The Relationship Between Serum TARC, TSLP and POSTN Levels and Childhood Atopic Dermatitis

Uysal, Pınar; Birtekocak, Fatih; Karul, Aslıhan

doi:10.7754/clin.lab.2017.161107

Cited by 15 publications

(20 citation statements)

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“…Kou et al [20] detected higher levels of serum periostin in patients with erythrodermic type AD and widespread type AD, as compared to patients with lesions that had not spread systemically. Uysal et al reported significant correlations between plasma periostin levels and the duration of symptoms and the presence of atopy [21]. Our results contrast with these study results, and this difference may be associated with sample sizes, recruitment strategies and ELISA kit-specific changes.…”

Section: Discussioncontrasting

confidence: 99%

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Evaluation of periostin level for predicting severity and chronicity of childhood atopic dermatitis

Özçeker

Yücel

Sipahi

et al. 2019

pdia

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IntroductionPeriostin has some effects on the pathogenesis of atopic dermatitis (AD) via release of pro-inflammatory cytokines and chemokines from activated keratinocytes and it is related to chronicity of skin lesions.AimTo evaluate the relationship between plasma periostin levels and severity and chronicity of AD in children.Material and methodsThe study population consisted of 29 children with atopic dermatitis without concomitant allergic disease such as asthma or allergic rhinitis and 31 healthy controls. Data of demographic features, serum eosinophil, total IgE and skin prick test results were collected through the patient’s medical records. The severity of the disease was assessed by the SCORAD index. Serum periostin levels were measured with a human periostin ELISA kit.ResultsThe mean ages of the AD patients and the control group participants were 80.7 ±52.8 and 90.3 ±41.6 months, respectively. Mean plasma periostin levels were 63.0 ±19.0 ng/ml in AD patients, and 23.6 ±7.3 in healthy controls, and there was a statistically significant difference between the two groups (p = 0.001). Plasma periostin level did not vary according to total IgE or serum eosinophil count (p > 0.05). Age of onset and duration of symptoms also were not correlated with plasma periostin levels. Although there was a positive relationship between plasma periostin level and the SCORAD index of patients, it was not statistically significant (r = 0.19, p > 0.05).ConclusionsThis study showed that plasma periostin levels were increased in children with atopic dermatitis. Periostin may have a partial role in the pathogenesis of atopic dermatitis, but it is not associated with severity or chronicity in children with atopic dermatitis.

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Section: Discussioncontrasting

confidence: 99%

“…Various biomarkers such as TARC, TSLP, IL-31, and IL-33 have been investigated to evaluate the pathogenesis of AD and to predict severity and chronicity. TARC and TLSP were found to be related to AD severity and chronicity [21]. Our study has some limitations.…”

Section: Discussionmentioning

confidence: 93%

Evaluation of periostin level for predicting severity and chronicity of childhood atopic dermatitis

Özçeker

Yücel

Sipahi

et al. 2019

pdia

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“…However, few studies have attempted to investigate how osteoblasts initiate osteoclasts aggregation and differentiation, promote the formation of the eruption channel, or explored whether autophagy occurs during this process. Periostin (POSTN) is a multifunctional soluble intercellular matrix protein that is mainly expressed in the periosteum and regulates osteoblasts to increase value, differentiation, and adherence (Uysal et al, 2017;Zeng et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Effect ofPeriostinSilencing on the Autophagy of Osteoblasts

