The same TCR (N)Dβ(N)Jβ junctional region is associated with several different vβ13 subtypes in a multiple sclerosis patient at the onset of the disease

A biased Vα24+ T-cell repertoire leads to circulating NKT-cell defects in a multiple sclerosis patient at the onset of his disease

“…Using the same experimental conditions, we have previously shown in patient MS (A), that very low amounts of cDNA corresponded to 22 distinct Vß13 + (N)Dß(N)Jß sequences infiltrating the CSF. Therefore, restricted repertoire obtained herein was not due to stochastic events of sampling [24]. In contrast to what was observed for V␣24 + chains, sequencing of the V␣5 + allele did not reveal two different profiles between patients and control individuals Fig.…”

“…This persistence of the V␣24-CVVSRENNARLMFG-J␣31 chain was remarkable because we previously showed, in the same patient, a skewing of the Vß repertoire towards expansion of the multiallelic Vß13 family in both the CSF and the peripheral blood. We randomly cloned and sequenced the restricted Vß13 + Jß2.5 + PCR products, which were too diverse to identify a sequence repeated in different samples [24]. The V␣24-CVVSRENNARLMFGDG-J␣31 sequence was used for an advanced blast search.…”

Characterization of the T cell receptor repertoire in the Japanese neuromyelitis optica: T cell activity is up-regulated compared to multiple sclerosis

“…Clonal expansions of selective TCR Vβs including Vβ1 and Vβ13 in NMO suggest that T cells react clonally with autoantigens that induce autoimmune inflammation. Several investigators have reported the association between myelin basic protein (MBP) and TCR Vβ13 [21,22]. Hong et al demonstrated that Vβ13.1 + T cell clones established from MS patients recognized the immunodominant peptide (residues 83-99) of MBP, and those T cells shared an identical CDR3 motif, Vβ13.1-LGRAGLTY.…”

Characterization of CD8+ T cell repertoire in identical twins discordant and concordant for multiple sclerosis

“…[37] a Symbols are used to indicate sequences also reported in other tables: ‚ ϭ Table 2; OE ϭ Table 3; ■ ϭ Table 5; E ϭ Table 6; F ϭ Table 7. b Matching TCRB-CDR3 sequences reported in italics derived from T cells found in active MS lesions [16,34], in CSF [18,35], or in peripheral blood [19] of patients with MS or from MBP-specific [36 -41] and proteolipid protein-specific [40] T lymphocytes. of oligoclonal or monoclonal TCR expansions in diseased compared with healthy co-twins did not reveal significant differences.…”