The search for a unique Raman signature of amyloid-beta plaques in human brain tissue from Alzheimer's disease patients

Lochocki, Benjamin; Morrema, Tjado H. J.; Ariese, Freek; Hoozemans, Jeroen J. M.; Boer, Johannes F. de

doi:10.1039/c9an02087j

Cited by 36 publications

(37 citation statements)

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“…The bright, yellow/orange and uniformly distributed spots, which can be seen in all auto-fluorescence tissue samples (some spots are indicated by blue arrows in Figs. 2a and 3) are lipofuscin granular deposits 23,26 . Lipofuscin, an age-related metabolic waste product, is highly unlikely an indicator or hallmark of AD since it is also found in control cases with a similar distribution 23,27 (see also controls in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Greenish dense cores (#1a and #1b) and greenish patches (#2) are clearly visible. The uniformly distributed yellow spots (blue arrows, just one is identified in each image) are lipofuscin deposits 23 . b Fluorescence images of the same tissue sections and areas stained with thioflavin-S.…”

Section: Resultsmentioning

confidence: 99%

“…Its apparent lower intensity can be explained as a consequence of the background removal algorithm. The raw spectral data of lipofuscin areas contain the highest fluorescence background and relatively little Raman scattering on top of it 23 . The background removal algorithm subtracts most of the underlying fluorescence, resulting in a low-intensity Raman spectrum for the lipofuscin spots.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, we investigated whether Raman spectroscopy (λ exc = 785 nm) could be used for the detection of Aβ plaques in fixed human brain tissue. Despite the potential of vibrational spectroscopic techniques for a label-free and non-invasive characterization of biomolecular composition, a unique Aβ-associated spectrum could not be determined 23 . In the present study, we report on data obtained from post-mortem, snapfrozen human AD tissue.…”

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confidence: 99%

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Multimodal, label-free fluorescence and Raman imaging of amyloid deposits in snap-frozen Alzheimer’s disease human brain tissue

et al. 2021

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Alzheimer’s disease (AD) neuropathology is characterized by hyperphosphorylated tau containing neurofibrillary tangles and amyloid-beta (Aβ) plaques. Normally these hallmarks are studied by (immuno-) histological techniques requiring chemical pretreatment and indirect labelling. Label-free imaging enables one to visualize normal tissue and pathology in its native form. Therefore, these techniques could contribute to a better understanding of the disease. Here, we present a comprehensive study of high-resolution fluorescence imaging (before and after staining) and spectroscopic modalities (Raman mapping under pre-resonance conditions and stimulated Raman scattering (SRS)) of amyloid deposits in snap-frozen AD human brain tissue. We performed fluorescence and spectroscopic imaging and subsequent thioflavin-S staining of the same tissue slices to provide direct confirmation of plaque location and correlation of spectroscopic biomarkers with plaque morphology; differences were observed between cored and fibrillar plaques. The SRS results showed a protein peak shift towards the β-sheet structure in cored amyloid deposits. In the Raman maps recorded with 532 nm excitation we identified the presence of carotenoids as a unique marker to differentiate between a cored amyloid plaque area versus a non-plaque area without prior knowledge of their location. The observed presence of carotenoids suggests a distinct neuroinflammatory response to misfolded protein accumulations.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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Multimodal, label-free fluorescence and Raman imaging of amyloid deposits in snap-frozen Alzheimer’s disease human brain tissue

et al. 2021

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“…On the other hand, the group of Sudan dyes [7][8][9] is the group of azo dyes, widely used in histological investigations. Moreover, being fat-soluble, they are used to color vax, oils, petrol derivatives, solvents, and polishes in bright colors-red, orange, and yellow [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

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2020

Lett Appl NanoBioSci

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In this work, a general theoretical description of the electrochemical determination of Sudan dyes by cathodic and anodic route has been made. Two mathematical models have been developed for each case, being, afterward, analyzed by means of linear stability theory and bifurcation analysis. It has been shown that in both cases, the systems are efficient from the electroanalytical point of view. The possibility for the oscillatory and monotonic instability has also been verified.

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Advances in tissues and cells characterization by Raman micro‐spectroscopy, atomic force microscopy, and tip‐enhanced Raman spectroscopy

Batista

Foiani

Champeau

et al. 2022

J Raman Spectroscopy

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The comprehension of some diseases at the cellular and tissue scale is fundamental for the development of effective treatments for such conditions, and in this regard, Raman micro‐spectoscopy, atomic force microscopy (AFM), and tip‐enhanced Raman scattering (TERS) are techniques that have featured prominently in the characterization of biological materials like cells and tissues, since they allow the acquisition of detailed physical, chemical, biophysical, and physiological properties of the surface of these kind of samples such as elasticity, viscoelasticity, wettability, topography, molecular composition, and others. Due to this, this literature review has as its primary scope the presentation of some of the recent advances and new analysis methodologies using Raman, AFM, and TERS, pointing out what they can offer toward the investigation of the behavior of cells and biological tissues. Limits of usage, drawbacks, and future perspectives are presented and discussed.

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The search for a unique Raman signature of amyloid-beta plaques in human brain tissue from Alzheimer's disease patients

Abstract: Raman spectroscopy was used to examine unstained, formalin fixed Alzheimer's disease human brain tissue to potentially identify a unique spectral signature of amyloid-beta plaques.

Cited by 36 publications

References 67 publications

Multimodal, label-free fluorescence and Raman imaging of amyloid deposits in snap-frozen Alzheimer’s disease human brain tissue

Multimodal, label-free fluorescence and Raman imaging of amyloid deposits in snap-frozen Alzheimer’s disease human brain tissue

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Advances in tissues and cells characterization by Raman micro‐spectroscopy, atomic force microscopy, and tip‐enhanced Raman spectroscopy

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