The search for the location of the sunflower resistance gene to race G of broomrape on the linkage map in the lines of breeding of VNIIMK

Abstract

The parasitic plant Orobanche cumana Wallr. is one of the main biotic limiting factors for obtaining high yields of sunflower. Since plant resistance to different broomrape races in different genotypes can be controlled by different genes, to ensure the evaluation of breeding material using DNA markers (MAS), it is necessary to localize the resistance gene for each of the sources. The aim of the research is to find the location of the Or7 gene, which controls the resistance to one of the most virulent broomrape races, on the sunflower linkage map and to determine the DNA markers cosegregating with it. For research we used the lines of breeding of V.S. Pustovoit of All-Russian Research Institute of Oil Crops (VNIIMK) resistant and susceptible to race G of broomrape. We used SSR-primers ORS 683, ORS 1040, ORS 1112, and ORS 202 for the PCR analysis. We excluded the location of the gene in the upper part of LG3 in the immediate proximity to the microsatellite loci ORS 683, ORS 1040, ORS 1112. Based on the studied literary sources and the reference genome of the sunflower HanXRQr2.0-SUNRISE, we compiled a partial physical map of LG3 and determined the area for further search for the localization of the Or7 gene and DNA markers cosegregating with it.