The Relationships among Reproductive Endpoints in Swiss Mice, Using the Reproductive Assessment by Continuous Breeding Database

Chapin, Robert E.; Sloane, Richard A.; Haseman, Joseph K.

doi:10.1006/faat.1997.2341

Cited by 86 publications

(34 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sperm motility assessed by CASA is a common measure of sperm quality in fertility clinics (Oehninger et al, 2000) and low percent motile sperm (%MOT) has been associated with reduced fertility (Chapin et al, 1997). Reduction in sperm motility has been associated with smoking >20 cigarettes/day (Ramlau-Hansen et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Differential sensitivity of male germ cells to mainstream and sidestream tobacco smoke in the mouse

Polyzos

Schmid

Piña-Guzmán

et al. 2009

Toxicology and Applied Pharmacology

View full text Add to dashboard Cite

Cigarette smoking in men has been associated with increased chromosomal abnormalities in sperm and with increased risks for spontaneous abortions, birth defects and neonatal death.Little is known, however, about the reproductive consequences of paternal exposure to secondhand smoke. We used a mouse model to investigate the effects of paternal exposure to sidestream (SS) smoke, the main constituent of second-hand smoke, on the genetic integrity and function of sperm, and to determine whether male germ cells were equally sensitive to mainstream (MS) and SS smoke. A series of sperm DNA quality and reproductive endpoints were investigated after exposing male mice for two weeks to MS or SS smoke. Our results indicated that: (i) only SS smoke significantly affected sperm motility; (ii) only MS smoke induced DNA strand breaks in sperm; (iii) both MS and SS smoke increased sperm chromatin structure abnormalities; and (iv) MS smoke affected both fertilization and the rate of early embryonic development, while SS smoke affected fertilization only. These results show that MS and SS smoke have differential effects on the genetic integrity and function of sperm and provide further evidence that male exposure to second-hand smoke, as well as direct cigarette smoke, may diminish a couple's chance for a successful pregnancy and the birth of a healthy baby.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Exposures of mice to a variety of environmental agents, including components of tobacco smoke, have been shown to affect sperm motility (Chapin et al, 1997;Elangovan et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Differential sensitivity of male germ cells to mainstream and sidestream tobacco smoke in the mouse

Polyzos

Schmid

Piña-Guzmán

et al. 2009

Toxicology and Applied Pharmacology

View full text Add to dashboard Cite

show abstract

“…To use toxicology data derived from animal studies to advantage in risk assessment, it is critical to identify and understand species-specific differences in physiology and metabolism that may affect the response to the toxicant in question. Rodent models have, for example, been used to determine the relationship between sperm end points and function (fertility) (Chapin et al, 1997). Determining that a substance is toxic to the male reproductive system is only the first step: The next step is to examine its mechanisms of toxicity.…”

Section: The Contribution Of Experimental Modelsmentioning

confidence: 99%

Environmental Toxicants Induced Male Reproductive Disorders: Identification and Mechanism of Action

Jana¹,

Sen²

2012

Toxicity and Drug Testing

View full text Add to dashboard Cite

“…Aliquots of the epididymal suspension were fixed in 5% paraformaldehyde and counted using a hemocytometer. Sperm motility was assessed by counting the number of sperm that showed tail motion 29 and expressing this as a percentage of the total number of sperm (minimum of 100) counted. To assess sperm morphology a minimum of 200 sperm were counted with the numbers of morphologically normal sperm and those with abnormal heads or flagellae being recorded.…”

Section: Sperm Analysismentioning

confidence: 99%

Male Infertility and DNA Damage in Doppel Knockout and Prion Protein/Doppel Double-Knockout Mice

Paisley

Banks

Selfridge

et al. 2004

The American Journal of Pathology

View full text Add to dashboard Cite

The prion protein (PrP) and Doppel (Dpl) have many structural and biochemical properties in common, leading to the suggestion that the lack of an obvious phenotype in PrP-deficient mice maybe because of compensation by Dpl. To test this hypothesis and also investigate the function of Dpl we have generated Prnd ؊/؊ and Prnp ؊/؊ /Prnd ؊/؊ mouse lines. Both develop normally and display an identical male sterility phenotype that differs from that reported for another Prnd ؊/؊ mouse line. Sperm from both our mutant lines were present at normal concentrations, had normal motility, and no morphological abnormalities. Despite only rarely fertilizing oocytes in vivo, because of an inability to perform the acrosome reaction, mutant sperm were capable of fertilization in vitro, albeit at reduced rates compared to wild type. Elevated levels of oxidative DNA damage were found in both types of mutant sperm and resulting embryos failed at an early stage. Therefore we found no evidence that Dpl compensates for the loss of PrP function in mutant mouse lines, but it does have an important anti-oxidant function necessary for sperm integrity and male fertility.

show abstract

The Relationships among Reproductive Endpoints in Swiss Mice, Using the Reproductive Assessment by Continuous Breeding Database

Cited by 86 publications

References 12 publications

Differential sensitivity of male germ cells to mainstream and sidestream tobacco smoke in the mouse

Differential sensitivity of male germ cells to mainstream and sidestream tobacco smoke in the mouse

Environmental Toxicants Induced Male Reproductive Disorders: Identification and Mechanism of Action

Male Infertility and DNA Damage in Doppel Knockout and Prion Protein/Doppel Double-Knockout Mice

Contact Info

Product

Resources

About