The roles and applications of neural stem cells in spinal cord injury repair

Guo, Wenjuan; Zhang, Xindan; Zhai, Jiliang; Xue, Jiajia

doi:10.3389/fbioe.2022.966866

Cited by 28 publications

(14 citation statements)

References 163 publications

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“…Various cell types have been studied as sources of cell replacement therapy for SCI treatment. Although some issues must be overcome, the use of neuronal stem cells/neuronal precursor cells (NSCs/NPCs), derived from embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), is promising [ 4 , 5 ]. Several studies using animal models of SCI have demonstrated that locomotion is recovered, after SCI, by the transplantation of NSCs/NPCs derived from ESCs and iPSCs [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Transplantation of Chemical Compound-Induced Cells from Human Fibroblasts Improves Locomotor Recovery in a Spinal Cord Injury Rat Model

Kurahashi,

Nishime,

Nishinaka

et al. 2023

IJMS

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The development of regenerative medicine using cell therapy is eagerly awaited for diseases such as spinal cord injury (SCI), for which there has been no radical cure. We previously reported the direct conversion of human fibroblasts into neuronal-like cells using only chemical compounds; however, it is unclear whether chemical compound-induced neuronal-like (CiN) cells are clinically functional. In this study, we partially modified the method of inducing CiN cells (termed immature CiN cells) and examined their therapeutic efficacy, in a rat model of SCI, to investigate whether immature CiN cells are promising for clinical applications. Motor function recovery, after SCI, was assessed using the Basso, Beattie, and Bresnahan (BBB) test, as well as the CatWalk analysis. We found that locomotor recovery, after SCI in the immature CiN cell-transplanted group, was partially improved compared to that in the control group. Consistent with these results, magnetic resonance imaging (MRI) and histopathological analyses revealed that nerve recovery or preservation improved in the immature CiN cell-transplanted group. Furthermore, transcriptome analysis revealed that immature CiN cells highly express hepatocyte growth factor (HGF), which has recently been shown to be a promising therapeutic agent against SCI. Our findings suggest that immature CiN cells may provide an alternative strategy for the regenerative therapy of SCI.

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Section: Introductionmentioning

confidence: 99%

Transplantation of Chemical Compound-Induced Cells from Human Fibroblasts Improves Locomotor Recovery in a Spinal Cord Injury Rat Model

Kurahashi,

Nishime,

Nishinaka

et al. 2023

IJMS

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“…NSCs play important roles in nervous system development and neurogenesis. NSCs can differentiate into neurons and neurogliocytes, maintaining the capability of disintegration, multiplication, and maturity in nerve injury and neurodegenerative disorders 17 . However, NSCs still lack the ability to promote proliferation, migration, and differentiation in adults 18 .…”

Section: Discussionmentioning

confidence: 99%

Photobiomodulation therapy enhances neural differentiation of dental pulp stem cells via ERK1/2 signaling pathway

Zhang,

Li,

Tang

et al. 2023

Photochem & Photobiology

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Photobiomodulation therapy (PBMT) is the application of a low‐level laser device to generate physiological changes and provide therapeutic effects. Till now, the effects of PBMT on the neural differentiation of mesenchymal stem cells have been rarely reported. Herein, the potential effect and mechanism of PBMT on the neural differentiation of dental pulp stem cells (DPSCs) were preliminarily investigated in our research. The optimal dose of 3.75 J/cm2 was first screened for use in the following neural‐inducing studies. Then, DPSCs were cultured in neural induction medium and treated with laser irradiation for 7 days. From the results of morphology and immunofluorescence, we found that irradiation promoted the formation of neural stem cell‐like spheroids derived from DPSCs and enhanced potential neural differentiation. Furthermore, neural differentiation gene expressions of Nestin, microtubule‐associated protein‐2, and neural cell adhesion molecule were increased after PBMT irradiation. The protein expressions of class III β‐tubulin and neurogenic differentiation factor 1 were also improved. Meanwhile, the involvement of extracellular signal‐regulated kinase (ERK1/2) was investigated by western blot. Our study showed that the neural differentiation of DPSCs was promoted by PBMT, and the underlying mechanism in this process was associated with activating the ERK1/2 signaling pathway.

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“…However, neurogenesis is the main obstacle to successful SCI repair. [ 18 ] Modifications should be made to endow MEX with the neural regenerative ability. Laminin is an extracellular matrix protein consisting of α , β , and γ polypeptide chains which regulates many cellular functions of neuronal cells.…”

Section: Introductionmentioning

confidence: 99%

Engineering of M2 Macrophages‐Derived Exosomes via Click Chemistry for Spinal Cord Injury Repair

Zeng

Sun

et al. 2023

Adv Healthcare Materials

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Spinal cord injury (SCI) is one of the most common causes of death and disability. The effective modulation of complicated microenvironment, regeneration of injured spinal cord tissue, and the functional recovery after SCI are still clinical challenges. Recently, macrophages‐derived exosomes have shown great potential for various diseases due to their inflammation‐targeting property. However, further modifications are needed to endow exosomes with the neural regenerative potential for SCI recovery. In the current study, a novel nanoagent (MEXI) is designed for SCI treatment by conjugating bioactive IKVAV peptides to the surface of M2 macrophages‐derived exosomes via an easy and rapid click chemistry method. In vitro, MEXI inhibits the inflammation by reprograming macrophages and promotes neuronal differentiation of neural stem cells. In vivo, engineered exosomes target the injured site of the spinal cord after tail vein injection. Furthermore, histological analysis reveals that MEXI improves motor functional recovery of SCI mice by reducing infiltration of macrophages, downregulating pro‐inflammatory factors, and improving the regeneration of injured nervous tissues. Taken together, this study provides strong evidence for the significance of MEXI in SCI recovery.

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The roles and applications of neural stem cells in spinal cord injury repair

Cited by 28 publications

References 163 publications

Transplantation of Chemical Compound-Induced Cells from Human Fibroblasts Improves Locomotor Recovery in a Spinal Cord Injury Rat Model

Transplantation of Chemical Compound-Induced Cells from Human Fibroblasts Improves Locomotor Recovery in a Spinal Cord Injury Rat Model

Photobiomodulation therapy enhances neural differentiation of dental pulp stem cells via ERK1/2 signaling pathway

Engineering of M2 Macrophages‐Derived Exosomes via Click Chemistry for Spinal Cord Injury Repair

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