The role of vitronectin in the attachment and spatial distribution of bone-derived cells on materials with patterned surface chemistry

Thomas, Carson H.; McFarland, Clive D.; Jenkins, Michelle; Rezania, Alireza; Steele, John G.; Healy, Kevin E.

doi:10.1002/(sici)1097-4636(199710)37:1<81::aid-jbm10>3.0.co;2-t

Cited by 159 publications

(95 citation statements)

References 35 publications

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“…Lom et al 116,269 used patterns of aminosilane (N-(2-aminoethyl)-3-aminopropyl-trimethoxysilane [EDS]) and alkylsilane (dimethyldichlorosilane [DMS]) SAMs to spatially control the attachment of osteosarcoma cells; bone-derived cells required the presence of serum or vitronectin to organize in compliance with the lithographically defined surface chemistry. 270 Cells were randomly distributed over the EDS/DMS surface at the time of plating but reorganized on the EDS regions within 30 minutes. Using a radial flow apparatus, they found that within 20 minutes, the strength of adhesion was significantly larger on EDS and clean surfaces than on DMS surfaces.…”

Section: Vg Bone Cell Biology On a Chipmentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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The ability to culture cells in vitro has revolutionized hypothesis testing in basic cell and molecular biology research and has become a standard methodology in drug screening and toxicology assays. However, the traditional cell culture methodology-consisting essentially of the immersion of a large population of cells in a homogeneous fluid medium-has become increasingly limiting, both from a fundamental point of view (cells in vivo are surrounded by complex spatiotemporal microenvironments) and from a practical perspective (scaling up the number of fluid handling steps and cell manipulations for high-throughput studies in vitro is prohibitively expensive). Micro fabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, the medium composition, as well as the type of neighboring cells surrounding the microenvironment of the cell. In addition, microtechnology is conceptually well suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. Here we review a variety of applications of microfabrication in cell culture studies, with an emphasis on the biology of various cell types.

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Section: Vg Bone Cell Biology On a Chipmentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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“…Microfabrication and fluidic techniques have been used to control cell adhesion and spreading on the m scale and have proven useful for asking basic questions in cell biology (1,3,8,(15)(16)(17)(18)(19). Although previous studies have used surfaces with spatially resolved chemistry to study cell shape͞function relationships and their effect on protein synthesis (1, 3), studies examining in situ gene expression (mRNA) and protein synthesis as a function of the cell projected area and nuclear shape have not been published.…”

mentioning

confidence: 99%

“…An interpenetrating polymer network of poly(acrylamide) and polyethylene glycol [p(AAm-co-EG)] grafted to silane-derivatized glass was used as a nonadhesive surface chemistry amenable to the thermal cycling of PCR (20). Combining photolithographic and photopolymerization techniques, we created islands of adhesive surface chemistry [an amine-terminated silane that preferentially adsorbs vitronectin (18)] surrounded by the p(AAm-co-EG) interpenetrating polymer network. A range of geometric shapes and sizes were created to impart different mechanical environments within different cells maintained on the same culture surface.…”

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confidence: 99%

Engineering gene expression and protein synthesis by modulation of nuclear shape

Thomas

Collier

Sfeir

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

447

326

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The current understanding of the relationships between cell shape, intracellular forces and signaling, nuclear shape and organization, and gene expression is in its infancy. Here we introduce a method for investigating gene-specific responses in individual cells with controlled nuclear shape and projected area. The shape of the nuclei of primary osteogenic cells were controlled on microfabricated substrata with regiospecific chemistry by confining attachment and spreading of isolated cells on adhesive islands. Gene expression and protein synthesis were altered by changing nuclear shape. Collagen I synthesis correlated directly with cell shape and nuclear shape index (NSI), where intermediate values of nuclear distension (6 < NSI < 8) promoted maximum synthesis. Osteocalcin mRNA, a bone-specific differentiation marker, was observed intracellularly by using reverse transcription in situ PCR at 4 days in cells constrained by the pattern and not detected in unconstrained cells of similar projected area, but different NSI. Our data supports the concept of gene expression and protein synthesis based on optimal distortion of the nucleus, possibly altering transcription factor affinity for DNA, transport to the nucleus, or nuclear matrix organization. The combination of microfabricated surfaces, reverse transcription in situ PCR, and NSI measurement is an excellent system to study how transcription factors, the nuclear matrix, and the cytoskeleton interact to control gene expression and may be useful for studying a wide variety of other cell shape͞gene expression relationships.patterning ͉ cell size ͉ osteocalcin ͉ reverse transcription-PCR ͉ photolithography C ell morphology has a profound effect on a range of cellular events, such as proliferation (1-3), differentiation (4-7), cytoskeletal organization (8), and presumably gene expression. Changes to the cytoskeleton lead to altered stress levels imparted on the nucleus (9-11) and could affect organelle and DNA organization and distribution, ultimately altering cell function. For example, the rate of albumin secretion from hepatocytes can be altered by constraining cell size on patterned culture surfaces (3). In human epidermal kareatinocytes cell shape can modulate between terminal differentiation and proliferation (4). Furthermore, cells can be forced to enter the apoptotic cascade when the area on which the cell is allowed to spread is constrained (1). One proposed mechanism for the transduction of cell shape information into gene expression is through mechanical forces transmitted by means of the direct link of the cytoskeleton to the nucleus (9, 12), and in particular to nuclear matrix proteins (NMPs) such as NMP-1 and NMP-2 (13). These architectural transcription factors, which are components of the nuclear scaffold, induce changes in DNA supercoiling and can interact directly with gene promoter sequences (14). This interaction between the nuclear architecture and regulation of transcription or DNA topography raises the question of whether gross deformation of th...

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“…It was reported that the interaction between a biomaterial and MG-63 cells depends on the topography [49][50][51][52][53], wettability [53,54] and surface energy and charge [55,56] of the biomaterial surface. We assumed that the cell adhesion was suppressed by the surface structure of HAp-coated HNS during the initial stage of the culture.…”

Section: Mg-63 Cell Culture On Cap-coated Hnsmentioning

confidence: 99%

Preparation and biological evaluation of hydroxyapatite-coated nickel-free high-nitrogen stainless steel

Sasaki

Inoue

Katada

et al. 2012

Science and Technology of Advanced Materials

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Calcium phosphate was formed on nickel-free high-nitrogen stainless steel (HNS) by chemical solution deposition. The calcium phosphate deposition was enhanced by glutamic acid covalently immobilized on the surface of HNS with trisuccinimidyl citrate as a linker. X-ray diffraction patterns and Fourier transform infrared spectra showed that the material deposited on glutamic acid-immobilized HNS within 24 h was low-crystallinity calcium-deficient carbonate-containing hydroxyapatite (HAp). The biological activity of the resulting HAp-coated HNS was investigated by using a human osteoblast-like MG-63 cell culture. The HAp-coated HNS stimulated the alkaline-phosphate activity of the MG-63 culture after 7 days. Therefore, HAp-coated HNS is suitable for orthopedic devices and soft tissue adhesion materials.

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The role of vitronectin in the attachment and spatial distribution of bone-derived cells on materials with patterned surface chemistry

Cited by 159 publications

References 35 publications

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Engineering gene expression and protein synthesis by modulation of nuclear shape

Preparation and biological evaluation of hydroxyapatite-coated nickel-free high-nitrogen stainless steel

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