The role of nuclear morphometry in prediction of prognosis for rhabdomyosarcoma in children

Abstract

Nuclear morphometry is a useful tool in the assessment of children with RMS. Additionally, certain morphometric parameters could be easily applied in a selection of patients with good prognosis.

Detection and Quantification of Nuclear Morphology Changes in Apoptotic Cells by Fluorescence Microscopy and Subsequent Analysis of Visualized Fluorescent Signals

“…By utilizing a BZ-9000 fluorescent microscope system with BZ II Analyzer software, the nucleus size of LNCaP and MDA-MB-231 cancer cells was determined with mean nuclear area values of ~150 μm 2 , which is in accordance with literature data indicating that the eukaryotic nucleus size primarily varies in the range from 50 to 150 μm 2 (17,18). Using a computerized approach for the determination of nuclear parameters (area, perimeter, major and minor axis, brightness of nuclear DAPI signal), which were additionally confirmed by TUNEL assay, this study demonstrates capability of fluorescence microscopy to significantly detect apoptosis-specific modulations of nuclear morphology and, thus, provides an alternative tool for experimental apoptosis quantification in cell culture models.…”

Análise da morfometria nuclear: descrição da metodologia e o papel dos softwares de edição de imagem

“…Parâmetros morfométricos também foram utilizados no estudo de neoplasias ósseas e de partes moles, como nos trabalhos de Oda et al (23) , em 1995, avaliando tumor neuroectodérmico primitivo (PNET)/sarcomas de Ewing ósseos, e de Kazanowska et al (17) , em 2004, que estudaram rabdomiossarcomas humanos.…”

“…Kazanowska et al (17) concluíram que a morfometria nuclear pode auxiliar na subclassificação desses tumores, e que parâ-metros como coeficiente de arredondamento (CA) nuclear e tendência do núcleo a ser elíptico podem ser utilizados como fatores prognósticos. Pacientes com CA < 0,6 e com mais de 10% de células fusiformes tiveram melhor prognóstico por apresentarem, na visão dos autores, células mais bem diferenciadas, tendendo a ser tumores menos agressivos.…”

Quantitative Pathology: Historical Background, Clinical Research and Application of Nuclear Morphometry and DNA Image Cytometry

“…Until now, however, few studies have used morphometric measurements to determine the nuclear size and shape profiles in normal and neoplastic colorectal tissues (67). Not unexpectedly, the nuclear size is usually larger and its shape is more often irregular in cancer cells (68,69).…”