The release kinetics, antimicrobial activity and cytocompatibility of differently prepared collagen/hydroxyapatite/vancomycin layers: Microstructure vs. nanostructure

Suchý, Tomáš; Šupová, Monika; Klapková, Eva; Adámková, Václava; Závora, Jan; Žaloudková, Margit; Rýglová, Šárka; Ballay, Rastislav; Denk, František; Pokorný, Marek; Sauerová, Pavla; Kalbáčová, Marie Hubálek; Horny, Lukas; Vesely, Jan; Voňavková, Tereza; Průša, Richard

doi:10.1016/j.ejps.2017.01.032

Cited by 32 publications

(31 citation statements)

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“…To date, the application of vancomycin-releasing collagen sponges as wound dressings has not been studied to any significant extent. Current approaches involve either the incorporation of other antibiotics into collagen sponges [1][2][3] or the use of vancomycin as one element of hydroxyapatite beads in the treatment of musculoskeletal injuries [4,5]. Moreover, various studies have described the administration of a vancomycin powder directly on surgical wounds so as to prevent wound infection following spinal, cardiac, or vascular surgery, with good effects [6,7].…”

Section: Introductionmentioning

confidence: 99%

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Vancomycin-releasing cross-linked collagen sponges as wound dressings

Hartinger

Mitáš

Mlček

et al. 2019

Bosn J of Basic Med Sci

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The study presents a novel vancomycin-releasing collagen wound dressing derived from Cyprinus carpio collagen type I cross-linked with carbodiimide which retarded the degradation rate and increased the stability of the sponge. Following lyophilization, the dressings were subjected to gamma sterilization. The structure was evaluated via scanning electron microscopy images, micro-computed tomography, and infrared spectrometry. The structural stability and vancomycin release properties were evaluated in a phosphate buffer solution. Microbiological testing and a rat model of a wound infected with methicillin-resistant Staphylococcus aureus (MRSA) were then employed to test the efficacy of the treatment of the infected wound. Following an initial mass loss due to the release of vancomycin, the sponges remained stable. After 7 days of exposure in phosphate buffered saline (37°C), 60% of the material remained with a preserved collagen secondary structure together with a high degree of open porosity (over 80%). The analysis of the release of the vancomycin revealed the homogeneous distribution of the antibiotic both across and between the sponges. The release of vancomycin was retarded as proved by in vitro testing and further confirmed by the animal model from which measurable concentrations were observed in blood samples 24 hours after the subcutaneous implantation of the sponge, which was more than observed following i. p. administration. The sponge was also highly effective in terms of reducing the number of colony-forming units in biopsies extracted from the infected wounds 4 days following the inoculation of the wounds with the MRSA solution.

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Section: Introductionmentioning

confidence: 99%

“…Mammal collagens, such as calf or bovine, are the most used collagen types for biomaterial engineering [4,5].…”

Section: Introductionmentioning

confidence: 99%

Vancomycin-releasing cross-linked collagen sponges as wound dressings

Hartinger

Mitáš

Mlček

et al. 2019

Bosn J of Basic Med Sci

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“…In this step, PEO was fully leached out, so the final concentration of COL/HAp in the layer was 85/15 (w/w). The preparation of spinning solution is described in detail elsewhere [18].…”

Section: Methodsmentioning

confidence: 99%

“…This paper deals with the preparation of composite nanofibrous layers composed of COL/HAp/PEO (polyethylene oxide) using the electrostatic spinning method. The composition and structure of the composite layer and its suitability for its intended application, i.e., implant surface modification, as well as the suitability of its kinetic drug release properties, were tested and verified in our previous research [18,19]. Moreover, the cytocompatibility of electrospun nanofibrous COL/HAp layers was evaluated in our previous studies using human osteoblastic cells in direct contact with the layers or in 24-h infusions thereof [18,19].…”

Section: Introductionmentioning

confidence: 90%

“…The composition and structure of the composite layer and its suitability for its intended application, i.e., implant surface modification, as well as the suitability of its kinetic drug release properties, were tested and verified in our previous research [18,19]. Moreover, the cytocompatibility of electrospun nanofibrous COL/HAp layers was evaluated in our previous studies using human osteoblastic cells in direct contact with the layers or in 24-h infusions thereof [18,19]. No cytotoxic effects were indicated in the case of layers without antibiotics as well as in the case of layers impregnated with vancomycin or combination of vancomycin and gentamicin.…”

Section: Introductionmentioning

confidence: 95%

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Surface Treatment of Acetabular Cups with a Direct Deposition of a Composite Nanostructured Layer Using a High Electrostatic Field

et al. 2020

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A composite nanofibrous layer containing collagen and hydroxyapatite was deposited on selected surface areas of titanium acetabular cups. The layer was deposited on the irregular surface of these 3D objects using a specially developed electrospinning system designed to ensure the stability of the spinning process and to produce a layer approximately 100 micrometers thick with an adequate thickness uniformity. It was verified that the layer had the intended nanostructured morphology throughout its entire thickness and that the prepared layer sufficiently adhered to the smooth surface of the model titanium implants even after all the post-deposition sterilization and stabilization treatments were performed. The resulting layers had an average thickness of (110 ± 30) micrometers and an average fiber diameter of (170 ± 49) nanometers. They were produced using a relatively simple and cost-effective technology and yet they were verifiably biocompatible and structurally stable. Collagen- and hydroxyapatite-based composite nanostructured surface modifications represent promising surface treatment options for metal implants.

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