Han

Cai²

2019

Cellular Reprogramming

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The objective of the present work was to investigate the effect of Periostin (POSTN) silencing on autophagy in osteoblasts, and provide an experimental basis for studying the mechanism of dental eruption. The cells were divided into the following four groups according to their viral number: the NC group, pFU-GW-016PSC53349-1; group KD1, LVpFU-GW-016PSC66471-1; group KD2, LVpFU-GW-016PSC66472-1; and group KD3, LVpFU-GW-016PSC66473-1. The lentiviral vector was infected at MOI = 100 in the ENi.S medium containing 5 g/mL Polybrene. The target gene expression was observed by a Celigo Ò Image Cytometer at 72 hours after infection, and the positive rate of fluorescence was noted. A two-step method of quantitative real-time PCR (qRT-PCR) was used to detect the silencing effect of POSTN. Western blotting was then performed to assess the expression of autophagy-related proteins Beclin-1 and LC3 in the group showing the best gene silencing effects. The experimental results showed that there was strong green fluorescence in group KD3. As confirmed via qRT-PCR analysis, the POSTN silencing efficiency in group KD3 reached 92.1%. The Western blotting revealed that the expression of Beclin-1 protein in group KD3 was significantly higher than that in the NC group. However, the LC3 protein expression was not significantly different from that of the control group. The lentiviral vector targeting POSTN in osteoblasts was constructed successfully. In addition, the expression of autophagy protein in mouse osteoblasts increased after POSTN silencing. This finding may provide new approaches for understanding the molecular signal transduction of POSTN during the tooth eruption process.

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“…As an epithelium-derived cytokine, TSLP activates dendritic cells to induce a Th2 response, resulting in the production of Th2 cytokines, such as IL-4 and IL-13 [26]. TARC and TSLP are highly expressed in serum and AD lesions, leading to Th2-mediated allergic inflammation in the development of AD [27,28]. This study showed that the secretion levels of RANTES, TARC, and TSLP were dose-dependently reduced by NHGR treatment in TNF-α and IFN-γ-induced HaCaT cells.…”

Section: Discussionmentioning

confidence: 99%

Siraitia grosvenorii Residual Extract Attenuates Atopic Dermatitis by Regulating Immune Dysfunction and Skin Barrier Abnormality

et al. 2020

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Atopic dermatitis is a persistent inflammatory skin disorder. Siraitia grosvenorii fruits (monk fruit or nahangwa in Korean, NHG) are used as a natural sweetener and as a traditional medicine for the treatment of asthma and bronchitis. We evaluated the activity of S. grosvenorii residual extract (NHGR) on allergic inflammation of atopic dermatitis in a Dermatophagoides farinae mite antigen extract (DfE)-treated NC/Nga murine model and in vitro. Oral administration of NHGR significantly reduced epidermal hyperplasia and inflammatory cell infiltration in the skin lesions of DfE-induced atopic dermatitis, as well as the dermatitis severity score. NHGR reduced serum immunoglobulin E levels. Splenic concentrations of IFN-γ, interleukin (IL)-4, IL-5, and IL-13 were reduced by NHGR administration. Immunohistofluorescence staining showed that NHGR administration increased the protein levels of claudin-1, SIRT1, and filaggrin in atopic dermatitis skin lesions. In addition, NHGR inhibited the phosphorylation of mitogen-activated protein kinases and decreased filaggrin and chemokine protein expression in TNF-α/IFN-γ-induced human keratinocytes. Moreover, NHGR also inhibited histamine in mast cells. The quantitative analysis of NHGR revealed the presence of grosvenorine, kaempferitrin, and mogrosides. These results demonstrate that NHGR may be an efficient therapeutic agent for the treatment of atopic dermatitis.

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The Relationship Between Serum TARC, TSLP and POSTN Levels and Childhood Atopic Dermatitis

Abstract: Serum POSTN, TARC, and TSLP were higher in children with atopic dermatitis. Serum TARC and TSLP levels might be used as biomarkers to predict severe atopic dermatitis and serum POSTN to predict atopy and disease chronicity.

Cited by 15 publications

References 0 publications

Evaluation of periostin level for predicting severity and chronicity of childhood atopic dermatitis

Evaluation of periostin level for predicting severity and chronicity of childhood atopic dermatitis

Effect ofPeriostinSilencing on the Autophagy of Osteoblasts

Siraitia grosvenorii Residual Extract Attenuates Atopic Dermatitis by Regulating Immune Dysfunction and Skin Barrier Abnormality

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