The role of complement in hydatid disease: In vitro studies

Kassis, Amin I.; Tanner, C. E.

doi:10.1016/0020-7519(76)90007-2

Cited by 66 publications

(21 citation statements)

References 17 publications

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“…These changes have also been observed in protoscoleces cultured in the presence of other "BZD" Pérez-Serrano et al 1994), i.e. praziquantel (PZ) (Thompson et al 1986;Rodríguez-Caabeiro et al 1989;Urrea-París et al 1999, 2000 ivermectin ) and a bio-substance such as complement (Kassis and Tanner 1986). Pérez-Serrano et al (1994) assumed that loss of rostellar hooks and formation of blebs are "stress responses" brought about in protoscoleces by any harmful condition.…”

Section: Discussionmentioning

confidence: 84%

In vitro effects of flubendazole on Echinococcus granulosus protoscoleces

et al. 2005

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The aim of the present work was to determine the in vitro protoscolicidal effect of flubendazole (FLBZ) against Echinococcus granulosus. Protoscoleces of E. granulosus were incubated with FLBZ at concentrations of 10, 5 and 1 microg/ml. The first signs of FLBZ-induced damage were observed 3 days post-incubation. A clear protoscolicidal effect, reducing the vitality of protoscoleces to 35.6+/-0.7%, was observed after 18 days of incubation. After 25 days of FLBZ incubation (5 microg/ml), the percentage of vital protoscoleces was 13.9+/-5.9%. Protoscolex mortality was 100% (10 and 1 microg/ml) and 0.7+/-0.7% (5 microg/ml) after FLBZ incubation for 30 days. Results of vitality tests were consistent with the tissue damage observed at the ultrastructural level. The primary site of damage was the tegument of the parasite. The morphological changes included contraction of the soma region, formation of blebs on the tegument, rostellar disorganization, loss of hooks and destruction of microtriches. The data reported in this article demonstrate a clear in vitro effect of FLBZ against E. granulosus protoscoleces.

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Section: Discussionmentioning

confidence: 84%

In vitro effects of flubendazole on Echinococcus granulosus protoscoleces

et al. 2005

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“…Lysis in both immune and normal serum is antibody dependent and complement mediated (116). Protoscoleces of E. multilocularis and E. granulosus are lysed by fresh serum of many different species of mammals (121,152). The presence of Echinococcus cysts appears to deplete host complement (121,150,151).…”

Section: Inhibition Of Cyst Growthmentioning

confidence: 99%

Concepts in Immunology and Diagnosis of Hydatid Disease

2003

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Echinococcosis is a cosmopolitan zoonosis caused by adult or larval stages of cestodes belonging to the genus Echinococcus (family Taeniidae). The two major species of medical and public health importance are Echinococcus granulosus and E. multilocularis, which cause cystic echinococcosis (CE) and alveolar echinococcosis (AE), respectively. Both CE and AE are both serious diseases, the latter especially so, with a high fatality rate and poor prognosis if managed inappropriately. This review discusses new concepts and approaches in the immunology and diagnosis of CE, but comparative reference has also been made to AE infection and to earlier pivotal studies of both diseases. The review considers immunity to infection in the intermediate and definitive hosts, innate resistance, evasion of the immune system, and vaccination of intermediate and definitive hosts, and it particularly emphasizes procedures for diagnosis of CE and AE, including the value of immunodiagnostic approaches. There is also discussion of the new advances in recombinant and related DNA technologies, especially application of PCR, that are providing powerful tools in the fields of vaccinology and molecular diagnosis of echinococcosis

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“…Several workers have sho\vn that hydatid cyst components, as well as materials from the larval stages of other taeniid cestodes, contain anticomplementary substances which spontaneously activate complement perhaps via the alternate pathbvay (Herd 1976;Hammerberg ef a/ 1976Hammerberg ef a/ . 1977Kassis and Tanner 1976;Rickard et a/ 1977a). Hammerberg cf a/ ( 1 977) showed that pronounced vascular changes occurred in the skin of humans follobving intradermal inoculation of complement-activating fractions of hgdatid cyst fluid.…”

Section: Lntradermal (Casoni) Testmentioning

confidence: 99%

The Immunological Diagnosis of Hydatid Disease

Rickard

1979

Aust Veterinary J

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SUMMARY: The tests that have been most commonly employed for the diagnosis of hydatid disease in Australia include the intradermal test (ID or Casoni test), complement fixation test (CFT), indirect haemagglutination (IHA) test, latex agglutination (LA) test, indirect fluorescent antibody test (IFAT) and the immunoelectrophoresis test (IEP). More recently developed tests are the enzyme‐linked immunosorbent assay (ELISA), radioimmunoassay (RIA), and countercurrent immunoelectrophoresis (CIE). This paper outlines the uses for immunological diagnosis of hydatid disease and the criteria for determining the test of choice in a particular circumstance. The principles of each test, and their usefulness in terms of the criteria set down is discussed. At the present time, for pre‐treatment diagnosis IHA and LA appear to be the best suited for screening purposes while IEP is the most specific diagnostic test. For postoperative follow up IEP and the CFT are both useful.

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The role of complement in hydatid disease: In vitro studies

Cited by 66 publications

References 17 publications

In vitro effects of flubendazole on Echinococcus granulosus protoscoleces

In vitro effects of flubendazole on Echinococcus granulosus protoscoleces

Concepts in Immunology and Diagnosis of Hydatid Disease

The Immunological Diagnosis of Hydatid Disease

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