The Role of miRNA in Differentiation, Cell Proliferation, and Pathogenesis of Poultry Diseases

Yakovlev, A. F.

doi:10.1134/s1062360419030081

Cited by 5 publications

(6 citation statements)

References 93 publications

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“…The miRNA expression levels in circulation and in tissue are thought to be clinical biomarkers for the diagnosis and prognosis of atherosclerosis [ 7 ]. Numerous studies have demonstrated that miRNAs are involved in a variety of basic biological procedure, such as cell development, growth, proliferation, differentiation, and apoptosis [ 8 ]. Emerging evidence suggests that miRNAs play a prominent role in regulating the functions of VSMCs during atherogenesis [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

microRNA-491-5p protects against atherosclerosis by targeting matrix metallopeptidase-9

Wang

et al. 2020

Open Medicine

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AbstractAbnormal proliferation and migration of vascular smooth muscle cells (VSMCs) are critical processes that are involved in atherosclerosis. The aim of this study was to explore the role of microRNA-491-5p (miR-491-5p) in the progression of atherosclerosis by regulating the growth and migration of VSMCs. In this study, we showed that the expression of miR-491-5p was downregulated in the atherosclerotic plaque tissues and plasma samples of the patients with atherosclerosis. The bioinformatic analysis and dual-luciferase reporter assay identified that matrix metallopeptidase-9 (MMP-9) was a target gene of miR-491-5p. The results showed a significant upregulation of MMP-9 in the atherosclerotic plaque tissues and plasma samples. Subsequently, the results also showed that downregulation of miR-491-5p significantly promoted the proliferation and migration of VSMCs and inhibited the apoptosis in VSMCs. Furthermore, we detected the effects of miR-491-5p mimic on the growth and migration of VSMCs, and the results illustrated that miR-491-5p mimic could inhibit the proliferation and migration of VSMCs and promote the apoptosis of VSMCs. Notably, MMP-9 plasmid could reverse all the effects of miR-491-5p mimic on VSMCs. Collectively, our study provides the first evidence that miR-491-5p inhibited the growth and migration of VSMCs by targeting MMP-9, which might provide new biomarkers and potential therapeutic targets for atherosclerosis treatment.

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Section: Introductionmentioning

confidence: 99%

microRNA-491-5p protects against atherosclerosis by targeting matrix metallopeptidase-9

Wang

et al. 2020

Open Medicine

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“…MiRNA is a class of small, non-coding endogenous RNAs that play an essential role in gene regulation. The dysregulation of miRNA expressions has been linked to various human diseases, including cancer. − As a result, miRNA has become a promising biomarker for early diagnosis and prognosis. , However, the challenge of miRNA detection is heightened by their small size and limited abundance, along with the considerable similarity in their sequences. , At present, the quantitative reverse transcription real-time polymerase chain reaction (qRT-PCR) is the gold standard for miRNA detections. , However, this approach has certain limitations, such as the need for exogenous enzymes, complex preprocessing steps, and difficulties with short miRNA design primers, which make it unsuitable for intracellular applications. − Given the limitations of the current methods, there is a significant need to develop a miRNA detection method that is not only sensitive, reliable, and accurate but also well-suited for intracellular applications.…”

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confidence: 99%

“…1−3 As a result, miRNA has become a promising biomarker for early diagnosis and prognosis. 4,5 However, the challenge of miRNA detection is heightened by their small size and limited abundance, along with the considerable similarity in their sequences. 6,7 At present, the quantitative reverse transcription real-time polymerase chain reaction (qRT-PCR) is the gold standard for miRNA detections.…”

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confidence: 99%

Three-Dimensional CHA-HCR System Using DNA Nanospheres for Sensitive and Rapid Imaging of miRNA in Live Cells and Tissues

Liang

Qin

et al. 2023

Anal. Chem.

