The sensitivity and the specifity of rapid antigen test in streptococcal upper respiratory tract infections

Gürol, Yeşim; Akan, Hülya; İzbırak, Güldal; Tekkanat, Z.T.; Gunduz, Tehlile Silem; Hayran, Osman; Yılmaz, Gülden

doi:10.1016/j.ijporl.2010.02.020

Cited by 25 publications

(25 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Where the multiple sensitivity and specificity pairs were estimated from different samples of patients, each pair is treated in our analysis as if it came from a separate study. 6,[17][18][19][20] Where a different RADT was tested in the same sample of patients, we included each sensitivity and specificity pair in the subgroup analysis for the respective RADT. [21][22][23][24][25] Where multiple sensitivity and specificity pairs were estimated from the same patients in a study, only 1 pair of sensitivity and specificity was included in our analysis.…”

Section: Figurementioning

confidence: 99%

Rapid Diagnostic Tests for Group A Streptococcal Pharyngitis: A Meta-analysis

et al. 2014

View full text Add to dashboard Cite

BACKGROUND AND OBJECTIVE: Effective management of group A streptococcal (GAS) pharyngitis is hindered by impracticality of the gold standard diagnostic test: throat culture. Rapid antigen diagnostic tests (RADTs) are a promising alternative, although concerns about their sensitivity and specificity, and variation between test methodologies, have limited their clinical use. The objective of this study was to perform a systematic review with meta-analysis of the diagnostic accuracy of RADTs for GAS pharyngitis. METHODS:Medline and Embase from 1996 to 2013 were used as data sources. Of 159 identified studies, 48 studies of diagnostic accuracy of GAS RADTs using throat culture on blood agar as a reference standard were selected. Bivariate random-effects regression was used to estimate sensitivity and specificity with 95% confidence intervals (CIs). Additional meta-analyses were performed for pediatric data. RESULTS:A total of 60 pairs of sensitivity and specificity from 48 studies were included. Overall summary estimates for sensitivity and specificity of RADTs were 0.86 (95% CI 0.83 to 0.88) and 0.96 (95% CI 0.94 to 0.97), respectively, and estimates for pediatric data were similar. Molecular-based RADTs had the best diagnostic accuracy. Considerable variability exists in methodology between studies. There were insufficient studies to allow meta-regression/subgroup analysis within each test type.CONCLUSIONS: RADTs can be used for accurate diagnosis of GAS pharyngitis to streamline management of sore throat in primary care. RADTs may not require culture backup for negative tests in most low-incidence rheumatic fever settings. Newer molecular tests have the highest sensitivity, but are not true point-of-care tests. ABBREVIATIONS CI-confidence interval ELISA-enzyme-linked immunosorbent assay FISH-fluorescence in situ hybridization GAS-group A b-hemolytic streptococcus OIA-optical immunoassay PCR-polymerase chain reaction QUADAS-Quality Assessment of Diagnostic Accuracy Studies RADT-rapid antigen diagnostic test ROC-receiver operating characteristics S-ROC-summary receiver operating characteristics Dr. Lean collected data for the study and drafted the initial manuscript; Dr Arnup carried out the statistical analyses and reviewed and revised the manuscript; Drs Danchin and Steer conceptualized the study, supervised data collection, and critically reviewed and revised the manuscript; and all authors approved the final manuscript as submitted.www.pediatrics.org/cgi

show abstract

Section: Figurementioning

confidence: 99%

Rapid Diagnostic Tests for Group A Streptococcal Pharyngitis: A Meta-analysis

et al. 2014

View full text Add to dashboard Cite

show abstract

“…RADT is a rapid diagnostic test that assist in the diagnosis of bacterial pharyngitis caused by Group A Streptococci and gives a visual, qualitative detection of Group A streptococcal antigen directly from throat swabs. 7,8 RADT helps patients both immediately and potentially in the long-term by rapid confirmation of Streptococcal cause of their sore throat. Prompt Antibiotic treatment in confirmed cases provides a quicker reduction of symptoms, shortens the duration of illness, and quickly and efficiently eliminates the possibility of spread of infection to others.…”

Section: Introductionmentioning

confidence: 99%

Utility of Rapid Antigen Detection Test for Diagnosis of Streptococcal a Infection Directly From the Throat Swab of Suspected Patients of Pharyngitis

Gupta¹,

Kaore²,

Qureshi³

et al. 2018

jemds

View full text Add to dashboard Cite

BACKGROUND Beta-haemolytic Group A Streptococcus (GAS), i.e. Streptococcus pyogenes is responsible for a wide range of both invasive and non-invasive infections. Early diagnosis and treatment of Group A streptococcal pharyngitis has been shown to reduce the severity of symptoms and further complications such as rheumatic fever and glomerulonephritis. Infection of GAS may spread through direct contact with mucus or sores on the skin with over 10 million mild infections and > 500,000 deaths per year. With 20% to 40% bacterial infections, which are indistinguishable from viral infections can be effectively treated by antibiotics. The 48 to 72 hours delay in culture conformation is problematic in low-resource settings, where repeated follow-up visits are not feasible for appropriate treatment. Rapid Antigen Detection Tests (RADTs) assist in the diagnosis of bacterial pharyngitis caused by Group A Streptococci and gives a visual qualitative detection of Group A streptococcal antigen directly from throat swabs. These are potentially more feasible alternatives, because of their quick turnaround time. MATERIALS AND METHODSThe cross-sectional study was carried out in the Department of Microbiology in college associated with tertiary care hospital, in central India Bhopal during a time period of 2 months from August to September 2016 after obtaining permission from the Institutional Ethics Committee. A total of 38 non-repetitive patients of either gender and of any age clinically suspected to be of pharyngitis attending OPD of Department of ENT in a tertiary care hospital were enrolled after informed consent. Throat swabs collected were transported and processed immediately for the culture and RADT for Streptococcal A. All data was maintained in Microsoft Office Excel and statistical analysis was carried out using Excel with appropriate statistical tools like tests of proportion and significance. RESULTSOut of 38 samples collected, 25 were from male patients and 13 were from females. 35 out of 38 (91.10%) patients were from active age group. All the samples tested were negative for RADT. A total of 16/38 (42.10%) samples grew Beta-haemolytic streptococci, but were negative when again tested for Streptococcal A antigen using RADT. CONCLUSIONThe sensitivity and specificity of Rapid Antigen detection test by immuno-chromatographic tests have been claimed to be over 90%, but could not be established in our settings. A large scale study with strict inclusion criteria is warranted to establish the utility of RADT.

