The rice ethylene response factor OsERF83 positively regulates disease resistance to Magnaporthe oryzae

Tezuka, Daisuke; Kawamata, Aya; Kato, Hideki; Saburi, Wataru; Mori, Haruhide; Imai, Ryozo

doi:10.1016/j.plaphy.2018.12.017

Cited by 66 publications

(56 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Next to the DPF regulon, also the recently described TF OsERF83 is strongly upregulated by P. arrhenomanes infection. This TF is transiently induced by treating rice seedlings with SA or ethephon and more constantly triggered by MeJA (Tezuka et al, 2019). PR genes upregulated in rice lines overexpressing OsERF83 are also transcriptionally activated upon P. arrhenomanes infection ( Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

Jasmonate-Induced Defense Mechanisms in the Belowground Antagonistic Interaction Between Pythium arrhenomanes and Meloidogyne graminicola in Rice

et al. 2019

View full text Add to dashboard Cite

Next to their essential roles in plant growth and development, phytohormones play a central role in plant immunity against pathogens. In this study we studied the previously reported antagonism between the plant-pathogenic oomycete Pythium arrhenomanes and the root-knot nematode Meloidogyne graminicola, two root pathogens that co-occur in aerobic rice fields. In this manuscript, we investigated if the antagonism is related to imbalances in plant hormone levels, which could be involved in activation of plant defense. Hormone measurements and gene expression analyses showed that the jasmonate (JA) pathway is induced early upon P. arrhenomanes infection. Exogenous application of methyl-jasmonate (MeJA) on the plant confirmed that JA is needed for basal defense against both P. arrhenomanes and M. graminicola in rice. Whereas M. graminicola suppresses root JA levels to increase host susceptibility, Pythium inoculation boosts JA in a manner that prohibits JA repression by the nematode in double-inoculated plants. Exogenous MeJA supply phenocopied the defense-inducing capacity of Pythium against the root-knot nematode, whereas the antagonism was weakened in JA-insensitive mutants. Transcriptome analysis confirmed upregulation of JA biosynthesis and signaling genes upon P. arrhenomanes infection, and additionally revealed induction of genes involved in biosynthesis of diterpenoid phytoalexins, consistent with strong activation of the gene encoding the JA-inducible transcriptional regulator DITERPENOID PHYTOALEXIN FACTOR. Altogether, the here-reported data indicate an important role for JA-induced defense mechanisms in this antagonistic interaction. Next to that, our results provide evidence for induced expression of genes encoding ERF83, and related PR proteins, as well as auxin depletion in P. arrhenomanes infected rice roots, which potentially further contribute to the reduced nematode susceptibility seen in double-infected plants.

show abstract

Section: Resultsmentioning

confidence: 99%

Jasmonate-Induced Defense Mechanisms in the Belowground Antagonistic Interaction Between Pythium arrhenomanes and Meloidogyne graminicola in Rice

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Contrasting roles in disease resistance have been observed for different ERF genes. For example, OsERF922 and OsERF83 were found negatively and positively regulate rice resistance to M. oryzae in rice cultivars Zhonghua 17 and Yukihikari, respectively (Liu et al 2012 ; Tezuka et al 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Early Defenses in Rice against Fusarium fujikuroi