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Isothermal, enzyme-free amplification techniques, such as the hybridization chain reaction (HCR) and catalytic hairpin assembly (CHA), have gained increasing attention for miRNA analysis. However, current methodological challenges, including slow kinetics, low amplification efficiency, difficulties in efficient cellular internalization of DNA probes, and concerns regarding the intracellular stability of nucleic acids, need to be addressed. To this end, we propose a novel strategy for sensitive miRNA detection based on a three-dimensional (3D) CHA-HCR system. This system comprises two DNA nanospheres, named DS-13 and DS-24, which are functionalized with CHA and HCR hairpins. Target miR-21 initiates CHA between the two nanospheres, thereby activating downstream HCR and bringing cyanine 3 (Cy3) and cyanine 5 (Cy5) into proximity. The 3D CHA-HCR process leads to the formation of large DNA aggregates and the generation of fluorescence resonance energy transfer signals. In this strategy, the employment of a cascaded reaction and spatial confinement effect improve sensitivity and kinetics, while the use of DNA nanocarriers facilitates cellular delivery and protects nucleic acid probes. The experimental results in vitro, in living cells, and in clinical tissue samples demonstrated the desirable sensing performance. Collectively, this approach holds promise as a valuable tool for cancer diagnosis and biomedical research.

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“…As a kind of small non protein encoded ribonucleic acids composed of 18–25 nucleotides in animal and plant cells, microRNAs (miRNAs) have been reported to play significant roles in different disease states, including human cancer. − Particularly, the tumor-associated miRNAs existing in various cancer cells are relevant biomarkers in providing a wealth of information on cancer diagnosis and treatment. Analyzing the expression levels of tumor-related miRNAs in living cells can greatly help to understand the cell division and synergistic regulation mechanism of miRNAs in tumor growth. , Various techniques, such as quantitative reverse transcription polymerase chain reaction (qRT-PCR) and microarrays technology, are applied in miRNAs diagnosis. , However, they are confronted with the challenges over the destruction to the cellular activity and the cumbersome steps.…”

mentioning

confidence: 99%

“…Analyzing the expression levels of tumor-related miRNAs in living cells can greatly help to understand the cell division and synergistic regulation mechanism of miRNAs in tumor growth. 4,5 Various techniques, such as quantitative reverse transcription polymerase chain reaction (qRT-PCR) and microarrays technology, are applied in miRNAs diagnosis. 6,7 However, they are confronted with the challenges over the destruction to the cellular activity and the cumbersome steps.…”

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confidence: 99%

Encoding DNA Frameworks for Amplified Multiplexed Imaging of Intracellular microRNAs

et al. 2021

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Real-time imaging of multiple low-abundance microRNAs (miRNAs) simultaneously in living cells with high sensitivity is of vital importance for accurate cancer clinical diagnosis and prognosis studies. Maintaining stability of nanoprobes resistant to enzyme degradation and enabling effective signal amplification is highly needed for in vivo imaging studies. Herein, a rationally designed one-pot assembled multicolor tetrahedral DNA frameworks (TDFs) by encoding multicomponent nucleic acid enzymes (MNAzymes) was developed for signal-amplified multiple miRNAs imaging in living cells with high sensitivity and selectivity. TDFs could enter cells via self-delivery with good biocompatibility and stability. Two kinds of MNAzymes specific for miRNA-21 and miRNA-155 with fluorescein labeling were encoded in the structure of TDFs respectively through one-step thermal annealing. In the intracellular environment, the TDFs could be specifically bound with its specific miRNA target and form an active DNAzyme structure. The cleavage of the active site would trigger the release of target miRNA and circular fluorescence signal amplification, which enabled accurate diagnosis on miRNA identifications of different cell lines with high sensitivity. Meanwhile, with the specific AS1411 aptamer targeting for nucleolin overexpressed on the surface of the carcinoma cells, this well-designed TDFs nanoprobe exhibited good discrimination between cancer cells and normal cells. The strategy provides an efficient tool for understanding the biological function of miRNAs in cancer pathogenesis and therapeutic applications.

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The Role of miRNA in Differentiation, Cell Proliferation, and Pathogenesis of Poultry Diseases

Cited by 5 publications

References 93 publications

microRNA-491-5p protects against atherosclerosis by targeting matrix metallopeptidase-9

microRNA-491-5p protects against atherosclerosis by targeting matrix metallopeptidase-9

Three-Dimensional CHA-HCR System Using DNA Nanospheres for Sensitive and Rapid Imaging of miRNA in Live Cells and Tissues

Encoding DNA Frameworks for Amplified Multiplexed Imaging of Intracellular microRNAs

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