The roles played by mitochondrial DNA and nuclear genes in reversion to fertility in S-Type male-sterile maize. Progress report, April 1, 1985--March 31, 1989

Abstract

THE ROLES PLAYED BY MITOCHONDRIAL DNA AND NUCLEAR GENES I N REVERSION TO FERTILITY I N S-TYPE MALE-STERILE MAIZE P e r i o d :C o n t r a c t : P r i n c i p a l I n v e s t i g a t o r : I n s t i t u t i o n :Department: S t a r t i n g Date:. Items: CURRENT CONTRACT A p r i l 1 , 19851 , through March 31, 1990 DE-AC02-81 ER10873 John R. Laughnan U n i v e r s i t y of I l l i n o i s , Urbana, I L P I a n t Biology A p r i l , 1 , 1985 Progress Report 61801 DISCLAIMERThis report was prepared as an accouht of work sponsored by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recornmendation, or favoring by the United States Government or any agency thereof. Over the four-year period of the DOE grant, as before, we have continued collaborations with other laboratories where studies related to ours are underway. We have had a long-standing cooperative arrangement with Chris Leaver at the University of Edinburgh. A joint paper with Leaver and others comparing cytoplasmic revertants to fertility from different cms-S sources has recently been published. nonchromosomal stripe (NCS) mutants. NCS mutations are due to mtDNA alterations. We pollinate these mutants when possible and establish NCS strains. We are collaborating with Kathy Newton at the University of Missouri on the analyses of a number of NCS strains. Two papers resulting from these collaborations have recently been submitted for publication.University of Utah on a comparative study of cms-T and cms-P mtDNAs. already has a cms-T WF9 library and map. We have supplied her with seed of cms-P WF9 with which to begin the studies. This laboratory has identified an inbred line which restores cms-P but not cms-T. This summer we will be making F l s of various isogenic cms-T and cms-P strains with this inbred line and Christiane will be studying the basis for the.differentia1 restoration at the DNA and RNA levels.In the course of the work in our nurseries we occasionally come across