The future of education relies on the integration of information technologies, emphasizing the importance of equity and inclusiveness for quality education. Teacher education programs are essential for fostering qualified educators for the future. Integrating AI in education is crucial to ensure inclusivity and comprehensive services for all. This study aims to evaluate student teachers’ perceptions of using AI in learning and teaching, and to provide suggestions for enhancing sustainable education through information technologies. A qualitative research design was adopted to gather perceptions and experiences from 240 student teachers who participated in a seminar on AI usage and completed self-reflection tasks. These student teachers, enrolled in various teaching methods and principal courses, contributed to the thematic analysis. The study reveals that AI should be carefully planned and incorporated into lesson plans to enhance personalized learning. Student teachers reported that AI supports and motivates the learning process, effectively transforming students’ needs and learning experiences. However, they also noted potential drawbacks, such as AI imposing restrictions on the teaching profession, replacing teachers, and producing biased results. The study suggests that capacity-building strategies for student teachers should be enriched across different courses to raise awareness about AI’s applications.