The role of electrophoresis in disease biomarker discovery

Issaq, Haleem J.; Veenstra, Timothy D.

doi:10.1002/elps.200600834

Cited by 61 publications

(43 citation statements)

References 53 publications

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“…Depleted sera are then either separated by 2D gel electrophoresis (DIGE) that allows comparative analysis of three different samples in a single gel, or by tryptic digests of serum proteome fractions that have been labeled with so-called isobaric tags (iTRAQ technology), permitting parallel analysis of up to eight serum samples. [91][92][93] Identification of the in gel trypsin digested proteins (DIGE) or of the tryptic iTRAQ peptides is done by MS. The power of these technologies lies in a quantitative comparison of an identified peptide or protein in three (DIGE) or eight (iTRAQ) serum samples.…”

Section: Noninvasive Means To Assess Liver Fibrosis and Fibrogenesismentioning

confidence: 99%

Targeting Liver Fibrosis

2009

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We have made striking progress in our understanding of the biochemistry and cell biology that underlies liver fibrosis and cirrhosis, including the development of strategies and agents to prevent and reverse fibrosis. However, translation of this knowledge into clinical practice has been hampered by (1) the limitation of many in vitro and in vivo models to confirm mechanisms and to test antifibrotic agents, and (2) the lack of sensitive methodologies to quantify the degree of liver fibrosis and the dynamics of fibrosis progression or reversal in patients. Furthermore, whereas cirrhosis and subsequent decompensation are accepted hard clinical endpoints, fibrosis and fibrosis progression alone are merely plausible surrogates for future clinical deterioration. In this review we focus on an optimized strategy for preclinical antifibrotic drug development and highlight the current and future techniques that permit noninvasive assessment and quantification of liver fibrosis and fibrogenesis. The availability of such noninvasive methodologies will serve as the pacemaker for the clinical development and validation of potent antifibrotic agents. (HEPATOLOGY 2009;50:1294-1306

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Section: Noninvasive Means To Assess Liver Fibrosis and Fibrogenesismentioning

confidence: 99%

Targeting Liver Fibrosis

2009

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“…Throughout the last decade, CE has been established as a reliable tool in biomarker discovery [5] and anticancer research, especially in the analysis of antitumor drugs based on transition metal complexes [6,7]. It has been applied to investigate the stability of novel compounds but also the interactions of drugs with biomolecules, such as human serum proteins [8][9][10][11][12][13][14][15] and nucleotides [16][17][18][19][20][21], elucidating possible metabolic and reaction pathways of the drug candidates [6,7,16], notably always working under simulated physiological conditions with regard to pH, temperature, aqueous solutions, and/or buffer composition.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis hyphenated to inductively coupled plasma‐mass spectrometry: A novel approach for the analysis of anticancer metallodrugs in human serum and plasma

et al. 2008

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Capillary electrophoresis hyphenated to inductively coupled plasma-mass spectrometry: A novel approach for the analysis of anticancer metallodrugs in human serum and plasmaThe development of metal-based chemotherapeutics lacks methods which are capable of providing early indication on the potential of new metal complexes as future anticancer drugs. Since most of these compounds are administered intravenously, serum proteins are the first available biological binding partners in the bloodstream. For platinum-based anticancer drugs the interaction with serum proteins is regarded as an important contribution to the side effects accompanying chemotherapy. In contrast, newly developed ruthenium compounds are thought to be transported into the tumor in a protein-bound form. In here, the application of CE hyphenated to inductively coupled plasma (ICP)-MS, applying Polybrene-coated capillaries, is demonstrated for studying the interaction of indazolium [trans-tetrachlorobis(1H-indazole)ruthenate(III)] (KP1019) with HSA and transferrin, which are important transport proteins. Furthermore, the applicability of the method to human serum and plasma and, more importantly, to real-world patient samples was proven. KP1019 was found to bind to a high degree to HSA both in serum, plasma and the patient samples. Only minor fractions of ruthenium were found attached to other proteins.

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“…Biomarkers are substances found in biological fluids such as saliva 43 , which facilitates sample collection, and contains elements that reflect both the local and systemic condition of the patients 44 .…”

Section: Salivary Biomarkersmentioning

confidence: 99%

Dental perspective on Sjögren’s syndrome: literature review

et al. 2015

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Abstract:The human body releases around 500-600mL of saliva daily, however when values of unstimulated whole saliva range from 0.1 to 0.2mL/ min, there is a condition called Hyposalivation or hyposialia. Hyposalia is characterized by a large number of systemic conditions, including Sjögren's syndrome, a chronic autoimmune disease that affects between 0.1 and 3% of the world population and is characterized by exocrinopathy of the salivary glands leading to glandular hypofunction and thus decreasing the normal salivary flow. Saliva is part of innate immunity, when there is a decrease in protein secretion, numerous oral manifestations occur such as dental caries, candidiasis, gingival disease, angular cheilitis, lymphomas of the salivary glands, dysphagia, erythematous and fissured tongue, among others. Currently there is no defined dental treatment, however there are alternative treatments by sialogogues and salivary substitutes, plus non-pharmacological therapies, which seek to maintain the ecology and oral conditions stable, in addition to preventive and restorative dental treatment for lesions already established as a consequence of the disease. The aim of this study is to conduct a literature review on the characteristics, classification, oral manifestations and dental management of Sjögren's syndrome.

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The role of electrophoresis in disease biomarker discovery

Cited by 61 publications

References 53 publications

Targeting Liver Fibrosis

Targeting Liver Fibrosis

Capillary electrophoresis hyphenated to inductively coupled plasma‐mass spectrometry: A novel approach for the analysis of anticancer metallodrugs in human serum and plasma

Dental perspective on Sjögren’s syndrome: literature review

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