The relationship between trace mineral concentrations of amniotic fluid with placenta traits in the pregnancy toxemia Ghezel ewes

Olfati, Ali; Moghaddam, Gholamali; Kor, Nasroallah Moradi; Baradaran, Behzad

doi:10.1016/j.apjr.2016.06.003

Cited by 3 publications

(2 citation statements)

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“…To mention however, sheep, for the most part, have researchers surgically perform on their placenta when the fetus has reached a substantial weight and the maturity is quite high. In contrast though, the mouse model usually has the scientists genetically modify the mouse placenta and operate the surgical procedures earlier in the fetal development [41]. Nevertheless, the previous testing for nutrient metabolism and placental nutrient transfer on the sheep placenta was only made possible due to the shared physiological traits between the sheep and human placenta.…”

Section: Mouse and Sheep Modelsmentioning

confidence: 99%

Drug transport across the human placenta: review of placenta-on-a-chip and previous approaches

2019

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One contribution of 15 to a theme issue 'Bioengineering in women's health, volume 2: pregnancy-from implantation to parturition'.In the past few decades, the placenta became a very controversial topic that has had many researchers and pharmacists discussing the significance of the effects of pharmaceutical drug intake and how it is a possible leading cause towards birth defects. The creation of an in vitro microengineered model of the placenta can be used to replicate the interactions between the mother and fetus, specifically pharmaceutical drug intake reactions. As the field of nanotechnology significantly continues growing, nanotechnology will become more apparent in the study of medicine and other scientific disciplines, specifically microengineering applications. This review is based on past and current research that compares the feasibility and testing of the placenta-on-a-chip microengineered model to the previous and underdeveloped in vivo and ex vivo approaches. The testing of the practicality and effectiveness of the in vitro, in vivo and ex vivo models requires the experimentation of prominent pharmaceutical drugs that most mothers consume during pregnancy. In this case, these drugs need to be studied and tested more often. However, there are challenges associated with the in vitro, in vivo and ex vivo processes when developing a practical placental model, which are discussed in further detail.

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Section: Mouse and Sheep Modelsmentioning

confidence: 99%

Drug transport across the human placenta: review of placenta-on-a-chip and previous approaches

2019

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“…Clearly, studying placental pathology in vivo, including PM, is possible, but difficult due to multiple reasons. Therefore, different models in vivo 13 , 14 , ex vivo 15 , 16 , and in vitro 17 , 18 have been developed, including various models to study transport across the placental barrier. Due to some differences in placenta anatomy in animals and humans, there are some challenges for in vivo studies using animal models.…”

Section: Introductionmentioning

confidence: 99%

3D microfluidics-assisted modeling of glucose transport in placental malaria

Mosavati

Oleinikov

2022

Sci Rep

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The human placenta is a critical organ, mediating the exchange of nutrients, oxygen, and waste products between fetus and mother. Placental malaria (PM) resulted from Plasmodium falciparum infections causes up to 200 thousand newborn deaths annually, mainly due to low birth weight, as well as 10 thousand mother deaths. In this work, a placenta-on-a-chip model is developed to mimic the nutrient exchange between the fetus and mother under the influence of PM. In this model, trophoblasts cells (facing infected or uninfected blood simulating maternal blood and termed “trophoblast side”) and human umbilical vein endothelial cells (facing uninfected blood simulating fetal blood and termed “endothelial” side) are cultured on the opposite sides of an extracellular matrix gel in a compartmental microfluidic system, forming a physiological barrier between the co-flow tubular structure to mimic a simplified maternal–fetal interface in placental villi. The influences of infected erythrocytes (IEs) sequestration through cytoadhesion to chondroitin sulfate A (CSA) expressed on the surface of trophoblast cells, a critical feature of PM, on glucose transfer efficiency across the placental barrier was studied. To create glucose gradients across the barrier, uninfected erythrocyte or IE suspension with a higher glucose concentration was introduced into the “trophoblast side” and a culture medium with lower glucose concentration was introduced into the “endothelial side”. The glucose levels in the endothelial channel in response to CSA-adherent erythrocytes infected with CS2 line of parasites in trophoblast channel under flow conditions was monitored. Uninfected erythrocytes served as a negative control. The results demonstrated that CSA-binding IEs added resistance to the simulated placental barrier for glucose perfusion and decreased the glucose transfer across this barrier. The results of this study can be used for better understanding of PM pathology and development of models useful in studying potential treatment of PM.

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