The response regulator 2 mediates ethylene signalling and hormone signal integration in Arabidopsis

Hass, C.; Lohrmann, Jens; Albrecht, Verónica; Sweere, Uta; Hummel, Florian; Yoo, Sang Dong; Hwang, Ildoo; Zhu, Tong; Schäfer, Eberhard; Kudla, Jörg; Harter, Klaus

doi:10.1038/sj.emboj.7600337

Cited by 199 publications

(260 citation statements)

References 36 publications

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“…Overexpression of either ARR1 or ARR2 in Arabidopsis plants results in increased sensitivity to cytokinin (Hwang and Sheen, 2001;Sakai et al, 2001). ARR2, however, has also been proposed to play a role in mediating ethylene signal transduction based on the analysis of a transposon-induced mutation in the Landsberg erecta background (Hass et al, 2004). The relatively weak phenotypes revealed by the analysis of individual ARR mutations may be the result of functional overlap among the type-B ARRs, a hypothesis consistent with the gene expression patterns Tajima et al, 2004).…”

Section: Introductionmentioning

confidence: 67%

Multiple Type-B Response Regulators Mediate Cytokinin Signal Transduction in Arabidopsis

et al. 2005

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Type-B Arabidopsis thaliana response regulators (ARRs) are transcription factors that function in the final step of twocomponent signaling systems. To characterize their role in plant growth and development, we isolated T-DNA insertions within six of the genes (ARR1, ARR2, ARR10, ARR11, ARR12, and ARR18) from the largest subfamily of type-B ARRs and also constructed various double and triple combinations of these mutations. Higher order mutants revealed progressively decreased sensitivity to cytokinin, including effects on root elongation, lateral root formation, callus induction and greening, and induction of cytokinin primary response genes. The triple mutant arr1,10,12 showed almost complete insensitivity to cytokinin under many of the assay conditions used. By contrast, no significant change in the sensitivity to ethylene was found among the mutants examined. These results indicate that there is functional overlap among the type-B ARRs and that they act as positive regulators of cytokinin signal transduction.

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Section: Introductionmentioning

confidence: 67%

Multiple Type-B Response Regulators Mediate Cytokinin Signal Transduction in Arabidopsis

et al. 2005

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“…For instance, protein ARR2 in Arabidopsis thaliana is associated with "response to ethylene" (GO:0009723) both positively on the basis of a paper by Hass et al [ 24 ] and negatively based on a paper by Mason et al [ 25 ]. The latter discusses this contradiction as follows:…”

Section: Gene Ontology Annotationsmentioning

confidence: 99%

“…Hass et al [ 24 ] reported a reduction in the ethylene sensitivity of seedlings containing an arr2 loss-of-function mutation. By contrast, we observed no signifi cant difference from the wild type in the seedling ethylene response when we tested three independent arr2 insertion mutants, including the same mutant examined by Hass et al [ 24 ].…”

Section: Modifi Cation Of Annotation Meaning By Qualifi Ers 32 Negatmentioning

confidence: 99%

Gene Ontology: Pitfalls, Biases, and Remedies

Gaudet

Dessimoz

2016

Methods in Molecular Biology

144

103

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The Gene Ontology (GO) is a formidable resource, but there are several considerations about it that are essential to understand the data and interpret it correctly. The GO is suffi ciently simple that it can be used without deep understanding of its structure or how it is developed, which is both a strength and a weakness. In this chapter, we discuss some common misinterpretations of the ontology and the annotations. A better understanding of the pitfalls and the biases in the GO should help users make the most of this very rich resource. We also review some of the misconceptions and misleading assumptions commonly made about GO, including the effect of data incompleteness, the importance of annotation qualifi ers, and the transitivity or lack thereof associated with different ontology relations. We also discuss several biases that can confound aggregate analyses such as gene enrichment analyses. For each of these pitfalls and biases, we suggest remedies and best practices.

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“…For example, the inhibitory effect of cytokinin on etiolated hypocotyl elongation, as well as root elongation, has been linked to cytokinininduced ethylene production (Cary et al, 1995). In addition, the Type-B RR ARR2 has been identified as a transcription factor (Sakai et al, 2000;Lohrmann et al, 2001) that acts as a positive regulator of both cytokinin signaling (Hwang and Sheen, 2001) and ethylene signaling (Hass et al, 2004). …”

Section: Other Phytohormone-related Changesmentioning

confidence: 99%

A Transcriptome-Based Characterization of Habituation in Plant Tissue Culture

Pischke¹,

Huttlin²,

Hegeman³

et al. 2006

Plant Physiology

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For the last 50 years, scientists have recognized that varying ratios of the plant hormones cytokinin and auxin induce plant cells to form particular tissues: undifferentiated calli, shoot structures, root structures, or a whole plant. Proliferation of undifferentiated callus tissue, greening, and the formation of shoot structures are all cytokinin-dependent processes. Habituation refers to a naturally occurring phenomenon whereby callus cultures, upon continued passage, lose their requirement for cytokinin. Earlier studies of calli with a higher-than-normal cytokinin content indicate that overproduction of cytokinin by the culture tissues is a possible explanation for this acquired cytokinin independence. A transcriptome-based analysis of a well established habituated Arabidopsis (Arabidopsis thaliana) cell culture line was undertaken, to explore genome-wide expression changes underlying the phenomenon of habituation. Increased levels of expression of the cytokinin receptor CRE1, as well as altered levels of expression of several other genes involved in cytokinin signaling, indicated that naturally acquired deregulation of cytokinin-signaling components could play a previously unrecognized role in habituation. Up-regulation of several cytokinin oxidases, downregulation of several known cytokinin-inducible genes, and a lack of regulation of the cytokinin synthases indicated that increases in hormone concentration may not be required for habituation. In addition, up-regulation of the homeodomain transcription factor FWA, transposon-related elements, and several DNA-and chromatin-modifying enzymes indicated that epigenetic changes contribute to the acquisition of cytokinin habituation.

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The response regulator 2 mediates ethylene signalling and hormone signal integration in Arabidopsis

Cited by 199 publications

References 36 publications

Multiple Type-B Response Regulators Mediate Cytokinin Signal Transduction in Arabidopsis

Multiple Type-B Response Regulators Mediate Cytokinin Signal Transduction in Arabidopsis

Gene Ontology: Pitfalls, Biases, and Remedies

A Transcriptome-Based Characterization of Habituation in Plant Tissue Culture

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