The Role of KH-Type Splicing Regulatory Protein (KSRP) for Immune Functions and Tumorigenesis

Palzer, Kim-Alicia; Bolduan, Vanessa; Käfer, Rudolf; Kleinert, Hartmut; Bros, Matthias; Pautz, Andrea

doi:10.3390/cells11091482

Cited by 19 publications

(6 citation statements)

References 137 publications

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“…In colorectal cancer, enhanced expression of KSRP was found in tumour tissue and was associated with worse overall survival. KSRP seemed to drive epithelial cell proliferation in primary and metastatic cells through control of cell cycle progression and promoted angiogenesis by enhancing vascular endothelial growth factor 46 .…”

Section: Discussionmentioning

confidence: 99%

ENOX1, CCDC122ANDLACC1ROLE IN PROGRESSION OF PROSTATE CANCER

Ongaba

2023

Preprint

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Prostate cancer (PCa) continues to trend among top 3 cancers that kill men over 20 years in the United Kingdom and worldwide despite extensive research and resources directed towards its treatment and prevention. In the application of a hallmark of survival mechanisms by the cancer, our study used mRNA seq data to identify genes that are increasingly mutated with progressing PCa from a cohort of 491 PCa patients. We found thatENOX1, CCDC122andLACC1deep deletion was positively associated with increasing age of diagnosis. Pathway analysis of enriched genes, following their deep deletion identified estrogen biosynthesis, KSRP signalling, omega 3 and 6 fatty acid biosynthesis and, Rap1 signalling as the top 5 enriched pathways. Previous individual and combined role of these genes in PCa progression was not fully established but thanks to this study, these pathways could be druggable targets in PCa patients with these gene deep deletions.

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Section: Discussionmentioning

confidence: 99%

ENOX1, CCDC122ANDLACC1ROLE IN PROGRESSION OF PROSTATE CANCER

Ongaba

2023

Preprint

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“…In addition, other four-type motifs were identified in the OfCCCH protein sequences, including ANK, KH, PRM, and WD40 motifs. All of these motifs play important roles in diverse molecular processes [ 21 ], splicing and transcriptional regulation [ 22 ], growth processes [ 23 ], and stress response [ 24 ]. Many CREs associated with plant hormones were predicted in the OfCCCH promoters in O. fragrans ( Figure 6 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis of CCCH Family in Roles of Flower Opening and Abiotic Stress in Osmanthus fragrans

Ye¹,

Cao²,

Lei³

et al. 2022

IJMS

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CCCH is a zinc finger family with a typical CCCH-type motif which performs a variety of roles in plant growth and development and responses to environmental stressors. However, the information about this family has not been reported for Osmanthus fragrans. In this study, a total of 66 CCCH predicted genes were identified from the O. fragrans genome, the majority of which had multiple CCCH motifs. The 66 OfCCCHs were found to be unevenly distributed on 21 chromosomes and were clustered into nine groups based on their phylogenetic analysis. In each group, the gene structure and domain makeup were comparatively conserved. The expression profiles of the OfCCCH genes were examined in various tissues, the flower-opening processes, and under various abiotic stresses using transcriptome sequencing and qRT-PCR (quantitative real-time PCR). The results demonstrated the widespread expression of OfCCCHs in various tissues, the differential expression of 22 OfCCCHs during flower-opening stages, and the identification of 4, 5, and 13 OfCCCHs after ABA, salt, and drought stress treatment, respectively. Furthermore, characterization of the representative OfCCCHs (OfCCCH8, 23, 27, and 36) revealed that they were all localized in the nucleus and that the majority of them had transcriptional activation in the yeast system. Our research offers the first thorough examination of the OfCCCH family and lays the groundwork for future investigations regarding the functions of CCCH genes in O. fragrans.

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“…The different properties of KSRP and TsPUF enable us to speculate on the pathway of RNA secretion from T. spiralis MSL. KSRP in humans is able to bind to a variety of RNA targets [32], including miRNA precursors via an interaction with the unpaired region of the stem-loop [29]. This interaction is proposed to underpin the requirement for KSRP in processing specific miRNAs [29].…”

Section: Insights Into the Mechanism Of Rna Secretion By T Spiralismentioning

confidence: 99%

Identification of proteins that bind extracellular microRNAs secreted by the parasitic nematode Trichinella spiralis

Sarkies

Selkirk

Brown

2023

Preprint

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Small non-coding RNAs such as microRNAs (miRNAs) are conserved across eukaryotes and play key roles in regulating gene expression. In many organisms, miRNAs are also secreted from cells, often encased within vesicles such as exosomes and sometimes extravesicular. The mechanisms of miRNA secretion, how they are stabilised outside of cells and their functional importance are poorly understood. Recently we characterised the parasitic nematode Trichinella spiralis as a model to study miRNA secretion. T. spiralis larvae secrete abundant miRNAs which are largely extravesicular. Here, we investigated how T. spiralis miRNAs might remain stable outside of cells. Using proteomics, we identified two RNA binding proteins secreted by T. spiralis larvae and characterised their RNA binding properties. One, a homologue of the known RNA binding protein KSRP, binds miRNA in a selective and sequence-specific fashion. Another protein, which is likely a novel RNA binding protein, binds non-selectively to miRNA. Our results suggest a possible mechanism for miRNA secretion by T. spiralis and may have relevance for understanding the biology of extracellular miRNA more widely.

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The Role of KH-Type Splicing Regulatory Protein (KSRP) for Immune Functions and Tumorigenesis

Cited by 19 publications

References 137 publications

ENOX1, CCDC122ANDLACC1ROLE IN PROGRESSION OF PROSTATE CANCER

ENOX1, CCDC122ANDLACC1ROLE IN PROGRESSION OF PROSTATE CANCER

Comparative Transcriptome Analysis of CCCH Family in Roles of Flower Opening and Abiotic Stress in Osmanthus fragrans

Identification of proteins that bind extracellular microRNAs secreted by the parasitic nematode Trichinella spiralis

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