The Role of Glutathione Metabolism in Tolerance of Tobacco BY-2 Suspension Cells to Microcystin-RR

Abstract

This study was undertaken to investigate the role of the glutathione-involved detoxifying mechanism in defending the tobacco BY-2 suspension cells against microcystin-RR (MC-RR). Analysis showed that exposure of the cells to different concentrations of MC-RR (0.1, 1 and 10 lg/mL) for 0-6 days resulted in a time and concentration-dependent decrease in cell viability and increase in reactive oxygen species (ROS) content. Reduced glutathione (GSH) and total glutathione (tGSH) content as well as glutathione reductase (GR), glutathione peroxidase (GPX) and glutathione-S-transferase (GST) activities significantly increased after 3-4 days exposure in the highest two concentration treated groups, while decreased until reaching the control values except for GPX at day 6. Oxidized glutathione (GSSG) content markedly increased compared with control in high concentration MC-RR treated group after 6 days exposure. The GSH/GSSG ratio was much higher than control in 10 lg/mL MC-RR treated group at day 4, but after 6 days exposure, the ratios in all treated groups were lower than that of the control group.

Lipid peroxidation and antioxidant responses in zebrafish brain induced by Aphanizomenon flos-aquae DC-1 aphantoxins

“…For example, GSH, as an essential enzyme substrate, may react with GPx to protect against oxidative damage from endogenous ROS, which is supported by previous results (Huang et al, 2008). Thus, in the present study, the obvious increase in GPx activity after toxin exposure indicated that the rapid depletion of GSH participated in the elimination of ROS (Žegura et al, 2006).…”

Antioxidative responses of Elodea nuttallii (Planch.) H. St. John to short-term iron exposure

“…Among them, glutathione-S-transferase (GST) plays a critical role in defending the organism against reactive electrophiles by removing them through conjugation with GSH [23]. In addition, GST is ubiquitous multifunctional proteins involved in the detoxification of toxicant, which catalyses the conjugation of GSH to various electrophilic substrates [15]. In our experiment, the elevation of GST suggested that the detoxification appears to proceed via glutathione conjugation by GST.…”

Effects of Fertilizer-Urea on Growth, Photosynthetic Activity and Microcystins Production of Microcystis aeruginosa Isolated from Dianchi Lake

“…The maximum quantum yields of PSII (Fv/Fm), photosynthetic efficiency (a), saturation irradiance (I k ) and maximum electron transport rate (ETR max ) were obtained in Report-windows of Phyto-PAM. The extraction and measurement of MCs from the M. aeruginosa cells were according to the method of Huang et al (2008).…”