The reliable and rapid polymerase chain reaction (PCR) diagnosis for Xanthomonas axonopodis pv. punicae in pomegranate

Mondal, Kalyan K.; Rajendran, T. P.; Phaneendra, C.; Mani, Chandra; Sharma, Jyotsana; Shukla, Richa; Pooja, .; Verma, G. S.; Kumar, Rishikesh; Singh, Dalbir; Kumar, Ashok; Saxena, Anupam; Jain, Richa

doi:10.5897/ajmr12.543

Cited by 11 publications

(4 citation statements)

References 9 publications

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“…However, the sampling of plant tissues to be tested for PCR detection need to be carried out very carefully as the blight bacterium, Xap is a mesophyll localized not systemic one. Application of this protocol may pave the way for a rapid and efficient management of the disease in pomegranate fields (19). The results of restriction enzymes digestion using Hinf I, Hae III and Rsa I of the 1.5 kb fragment obtained across all strains, and the amplified product of 16s rRNA, are shown in Figure 4.…”

Section: Axopodia Pv Punicae By Using the 16s Rrna Primermentioning

confidence: 99%

PCR-RFLP Analysis of the 16s Rrna and Its Regions in Bacterial Blight (Xanthomonas Axonopodis Pv. Punicae) Across Pomegranate Farms in Kurdistan Regions-Iraq.

Sinjare¹,

Jubrael²

2023

Iraqi J. Agric. Sci.

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Bacterial blight caused by Xanthomonas axonopodis pv. punicae (Xap) is a major biotic diseases in pomegranate (Punica granatum L.). In the field survey which lasted 2 year in six different geographical locations, Xap strains from the severely infected plant material collected including DuhokCcenter, around Erbil, Zaxo, south of Duhok, Akre, and Amedi. Xanthomonas was detected from infected plant material and its identity was confirmed by morphological, Microbial and Molecular Characterization. To study its genetic variability and phylogenetic relationship two important loci were targeted namely ITS region of 16s rDNA, 16S rRNA and then a PCR-RFLP was performed for PCR product of 16s rRNA loci using Hinf I, Hae III, and Rsa I restriction enzymes. The PCR-RFLP showed the genetic similarity coefficient ranging from 1.00 to 3.73, and the dendrogram grouped all tested strains in 4 clusters. The result revealed that this disease is in blooming stage in the country which was thought that has not been recorded before in pomegranate. To the best of our knowledge this is the first record of Xap. In Kurdistan/ Iraq therefore, further studies are needed to be performed to manage this hazardous bacterium.

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Section: Axopodia Pv Punicae By Using the 16s Rrna Primermentioning

confidence: 99%

PCR-RFLP Analysis of the 16s Rrna and Its Regions in Bacterial Blight (Xanthomonas Axonopodis Pv. Punicae) Across Pomegranate Farms in Kurdistan Regions-Iraq.

Sinjare¹,

Jubrael²

2023

Iraqi J. Agric. Sci.

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“…Sharma et al [15] revealed that pathogenicity through spray inoculation showed symptoms 14 days post injection. The pathogenicity test of the bacterial blight pathogen XAP was described by several researchers, viz., [16]. The excessive or deficiency of minerals and nutrients causes various problems in plants which are determined by the amount of elements, kind of disease, element form, and environmental situations that interrupt the appearance of disease.…”

Section: Determination Of Phosphorus Potassium Zinc Iron Manganese An...mentioning

confidence: 99%

Biochemical Characterization and Effect of Bacterial Blight on Micro and Macro Nutrient Concentration in Pomegranate