Cheng

Chen

Lai

et al. 2020

Rice

View full text Add to dashboard Cite

Background Bakanae is a seedborne disease caused by Fusarium fujikuroi. Rice seedlings emerging from infected seeds can show diverse symptoms such as elongated and slender stem and leaves, pale coloring, a large leaf angle, stunted growth and even death. Little is known about rice defense mechanisms at early stages of disease development. Results This study focused on investigating early defenses against F. fujikuroi in a susceptible cultivar, Zerawchanica karatals (ZK), and a resistant cultivar, Tainung 67 (TNG67). Quantitative PCR revealed that F. fujikuroi colonizes the root and stem but not leaf tissues. Illumina sequencing was conducted to analyze the stem transcriptomes of F. fujikuroi-inoculated and mock-inoculated ZK and TNG67 plants collected at 7 days post inoculation (dpi). More differentially expressed genes (DEGs) were identified in ZK (n = 169) than TNG67 (n = 118), and gene ontology terms related to transcription factor activity and phosphorylation were specifically enriched in ZK DEGs. Among the complex phytohormone biosynthesis and signaling pathways, only DEGs involved in the jasmonic acid (JA) signaling pathway were identified. Fourteen DEGs encoding pattern-recognition receptors, transcription factors, and JA signaling pathway components were validated by performing quantitative reverse transcription PCR analysis of individual plants. Significant repression of jasmonate ZIM-domain (JAZ) genes (OsJAZ9, OsJAZ10, and OsJAZ13) at 3 dpi and 7 dpi in both cultivars, indicated the activation of JA signaling during early interactions between rice and F. fujikuroi. Differential expression was not detected for salicylic acid marker genes encoding phenylalanine ammonia-lyase 1 and non-expressor of pathogenesis-related genes 1. Moreover, while MeJA did not affect the viability of F. fujikuroi, MeJA treatment of rice seeds (prior to or after inoculation) alleviated and delayed bakanae disease development in susceptible ZK. Conclusions Different from previous transcriptome studies, which analyzed the leaves of infected plants, this study provides insights into defense-related gene expression patterns in F. fujikuroi–colonized rice stem tissues. Twelve out of the 14 selected DEGs were for the first time shown to be associated with disease resistance, and JA-mediated resistance was identified as a crucial component of rice defense against F. fujikuroi. Detailed mechanisms underlying the JA-mediated bakanae resistance and the novel defense-related DEGs are worthy of further investigation.

show abstract

“…Function and Expression Pattern of AP2/ERF TFs under Biotic Stresses AP2/ERF TFs were associated with the regulation of disease resistance in plants. Overexpression of TaPIE1 [157], OsEREBP1 [158], Soly106 [159], GmERF113 [160] and OsERF83 [161] genes, in response to JA, SA or ET mediated signal transduction pathways, were believed to be effective against pathogen infection. HvRAF, a novel AP2/ERF TF in barley, had dual regulatory functions under biotic (R. solanacearum) and abiotic (salinity) stresses [162].…”

Section: Function and Expression Pattern Of Ap2/erf Tfs Under Abioticmentioning

confidence: 99%

Transcription Factors in Plant Stress Responses: Challenges and Potential for Sugarcane Improvement

Javed

Shabbir

Ali

et al. 2020

Plants

174

107

View full text Add to dashboard Cite

Increasing vulnerability of crops to a wide range of abiotic and biotic stresses can have a marked influence on the growth and yield of major crops, especially sugarcane (Saccharum spp.). In response to various stresses, plants have evolved a variety of complex defense systems of signal perception and transduction networks. Transcription factors (TFs) that are activated by different pathways of signal transduction and can directly or indirectly combine with cis-acting elements to modulate the transcription efficiency of target genes, which play key regulators for crop genetic improvement. Over the past decade, significant progresses have been made in deciphering the role of plant TFs as key regulators of environmental responses in particular important cereal crops; however, a limited amount of studies have focused on sugarcane. This review summarizes the potential functions of major TF families, such as WRKY, NAC, MYB and AP2/ERF, in regulating gene expression in the response of plants to abiotic and biotic stresses, which provides important clues for the engineering of stress-tolerant cultivars in sugarcane.

show abstract

The rice ethylene response factor OsERF83 positively regulates disease resistance to Magnaporthe oryzae

Cited by 66 publications

References 57 publications

Jasmonate-Induced Defense Mechanisms in the Belowground Antagonistic Interaction Between Pythium arrhenomanes and Meloidogyne graminicola in Rice

Jasmonate-Induced Defense Mechanisms in the Belowground Antagonistic Interaction Between Pythium arrhenomanes and Meloidogyne graminicola in Rice

Transcriptome Analysis of Early Defenses in Rice against Fusarium fujikuroi

Transcription Factors in Plant Stress Responses: Challenges and Potential for Sugarcane Improvement

Contact Info

Product

Resources

About