The Response of Human Peripheral Lymphocytes to Various Mitogenic Agents in vitro

Naspitz, Charles K.; Richter, M.

doi:10.1159/000230055

Cited by 12 publications

(4 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our studies we have noted no time or dose related supression of growth or label incorporation [unpublished data]. It is of note that the use of long and large pulses continue to be reported [11,12,14,30,32]. Our data reflect the expected early peak in RNA synthesis in PHA stimulated cultures, followed by a peak in DNA synthesis.…”

Section: Discussionsupporting

confidence: 67%

“…Tritiated thymidine pulse labeling has been used to measure DNA syn Stimulated Guinea-Pig Lymph Node Lymphocyte Cultures 405 thesis in many laboratories [11,12,14,[30][31][32]. Inaccuracies in this technique have been attributed to the lack of assessment of intra cellular precursor pools [28], the cytotoxicity of tritium exposure [31], and the gradual conversion of thymidine to thymine [33,34].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Cell Dynamics in Phytohemagglutinin (PHA) Stimulated Guinea-Pig Lymph Node Lymphocyte Cultures

Eurenius¹,

McIntyre²

1970

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Cell Dynamics in Phytohemagglutinin (PHA) Stimulated Guinea-Pig Lymph Node Lymphocyte Cultures

Eurenius¹,

McIntyre²

1970

Int Arch Allergy Immunol

View full text Add to dashboard Cite

“…1966; Schwartz, 1967Schwartz, , 1968N a spitz, 1968). Our findings for mouse lymphocytes are in good general agreement with those reported for lymphocyte cultures derived from human sources (W ii.son, 1966;Naspitz, 1968;and others) and for lymphoid cells from the rat (Schwartz, 1968). As is the case with PHA, our data clearly indicate that an optimal concentration range exists and that above 0.002 ml/ml PWM depresses mitotic activity of cultures derived from lymph-node cells.…”

Section: Discussionsupporting

confidence: 92%

The in vitro stimulation of lymphocytes from peripheral blood and lymph nodes of the laboratory mouse

Bryan¹,

Hybertson²

1972

Cytogenet Genome Res

View full text Add to dashboard Cite

The results of comparative studies of the efficacy of phytohemagglutinin (PHA-P) and pokeweed mitogen (PWM) as mitotic stimulators for in vitro cultures of mouse lymphocytes are reported. These studies have established that clearly defined optimal concentration ranges exist for each mitogen and that higher than optimal levels of either mitogen exert an inhibitory effect on cell proliferative activities. The levels of mitotic stimulation achieved with peripheral-blood cultures and with cultures of lymph-node lymphocytes should facilitate a variety of cytogenetic studies not heretofore practicable with the mouse.

show abstract

“…Four hours before harvest, the cultures received 20 ,«Ci 3H-thymidine (specific activity 2.0 Ci/mmole; New England Nuclear Corp., Boston, Mass., USA), and 3H-thymidine incorporation into DNA was determined [25].…”

Section: Methodsmentioning

confidence: 99%

Ultrastructural Features of Phytohemagglutinin and Concanavalin A – Responsive Lymphocytes in Chronic Lymphocytic Leukemia

Douglas¹,

Cohnen²,

König³

et al. 1973

Acta Haematol

View full text Add to dashboard Cite

Peripheral blood lymphocytes from patients with chronic lymphocytic leukemia (CLL) had diminished and/or delayed in vitro responses to both phytohemagglutinin (PHA) and concanavalin A (Con A) as determined by thymidine incorporation into DNA, over a 9-day incubation period. The cytoarchitectural features of CLL lymphocytes stimulated by each of the mitogens were similar to those observed in transformed normal cells. However, as shown by planimetric measurements, the mean cell area and the nuclear, cytoplasmic, and mitochondrial areas were diminished in comparison to PHA-stimulated normal cells. The data suggest that some CLL lymphocytes which transform with PHA or Con A are residual normal thymus-dependent (T) lymphocytes, and others may be derived from the metabolically defective leukemic cell population

show abstract

The Response of Human Peripheral Lymphocytes to Various Mitogenic Agents in vitro

Cited by 12 publications

References 4 publications

Cell Dynamics in Phytohemagglutinin (PHA) Stimulated Guinea-Pig Lymph Node Lymphocyte Cultures

Cell Dynamics in Phytohemagglutinin (PHA) Stimulated Guinea-Pig Lymph Node Lymphocyte Cultures

The in vitro stimulation of lymphocytes from peripheral blood and lymph nodes of the laboratory mouse

Ultrastructural Features of Phytohemagglutinin and Concanavalin A – Responsive Lymphocytes in Chronic Lymphocytic Leukemia

Contact Info

Product

Resources

About