentials, and ATP supply by protecting the sperm from cryodamage and undergoing apoptosis. These findings will provide novel insights into sperm cryobiology.

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The relationship between reactive oxygen species, DNA fragmentation, and sperm parameters in human sperm using simplified sucrose vitrification with or without triple antioxidant supplementation

Cited by 3 publications

References 53 publications

DNA methylation patterns in patients with asthenospermia and oligoasthenospermia

DNA methylation patterns in patients with asthenospermia and oligoasthenospermia

Efecto del uso de la C-Ficocianina, como agente antioxidante, durante el almacenamiento de espermatozoide de cerdo (Sus scrofa), en un diluyente de corta duración, a una temperatura de 16 °C

Carboxylated ε-Poly-l-lysine Improves Post-Thaw Quality, Mitochondrial Functions and Antioxidant Defense of Goat Cryopreserved Sperm

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