The Russian Human Radiobiological Tissue Repository: A Unique Resource for Studies of Plutonium-Exposed Workers

Loffredo, Christopher A.; Goerlitz, David; Sn, Sokolova; Leondaridis, Leonidas; Zakharova, M. L.; Revina, Valentina; Kirillova, E N

doi:10.1093/rpd/ncw303

Cited by 19 publications

(8 citation statements)

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“…Application of this feature to contaminated samples could facilitate differential localization of a mixed alpha/gamma contaminant (e.g., Pu-238 from Am-241). While the re-use of archival tissues is becoming important in the frame of the 3Rs of ethical animal research (aka, as Replacement, Reduction, Refinement) [44], these functionalities could facilitate and make faster the exploration of sections from archival tissues from animal studies or indeed human studies [45]. …”

Section: Resultsmentioning

confidence: 99%

Actinide bioimaging in tissues: Comparison of emulsion and solid track autoradiography techniques with the iQID camera

et al. 2017

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This work presents a comparison of three autoradiography techniques for imaging biological samples contaminated with actinides: emulsion-based, plastic-based autoradiography and a quantitative digital technique, the iQID camera, based on the numerical analysis of light from a scintillator screen. In radiation toxicology it has been important to develop means of imaging actinide distribution in tissues as these radionuclides may be heterogeneously distributed within and between tissues after internal contamination. Actinide distribution determines which cells are exposed to alpha radiation and is thus potentially critical for assessing absorbed dose. The comparison was carried out by generating autoradiographs of the same biological samples contaminated with actinides with the three autoradiography techniques. These samples were cell preparations or tissue sections collected from animals contaminated with different physico-chemical forms of actinides. The autoradiograph characteristics and the performances of the techniques were evaluated and discussed mainly in terms of acquisition process, activity distribution patterns, spatial resolution and feasibility of activity quantification. The obtained autoradiographs presented similar actinide distribution at low magnification. Out of the three techniques, emulsion autoradiography is the only one to provide a highly-resolved image of the actinide distribution inherently superimposed on the biological sample. Emulsion autoradiography is hence best interpreted at higher magnifications. However, this technique is destructive for the biological sample. Both emulsion- and plastic-based autoradiography record alpha tracks and thus enabled the differentiation between ionized forms of actinides and oxide particles. This feature can help in the evaluation of decorporation therapy efficacy. The most recent technique, the iQID camera, presents several additional features: real-time imaging, separate imaging of alpha particles and gamma rays, and alpha activity quantification. The comparison of these three autoradiography techniques showed that they are complementary and the choice of the technique depends on the purpose of the imaging experiment.

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Section: Resultsmentioning

confidence: 99%

Actinide bioimaging in tissues: Comparison of emulsion and solid track autoradiography techniques with the iQID camera

et al. 2017

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“…Since 2010, biobanking has developed rapidly in Russia, and *30 research biobanks are currently operating nationally, in addition to a few clinical biobanks. [11][12][13][14] Depending on the model and affiliation, they provide services either directly to the institution to which they belong or to a research consortium at a national or international level. Specializations of active Russian research biobanks vary from population-based to dedicated specific clinical areas (e.g., immunology or age-related disorders), as well as those covering multiple clinical domains.…”

Section: Biobank Impact Assessmentmentioning

confidence: 99%

Biobanking Spotlight on Europe, Middle East, and Africa: Presenting the Collective Experience of the ISBER-EMEA Regional Ambassadors

Afifi

Anisimov

Aguilar-Quesada

et al. 2020

Biopreservation and Biobanking

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“…Human material is also archived in SUBI. The Russian Radiobiological Human Tissue Repository (RHTR) was established to collect and store biological samples relevant to the human health effects of chronic, low-dose radiation exposure (Loffredo et al 2017). The RHTR enrolled two cohorts between 1951 and 2017: exposed workers at the Mayak facilities and, as controls, local residents who were never occupationally exposed to ionizing radiation.…”

Section: Southern Urals Biophysical Institute (Subi) Russiamentioning

confidence: 99%

Big data in radiation biology and epidemiology; an overview of the historical and contemporary landscape of data and biomaterial archives

Schofield

Kulka

Tapio

et al. 2019

International Journal of Radiation Biology

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Over the past 60 years a great number of very large datasets have been generated from the experimental exposure of animals to external radiation and internal contamination. This accumulation of 'big data' has been matched by increasingly large epidemiological studies from accidental and occupational radiation exposure, and from plants, humans and other animals affected by environmental contamination. We review the creation, sustainability and reuse of this legacy data, and discuss the importance of Open data and biomaterial archives for contemporary radiobiological sciences, radioecology and epidemiology. We find evidence for the ongoing utility of legacy datasets and biological materials, but that the availability of these resources depends on uncoordinated, often institutional, initiatives to curate and archive them. The importance of open data from contemporary experiments and studies is also very clear, and yet there are few stable platforms for their preservation, sharing, and reuse. We discuss the development of the ERA and STORE data sharing platforms for the scientific community, and their contribution to FAIR sharing of data. The contribution of funding agency and journal policies to the support of data sharing is critical for the maximum utilisation and reproducibility of publicly funded research, but this needs to be matched by training in data management and cultural changes in the attitudes of investigators to ensure the sustainability of the data and biomaterial commons.

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The Russian Human Radiobiological Tissue Repository: A Unique Resource for Studies of Plutonium-Exposed Workers

Cited by 19 publications

References 0 publications

Actinide bioimaging in tissues: Comparison of emulsion and solid track autoradiography techniques with the iQID camera

Actinide bioimaging in tissues: Comparison of emulsion and solid track autoradiography techniques with the iQID camera

Biobanking Spotlight on Europe, Middle East, and Africa: Presenting the Collective Experience of the ISBER-EMEA Regional Ambassadors

Big data in radiation biology and epidemiology; an overview of the historical and contemporary landscape of data and biomaterial archives

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