show abstract

“…Apresenta especificidade em torno de 95% quando comparado à cultura de orofaringe (Santos et al, 2005;Camurdan et al, 2008;Cardoso et al, 2009;Lasseter et al, 2009;Tanz et al, 2009;Gurol et al, 2010;Rogo et al, 2010;Ruiz-Aragón et al, 2010;Cohen et al, 2012;Cardoso et al, 2013), e por essa razão, preconiza-se que nos casos em que a PRE for positiva não se realize cultura de orofaringe concomitante (Armengol et al, 2004;Edmonson;Farwell, 2005;Weber, 2014). Resultados de PRE falso positivo são raros, porém podem ocorrer quando há a presença de outros estreptocococos na orofaringe, como Streptococcus milleri, alfa-hemolíticos, que expressam em sua membrana o antígeno do carboidrato do grupo A.…”

Section: A Faringotonsiliteunclassified

“…Dessa forma, recomenda-se que frente a uma prova rápida negativa proceda-se a coleta de cultura de orofaringe (Webb, 1998;Woods, 1999;Linder et al, 2005;Fontes et al, 2007;Morais et al, 2009 (Santos et al, 2005;Camurdan et al, 2008;Lasseter et al, 2009;Tanz et al, 2009;Gurol et al, 2010;Rogo et al, 2010;Ruiz-Aragón et al, 2010;Cohen et al, 2012) O uso criterioso de antibiótico desempenha um papel importante na prevenção da resistência bacteriana aos antimicrobianos, na redução dos custos de cuidados na saúde, e na melhora do atendimento individual ao paciente (Deuster et al, 2010).…”

Section: A Faringotonsiliteunclassified

“…Entretanto, com o surgimento da PRE e, em função de ser um exame com resultado precoce, pode-se utilizar tal método diagnóstico como triagem para a definição da conduta, usar antibiótico ou não, num primeiro momento. Vários autores recomendam que frente a um resultado negativo da PRE seja realizada a cultura como exame comprobatório (Webb, 1998;Woods, 1999; American Academy of Pediatrics, 2000; Atlas et al, 2005;2009;Morais et al, 2009;Mateo et al, 2010;Giraldez-Garcia et al, 2011;Regoli et al, 2011 (Needham et al, 1998;Webb, 1998;McIsaac et al, 2000;Giesseker et al, 2002;Edmonson;Farwell, 2005;Santos et al, 2005;Buchbinder et al, 2007;Fontes et al, 2007;Al-Najar, 2008;Cardoso et al, 2009;Lasseter et al, 2009;Tanz et al, 2009;Gurol et al, 2010;Rogo et al, 2010;Ruiz-Aragón et al, 2010;Sarikaya et al, 2010;Regueras De Lorenzo et al, 2012;Cohen et al, 2012;Lean et al, 2014).…”

Section: D I I S S C C U U S S S S ã ã O Ounclassified

See 1 more Smart Citation

Impacto do uso de técnicas microbiológicas para o estreptococo beta hemolítico do grupo A no diagnóstico e tratamento das faringotonsilites

Cardoso¹

View full text Add to dashboard Cite

Dedico essa obra a minha família, meu bem mais precioso Ao meu marido, Rodrigo, meu primeiro e grande amor! Meu companheiro e incentivador de todas as horas.Aos meus filhos, Beatriz e Tiago, que me tornaram uma pessoa melhor e me apresentaram ao sentimento mais pleno que existe, o AMOR incondicional.Para eles e por eles busco sempre ser melhor... Aos meus pais, Ana Maria e Flávio, sempre incentivadores do meu trabalho! Pessoas maravilhosas que investiram na formação dos meus valores morais e éticos, além de sempre terem valorizado a minha educação e terem investido na minha formação. Sempre com muito amor! Graças a esses valores concluo essa etapa tão importante de minha vida... Aos meus irmãos, Andrea e Flávio, por sua amizade e companheirismo, e por sempre me admirarem e confiarem a mim os cuidados aos seus grandes tesouros, Rafael, Isabella e Gabriella. A A G G R R A A D D E E C C I I M M E E N N T T O O S S

show abstract

The sensitivity and the specifity of rapid antigen test in streptococcal upper respiratory tract infections

Cited by 25 publications

References 13 publications

Rapid Diagnostic Tests for Group A Streptococcal Pharyngitis: A Meta-analysis

Rapid Diagnostic Tests for Group A Streptococcal Pharyngitis: A Meta-analysis

Utility of Rapid Antigen Detection Test for Diagnosis of Streptococcal a Infection Directly From the Throat Swab of Suspected Patients of Pharyngitis

Impacto do uso de técnicas microbiológicas para o estreptococo beta hemolítico do grupo A no diagnóstico e tratamento das faringotonsilites

Contact Info

Product

Resources

About