The scolocidal effect of propolis on protoscoleces and daugther cysts

Abstract

SUMMARYObjective: Scolocidal elements are used in the aim of not scattering the protoscoleces' outside the cyst during the treatment of cyst hydatid in our present-day. It is hoped from the scolocidal materials; besides its effect on protoscoleces, not being toxic, being easy practicable, not causing sclerozan cholanjit and if possible, penetrating in the daughter vesicules. In this research, the effect of propolis which is a bee product on protoscoleces were investigated. Methods: In our work, daughter vesicules and protoscoleces obtain from the liver of a patient during surgery and propolis from the Black Sea Region were used. Viability tests were done by using 0.1% eosine solution. After propolis disolved in dimethylsulfoxide, within the range of 50 mg/mL and 0.010 mg/mL using by 0.9% serum physiological solution 100 µl into each of the tube and in 1.,3.,5.,10.,20.,and 30. minutes the rates of viability of protoscolex (4000 protoscolex/mL) are fixed. The research were subjected to daughter vesicules that is 4-7 mm in diameters. The concentration of propolis at 0.80 and 3.12 mg/mL were investigated viability rates of protoscolex at different incubation time and whether the possibilty of penetration to the daughter vesicules. Results: It was seen that protoscolexs were effected by the Propolis at 0.100 mg/mL concentration in the first minute, at 0.050 mg/mL concentration in the 3. minutes, at 0.025 mg/mL concentration at 10. minutes and at 0.010 mg/mL concentration in 20. minutes. For protoscolex in the vesicules at 0.80 mg/mL concentration in 20. minute, in 3.12 mg/mL concentration in 10. minutes were found to be lethal. Conclusions: As the protoscolex could be killed by Propolis which penetrate to daughter cysts in a very short time as 10 minutes, we thing that it will be beneficial completing advanced studies.

Analysis of Volatile Constituent by Hydrodistillation and Solid‐Phase Microextraction Techniques and Antimicrobial and Scolicidal Activities of Essential Oil and Soxhlet Extracts of Ulva rigida grown in Turkey

“…[42] Özcelik et al (2015) also determined that propolis can act on daughter vesicles within 10 min. [43] In addition, Karaman et al (2022) reported that the Cystoseria barbata, a brown algae, can be used as a scolicidal agent, while Yücel (2022) reported the same for Cystoseira crinita, which is also a brown algae. [22,23] It was observed that the scolicidal effect of organic solvent extracts of U. rigida prepared in chloroform, dichloromethane, hexane and methanol increased over time.…”

“…(2007) reported that Allium sativum was effective on protoscoleces at 50 mg/mL concentration of at the 15th min. [42] Özcelik et al (2015) also determined that propolis can act on daughter vesicles within 10 min. [43] In addition, Karaman et al (2022) reported that the Cystoseria barbata, a brown algae, can be used as a scolicidal agent, while Yücel (2022) reported the same for Cystoseira crinita, which is also a brown algae.…”

“…(2007) reported that Allium sativum was effective on protoscoleces at 50 mg/mL concentration of at the 15th min [42] . Özcelik et al (2015) also determined that propolis can act on daughter vesicles within 10 min [43] . In addition, Karaman et al.…”

Comparison of Volatile Chemical Components of Cystoseira crinita Duby by HD and SPME Methods and Antimicrobial and Scolicidal Activities Activities of Essential Oil and Extracts

“…Ozcelik et al [53] reported that Allium sativum was effective on protoscolices. Again, Ozcelik et al [54] found that propolis also affected daughter vesicles within 10 minutes. In the study, the M extract of C. crinita showed no live parasites at the 7th hour.…”

Comparison of volatile chemical components of Cystoseira crinita Duby by hydrodistillation(HD) and solid-phase microextraction (SPME) methods and antimicrobialand scolicidal activities of essential oil and extracts

“…Ozcelik et al [ 53 ] reported that Allium sativum was effective on protoscolices. Again, Ozcelik et al [ 54 ] found that propolis also affected daughter vesicles within 10 min. In the study, the M extract of C. crinita showed no live parasites at the 7th h. Additionally, the DCM extract showed no parasites at the 9th h. No viable protoscolices were observed at the 11th h for the Ch and H extracts.…”