The SDF-1/CXCR4 axis promotes recovery after spinal cord injury by mediating bone marrow-derived from mesenchymal stem cells

Wang, Guodong; Liu, Yi‐Xun; Wang, Xiao; Zhang, Yongle; Zhang, Yadong; Xue, Feng

doi:10.18632/oncotarget.14619

Cited by 25 publications

(12 citation statements)

References 42 publications

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“…Therefore, we conclude that, although bone growth after administration of SDF-1 results in a slower progress of bone regeneration, the bone seems to be more physiological than after BMP-7 utilization. This might be due to the fact that SDF-1 promotes bone regeneration via recruitment of endogenous bone marrow-derived stem cells [43,49,50] and cell migration [51]. By loading PLA and collagen with SDF-1, the required cells for bone regeneration are homed.…”

Section: Histological Analysesmentioning

confidence: 99%

Biofabrication of SDF-1 Functionalized 3D-Printed Cell-Free Scaffolds for Bone Tissue Regeneration

Lauer

Wolf

Mehler

et al. 2020

IJMS

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Large segmental bone defects occurring after trauma, bone tumors, infections or revision surgeries are a challenge for surgeons. The aim of our study was to develop a new biomaterial utilizing simple and cheap 3D-printing techniques. A porous polylactide (PLA) cylinder was printed and functionalized with stromal-derived factor 1 (SDF-1) or bone morphogenetic protein 7 (BMP-7) immobilized in collagen type I. Biomechanical testing proved biomechanical stability and the scaffolds were implanted into a 6 mm critical size defect in rat femur. Bone growth was observed via x-ray and after 8 weeks, bone regeneration was analyzed with µCT and histological staining methods. Development of non-unions was detected in the control group with no implant. Implantation of PLA cylinder alone resulted in a slight but not significant osteoconductive effect, which was more pronounced in the group where the PLA cylinder was loaded with collagen type I. Addition of SDF-1 resulted in an osteoinductive effect, with stronger new bone formation. BMP-7 treatment showed the most distinct effect on bone regeneration. However, histological analyses revealed that newly formed bone in the BMP-7 group displayed a holey structure. Our results confirm the osteoinductive character of this 3D-biofabricated cell-free new biomaterial and raise new options for its application in bone tissue regeneration.

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Section: Histological Analysesmentioning

confidence: 99%

Biofabrication of SDF-1 Functionalized 3D-Printed Cell-Free Scaffolds for Bone Tissue Regeneration

Lauer

Wolf

Mehler

et al. 2020

IJMS

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“…The endogenous expression of CXCL12 is increased in response to various injuries and recruits mesenchymal stem cells to the damaged sites enabling tissue repair [37][38][39][40]. With the increased understanding of stem cell biology, recent studies have focused on the potential therapeutic capacities of this chemokine in a variety of conditions using experimental models of locally induced inflammation, nerve damage, or traumatic wounds [16,41,42]. We previously described a murine Asherman syndrome model where CXCL12 augmentation after uterine injury markedly reduced the formation of endometrial fibrosis, suggesting a role for CXCL12 in preventing progressive uterine fibrosis and adhesions [13].…”

Section: Discussionmentioning

confidence: 99%

Bone marrow-derived cells or C-X-C motif chemokine 12 (CXCL12) treatment improve thin endometrium in a mouse model†

Mamillapalli

Şahin

et al. 2018

Biology of Reproduction

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Successful implantation and pregnancy is dependent on sufficient endometrial growth during each reproductive cycle. Here we report the therapeutic effect of either bone marrow-derived cells or the stem cell chemo-attractant CXCL12 on endometrial receptivity in a murine ethanol induced thin endometrium model. Endometrial epithelial area was significantly increased in mice treated with BMDCs, CXCL12 or by co-treatment with both compared with PBS treated controls. Ki-67 and CD31 immunoreactivity was significantly higher in mice treated with either BMDCs, CXCL12 or both. The mRNA expression levels of endometrial receptivity markers leukemia inhibitory factor, interleukin-1β, and integrin beta-3 were increased in mice treated with either BMDCs, CXCL12 or both. The mRNA levels of matrix metalloproteinase-2 and -9 were significantly decreased by BMDCs but not by CXCL12. Pregnancy rates and litter size were increased after either treatment. Both BMDCs and CXCL12 displayed a comparable efficacy on endometrial regeneration in mice with thin endometrium. Our findings indicate the potential therapeutic effects of BMDCs and CXCL12 on infertility related to thin endometrium.

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“…In the present work, we used the microsyringe to direct inject BMSCs into the SCI lesion sites, which is also minimally invasive and favored for cellular delivery into the cerebrospinal fluid. BMSCs have been observed to migrate and accumulate in the damaged areas of the nerve tissue and promote repair in previous studies [ 21 , 22 ]. In our previous studies, we transplanted BMSCs to the injury site in the spinal cord of rats and monkeys.…”

Section: Discussionmentioning

confidence: 99%

Magnetic resonance imaging tracking and assessing repair function of the bone marrow mesenchymal stem cells transplantation in a rat model of spinal cord injury

et al. 2017

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The transplantation of bone marrow mesenchymal stem cells (BMSCs) to repair spinal cord injury (SCI) has become a promising therapy. However, there is still a lack of visual evidence directly implicating the transplanted cells as the source of the improvement of spinal cord function. In this study, BMSCs were labeled with NF-200 promoter and lipase-activated gadolinium-containing nanoparticles (Gd-DTPA-FA). Double labeled BMSCs were implanted into spinal cord transaction injury in rat models in situ, the function recovery was evaluated on 1st, 7th, 14th, 28 th days by MRI, Diffusion Tensor Imaing, CT imaging and post-processing, and histological observations. BBB scores were used for assessing function recovery. After transplantation of BMSCs, the hypersignal emerged in spinal cord in T1WI starting at day 7 that was focused at the injection site, which then increased and extended until day 14. Subsequently, the increased signal intensity area rapidly spread from the injection site to entire injured segment lasting four weeks. The diffusion tensor tractography and histological analysis both showed the nerve fibre from dividing to connecting partly. Immunofluorescence showed higher expression of NF-200 in Repaired group than Injury group. Electron microscopy showed detachment and loose of myelin lamellar getting better in Repaired group compared with the Injury group. BBB scores in Repaired group were significantly higher than those of injury animals. Our study suggests that the migration and distribution of Gd-DTPA-FA labeled BMSCs can be tracked using MRI. Transplantation of BMSCs represents a promising potential strategy for the repair of SCI.

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The SDF-1/CXCR4 axis promotes recovery after spinal cord injury by mediating bone marrow-derived from mesenchymal stem cells

Cited by 25 publications

References 42 publications

Biofabrication of SDF-1 Functionalized 3D-Printed Cell-Free Scaffolds for Bone Tissue Regeneration

Biofabrication of SDF-1 Functionalized 3D-Printed Cell-Free Scaffolds for Bone Tissue Regeneration

Bone marrow-derived cells or C-X-C motif chemokine 12 (CXCL12) treatment improve thin endometrium in a mouse model†

Magnetic resonance imaging tracking and assessing repair function of the bone marrow mesenchymal stem cells transplantation in a rat model of spinal cord injury

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