The search for plants with anticancer activity: Pitfalls at the early stages

Taylor, Peter; Colman, Laura; Bajoon, J.

doi:10.1016/j.jep.2014.10.034

Cited by 13 publications

(14 citation statements)

References 45 publications

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“…Here, the cytotoxicity of one aqueous‐ethanolic extract from the marine angiosperm T. testudinum which grows in the Caribbean Sea and it is rich in this kind of structures was assayed in a panel of tumour and non‐tumour cells (Table ). Cell viability was determined using two individual methods based on different criteria, as it has been recommended . This primary screening showed that under our experimental conditions, the exposure to different concentrations of T. testudinum extract up to 1000 μg/ml did not induce significant toxicity effects in normal cells, meanwhile above 100 μg/ml, the extract induced loss of cell viability in the tested tumour cell lines.…”

Section: Resultsmentioning

confidence: 94%

“…The IC 50 , derived from the MTT and other such assays, is an important first step in the detection of inhibitory activity against tumour cells . Based on the IC 50 values, the extract did not seems to be highly cytotoxic.…”

Section: Resultsmentioning

confidence: 99%

“…DNA damage is a primary event which induces cell death mainly by apoptosis, activating the p53 pathway . The majority of the cytostatic drugs in clinical use at present are DNA‐targeting agents which induce apoptotic cell death in vitro ; however, their main drawback is the lack of selectivity for tumour cells, causing irreversible DNA damage and cell death also in normal healthy tissues …”

Section: Resultsmentioning

confidence: 99%

“…Cell viability tests complemented with other criteria for cell damage or toxicity and a careful interpretation of the results allow the identification of novel antitumour compounds in high‐throughput screenings. [ This kind of approach has been used to identify new cytotoxic and antitumour products from natural sources including marine plants and seaweeds …”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Assessment of the cytotoxic potential of an aqueous-ethanolic extract from Thalassia testudinum angiosperm marine grown in the Caribbean Sea

Rodeiro

Hernández

Herrera

et al. 2018

Journal of Pharmacy and Pharmacology

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show abstract

Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Assessment of the cytotoxic potential of an aqueous-ethanolic extract from Thalassia testudinum angiosperm marine grown in the Caribbean Sea

Rodeiro

Hernández

Herrera

et al. 2018

Journal of Pharmacy and Pharmacology

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show abstract

“…In fact, some authors reported the problems of misdiagnosis by traditional healers for some diseases that were not common in African areas. These diseases often called emerging diseases in Africa included cancers and metabolic diseases like hypertension (Alonso-Castro et al, 2011; Taylor et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Ethnobotanical Survey of Medicinal Plants Used in the Management of Hypertension in the Maritime Region of Togo

Gbekley¹,

Karou²,

Katawa³

et al. 2017

AJTCAM

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Background: Hypertension is one of the most common chronic diseases in modern societies and there is evidence that its incidence and severity are increasing. This survey was conducted to investigate the plants used by the Togolese traditional healers to treat the disease. Method: From January to June 2016, an ethnobotanical survey was conducted using a semi-structured questionnaire with traditional healers (TH) in the southern region of Togo. The importance of the plants was assessed by the calculation of the use value (UV). Results: In Total, 128 TH male and female were interviewed and 116 plants species belonging to 46 families were identified as treating hypertension. The most represented families were: Fabaceae with 16 species followed by Euphorbiaceae and Rutaceae contributing with 8 and 6 species respectively. Based on the calculated use values the most important species were Byrsocarpus coccineus Schum. The leaves and the roots were the parts of plant predominantly used to prepare the recipes, mainly decoctions administrated by oral route. Clinical manifestations such as dizzy spells, swarming, loss of consciousness, severe headache, severe anxiety and shortness of breath, nosebleed, and fear of heights were used by TH to diagnose the disease. Conclusion: This study showed initial evidence of the use of plant materials by Togolese TH to treat hypertension. These results constitute a database for pharmacological screenings with the aim of developing new therapies.

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High‐content cell death imaging using quantum dot‐based TIRF microscopy for the determination of anticancer activity against breast cancer stem cell

Shim

Song

2016

Journal of Biophotonics

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We report a two color monitoring of drug-induced cell deaths using total internal reflection fluorescence (TIRF) as a novel method to determine anticancer activity. Instead of cancer cells, breast cancer stem cells (CSCs) were directly tested in the present assay to determine the effective concentration (EC ) values of camptothecin and cisplatin. Phosphatidylserine and HMGB1 protein were concurrently detected to observe apoptotic and necrotic cell death induced by anticancer drugs using quantum dot (Qdot)-antibody conjugates. Only 50-to-100 breast CSCs were consumed at each cell chamber due to the high sensitivity of Qdot-based TIRF. The high sensitivity of Qdot-based TIRF, that enables the consumption of a small number of cells, is advantageous for cost-effective large-scale drug screening. In addition, unlike MTT assay, this approach can provide a more uniform range of EC values because the average values of single breast CSCs fluorescence intensities are observed to acquire EC values as a function of dose. This research successfully demonstrated the possibility that Qdot-based TIRF can be widely used as an improved alternative to MTT assay for the determination of anticancer drug efficacies.

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The search for plants with anticancer activity: Pitfalls at the early stages

Cited by 13 publications

References 45 publications

Assessment of the cytotoxic potential of an aqueous-ethanolic extract from Thalassia testudinum angiosperm marine grown in the Caribbean Sea

Assessment of the cytotoxic potential of an aqueous-ethanolic extract from Thalassia testudinum angiosperm marine grown in the Caribbean Sea

Ethnobotanical Survey of Medicinal Plants Used in the Management of Hypertension in the Maritime Region of Togo

High‐content cell death imaging using quantum dot‐based TIRF microscopy for the determination of anticancer activity against breast cancer stem cell

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