The Role of miR-150 in Stress-Induced Anxiety-Like Behavior in Mice

Zhang, Wenjuan; Cao, Wenzhi; Huang, Yanqing; Cui, Yanhui; Tu, Bo-Xuan; Wang, Lai-Fa; Zou, Guang-Jing; Liu, Yu; Hu, Zhao‐Lan; Hu, Rong; Xing, Xiaowei; Li, Fang

doi:10.1007/s12640-018-9943-x

Cited by 23 publications

(6 citation statements)

References 74 publications

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“…Moreover, miRNAs reportedly participate in synaptic development, dendritic protein synthesis, axon guidance and neuronal formation by regulating the translation process of mRNAs [ 10–13 ]. In addition, miRNAs have been associated with stress-induced mood disorders [ 14 , 15 ]. Accumulating evidence indicates that miRNAs play important roles in regulating depression, including miR-323a, miR-207 and miR-34a [ 16–18 ].…”

Section: Introductionmentioning

confidence: 99%

Expression and role of microRNA-212/nuclear factor I-A in depressive mice

Liang¹,

Wang²,

Liu³

et al. 2021

Bioengineered

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Depression is characterized by persistent depressed mood and cognitive dysfunction, severely impacting human health. In the present study, we aimed to explore the role and mechanism of microRNA (miR)-212 in depression in vivo . Chronic unpredictable mild stress (CUMS) mice were established, and depression-like behaviors were confirmed using the forced swimming test (FST), sucrose preference test (SPT), and the tail suspension test (TST). Next, the expression of miR-212 and its potential target, i.e., nuclear factor I-A (NFIA), was verified using quantitative reverse transcription (qRT)-PCR analysis and Western blotting in CUMS mice. The effects of miR-212 and NFIA on depression-like behaviors, inflammatory response, and neuronal apoptosis were examined using FST, TST, SPT, enzyme-linked immunosorbent assay (ELISA) assay, and flow cytometry analysis. Finally, the relationship between miR-212 and NFIA was examined using a dual-luciferase reporter assay. Based on our findings, miR-212 was significantly upregulated, while NFIA was downregulated in CUMS mice. miR-212 overexpression could suppress the CUMS-induced weight loss, immobility time in FST and TST, and increased hippocampal neuronal apoptosis and pro-inflammatory cytokines levels. In addition, NFIA upregulation could partially reverse the effects of miR-212 mimic in CUMS mice. Accordingly, miR-212 could ameliorate CUMS-induced depression-like behavior in mice by targeting NFIA, indicating its protective role in depression.

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Section: Introductionmentioning

confidence: 99%

Expression and role of microRNA-212/nuclear factor I-A in depressive mice

Liang¹,

Wang²,

Liu³

et al. 2021

Bioengineered

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“…A study on adult transgenic mice has shown that miR-150 knockout causes anxiety-like behavior in mice according to an open-field test and an elevated plus-maze test. Furthermore, decreased dendrite lengths, dendrite branching, and dendrite spines have been observed in miR-150 knockout mice compared to wild-type mice, and there is a change in the expression of glutamatergic receptors [ 26 ].…”

Section: Resultsmentioning

confidence: 99%

MAPK Is a Mutual Pathway Targeted by Anxiety-Related miRNAs, and E2F5 Is a Putative Target for Anxiolytic miRNAs

et al. 2023

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Anxiety-related disorders (ARDs) are chronic neuropsychological diseases and the sixth leading cause of disability in the world. As dysregulation of microRNAs (miRs) are observed in the pathological course of neuropsychiatric disorders, the present study aimed to introduce miRs that underlie anxiety processing in the brain. First, we collected the experimentally confirmed anxiety-related miRNAs (ARmiRs), predicted their target transcripts, and introduced critical cellular pathways with key commune hub genes. As a result, we have found nine anxiolytic and ten anxiogenic ARmiRs. The anxiolytic miRs frequently target the mRNA of Acyl-CoA synthetase long-chain family member 4 (Acsl4), AFF4-AF4/FMR2 family member 4 (Aff4), and Krüppel like transcription factor 4 (Klf4) genes, where miR-34b-5p and miR-34c-5p interact with all of them. Moreover, the anxiogenic miRs frequently target the mRNA of nine genes; among them, only two miR (miR-142-5p and miR-218-5p) have no interaction with the mRNA of trinucleotide repeat-containing adaptor 6B (Tnrc6b), and miR-124-3p interacts with all of them where MAPK is the main signaling pathway affected by both anxiolytic and anxiogenic miR. In addition, the anxiolytic miR commonly target E2F transcription factor 5 (E2F5) in the TGF-β signaling pathway, and the anxiogenic miR commonly target Ataxin 1 (Atxn1), WASP-like actin nucleation promoting factor (Wasl), and Solute Carrier Family 17 Member 6 (Slc17a6) genes in the notch signaling, adherence junction, and synaptic vesicle cycle pathways, respectively. Taken together, we conclude that the most important anxiolytic (miR-34c, Let-7d, and miR-17) and anxiogenic (miR-19b, miR-92a, and 218) miR, as hub epigenetic modulators, potentially influence the pathophysiology of anxiety, primarily via interaction with the MAPK signaling pathway. Moreover, the role of E2F5 as a novel putative target for anxiolytic miRNAs in ARDs disorders deserves further exploration.

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“…A related study also showed acute foot-shock stress paradigm was thought to induce depressive-like behavior in mice (W. J. Zhang et al, 2019). Restraint has been widely characterized as an acute stressor as it is a simple experimental procedure with high reproducibility (Malisch et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Scutellarin ameliorates the stress‐induced anxiety‐like behaviors in mice by regulating neurotransmitters

Guo

Zhou

et al. 2021

Phytotherapy Research

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Anxiety disorders are a common frequently psychiatric symptom in patients that lead to disruption of daily life. Scutellarin (Scu) is the main component of Erigeron breviscapus, which has been used as a neuroprotective agent against glutamateinduced excitotoxicity. However, the potential effect of Scu on the stress-related neuropsychological disorders has not been clarified. In this study, Anxiety-like behavior was induced by acute restraint stress in mice. Scu were injected intraperitoneally (twice daily, 3 days). Results showed that Scu exhibited good protective activity on mice by decreasing transmitter release levels. Restraint stress caused significant anxiety like behavior in mice. Treatment of Scu could significantly improve the moving time of open arms in Elevated Plus Maze and central time on open field test. Scu treatment suppressed action potential firing frequency, restored excessive presynaptic quantal release, and down-regulated glutamatergic receptor expression levels in the prefrontal cortex (PFC) of stressed mice. GABA A Rα1 and GABA A γ 2 expression in the brain PFC tissues of mice were nearly abrogated by Scu treatment. In stressinduced anxiety mice, stress can increase the frequency of mini excitatory postsynaptic currents (mEPSC), which can be reversed by Scu treatment. Therefore, Scu has a potent anxiolytic activity and may be valuable for the treatment of stress-induced anxiety disorders.

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The Role of miR-150 in Stress-Induced Anxiety-Like Behavior in Mice

Cited by 23 publications

References 74 publications

Expression and role of microRNA-212/nuclear factor I-A in depressive mice

Expression and role of microRNA-212/nuclear factor I-A in depressive mice

MAPK Is a Mutual Pathway Targeted by Anxiety-Related miRNAs, and E2F5 Is a Putative Target for Anxiolytic miRNAs

Scutellarin ameliorates the stress‐induced anxiety‐like behaviors in mice by regulating neurotransmitters

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