RASHID,

CHOHAN,

SAJID

et al. 2022

PCBMB

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Bacterial blight is a serious disease of pomegranate. The disease affects the yield and quality of pomegranate fruits. In this present work, we investigated the biochemical characterization and role of micro and macro nutrients in diseased and healthy leaves of pomegranate. Pathogen was isolated infected leaves, twigs and fruits and grown on different culture media. Different biochemical tests (Gram staining, KOH, catalase, oxidase, starch hydrolysis, gelatin liquefaction) were performed to identify the bacteria. All these tests confirm the presence of Xanthomonas axonopodis pv punicae. Pathogenicity was done in the greenhouse using three different methods (Pin-pricked, infiltration and spray method) and symptoms appeared in 8 days; and infiltration method showed early symptoms compared to the spray and pin prick method. Ionic contents (Nitrogen, Potassium, Phosphorus, Magnesium, Calcium, Iron, Boron, Zinc and Copper) are reduced by using these nutrients for the growth, multiplication and survival of pathogenic bacteria. Proper application of these nutrients helps the host plant to improve its physiological and biochemical development and resistance to bacterial blight disease in pomegranate.

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“…Estos patógenos a menudo causan daño en tejidos, lo que hace que la fruta pierda su valor comercial. Un porcentaje importante de pérdidas de fruta en cosecha y postcosecha es atribuido a enfermedades causadas por diversas especies de hongos y bacterias (Mondal et al, 2012). Adicionalmente a las enfermedades de tipo biótico se suman desórdenes fisiológicos como agrietamiento de epidermis, lesiones por frío, escaldadura de la piel del fruto y perdida excesiva de peso (Caleb, Opara y Witthuhn, 2012).…”

Section: Plagas Y Enfermedades De Importancia En La Regiónunclassified

GRANADO (Punica granatum L.), UN CULTIVO CON POTENCIAL PRODUCTIVO: REVISIÓN Y SITUACIÓN EN COLOMBIA

Aguilera-Arango

Ortiz

Burbano-Erazo

et al. 2020

Trop Subtrop Agroecosyst

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Background. The worldwide market and consumption of pomegranate fruit (Punica granatum L.) has grown considerably, due to its nutraceutical properties, with a high antioxidant content, generating favorable effects for human health. In Colombia some semi-commercial crops are reported in the departments of Boyacá, Huila and Valle del Cauca. However, it is unknown about its productive behavior, management practices, physiology, development of the phenological stages and pomological characteristics of fruits of native genotypes. Objective. Conduct a review of the aspects related to the crop of pomegranate, analyze possible limitations for its production in Colombia and present information on the pomological characteristics of pomegranate plants grown in home gardens in two regions of Colombia. Methodology. Information was collected from documentary sources related to recommendations applicable to pomegranate cultivation in Colombia. A pomological characterization of fruits of native genotypes from two different agro-ecological zones of the national territory was made. Results. Native genotypes have similar pomological characteristics, but differ considerably from commercial genotypes, the latter being superior in accordance with market requirements for export. Implications. The low technical knowledge on the part of the farmers allows that there are deficiencies in the management of limitations of this crop. Conclusions. The recommendations set forth, complemented with pomological characterization, generate knowledge that can be implemented by producers in Colombia. More research is required if the crop is to be projected as a promising economic alternative to the region.

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The reliable and rapid polymerase chain reaction (PCR) diagnosis for Xanthomonas axonopodis pv. punicae in pomegranate

Cited by 11 publications

References 9 publications

PCR-RFLP Analysis of the 16s Rrna and Its Regions in Bacterial Blight (Xanthomonas Axonopodis Pv. Punicae) Across Pomegranate Farms in Kurdistan Regions-Iraq.

PCR-RFLP Analysis of the 16s Rrna and Its Regions in Bacterial Blight (Xanthomonas Axonopodis Pv. Punicae) Across Pomegranate Farms in Kurdistan Regions-Iraq.

Biochemical Characterization and Effect of Bacterial Blight on Micro and Macro Nutrient Concentration in Pomegranate

GRANADO (Punica granatum L.), UN CULTIVO CON POTENCIAL PRODUCTIVO: REVISIÓN Y SITUACIÓN EN COLOMBIA

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