The Sertoli cell occluding junctions and gap junctions in mature and developing mammalian testis

Gilula, Norton B.; Fawcett, Don W.; Aoki, Agustı́n

doi:10.1016/0012-1606(76)90074-9

Cited by 300 publications

(152 citation statements)

References 30 publications

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“…No obvious junctional strands and their networks could be found in the myelinated nerve. The linear arrangement of junctional particles observed in the present study was reminiscent of the intermediate stage of a developing tight junction of embryonic epithelial and mesothelial cells in various species MONTESANO, 1975;TICE et al, 1977;NAGANO and SUZUKI, 1976a;SUZUKI and NAGANO, 1979), and also of the tight junctions in some vascular endothelia (YEE and REVEL, 1975;SIMIONESCU et al, 1975SIMIONESCU et al, , 1976 Sertoli cells (GILULA et al, 1976;NAGANO and SUZUKI, 1976b) and the proximal convoluted tubule of the kidney (CLAUDE and GOODENOUGH, 1973) of adult animals. Thus, the mesaxonal tight junction is generally leaky or loose in structure.…”

Section: Discussionsupporting

confidence: 54%

Mesaxonal membrane junction of myelinated nerve in the central and peripheral nervous system.

Inokuchi

Higashi

1980

Arch. Histol. Jap., Arch. Histol. Jpn

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Section: Discussionsupporting

confidence: 54%

Mesaxonal membrane junction of myelinated nerve in the central and peripheral nervous system.

Inokuchi

Higashi

1980

Arch. Histol. Jap., Arch. Histol. Jpn

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“…A particle-free region between two rows of particles in the double strands in this study seems to be much wider than that reported by NAGANO et al (1977). In contrast to the usual grooves in previous investigations (WADE and KARNOVSKY, 1974;SIMIONESCU et al, 1975SIMIONESCU et al, , 1976GILULA et al, 1976;SUZUKI, 1976a, 1976b;NAGANO et al, 1977), the structures reported in the present study deserve attention in two points: First, the width of type I or type II grooves in this study appears to be wider, and second, type II groove shown in Figure 8 is clearly distinguished into two "sub-grooves" and in some areas a strand of particles appears to be located between them.…”

Section: Discussioncontrasting

confidence: 75%

“…On the P-face, not only grooves but also double linear arrangements of intramembraneous particles have been shown by CLAUDE and GOODENOUGH (1973) and STAEHELIN (1973), and by NAGANO et al (1977). On the other hand, the strand preferentially located on the E-face has been described by several investigators (SIMIONESCU et al, 1975(SIMIONESCU et al, , 1976GILULA et al, 1976;SUZUKI, 1976a, 1976b;NAGANO et al, 1977). However, there seem to be no reports concerning the particular area which displays simultaneously both double strands of intramembraneous particles on the P-face and single strands at the bottom of a wide groove or between two narrow grooves on the complementary E-face.…”

mentioning

confidence: 84%

An Unusual Architecture of Occluding Junctions between Surface Cells in Teleost Ovarian Follicles (<i>Plecoglossus altivelis</i>)

Toshimori

Ōura

1979

Arch. Histol. Jap., Arch. Histol. Jpn

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“…Change of Cx43 spatial distribution in Sertoli cells from their apical region (3 dpp) to their basal domain (14 dpp) is correlated with the differentiation from a nonpolarized to a polarized epithelium (Vergoween et al, 1991). By the end of the second week post partum, the lumen of the tubule is formed (Flickinger, 1967) and gap junctions (Gilula et al, 1976;Nagano and Suzuki, 1976) and tight-junction protein ZO-1 (Byers et al, 1991) distribution changes from the apical to the basal pole of Sertoli cells. More recently, direct interaction (Giepmans and Moolenaar, 1998) and co-localization of the tight junction protein ZO-1 with Cx43 has been documented (Batias et al, 1999).…”

Section: Bmentioning

confidence: 99%

Expression of Connexin43 in mouse Leydig, Sertoli, and germinal cells at different stages of postnatal development

et al. 2001

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Connexin 43 (Cx43) is the most abundant and ubiquitously distributed gap junction protein in testicular cells. Lack of Cx43 expression results in male infertility. We investigated whether Cx43 is expressed and regulated in Leydig, Sertoli and germinal cells at different stages of postnatal development. Cx43 was detected using three different antibodies shown by immunoblotting to be highly specific. At different postnatal ages Cx43 localization was compared in serial or double labeled testicular cryosections with immunocytochemical distribution of steroidogenic enzyme, 3 ␤hidroxysteroid-dehydrogenase (3␤HSD), Mullerian inhibitory hormone (MIH), and germinal nuclear cell antigen (GNCA1), which are specific markers of interstitial Leydig, Sertoli and germinal cells, respectively. In the interstitium, round cell clumps (RCC) with lipid droplets positive for 3␤HSD and Cx43 were frequently found at intertubular areas at birth and Cx43 was mainly localized at cell membrane appositions. From day 3, the number and size of 3␤HSD-positive RCC started to decrease, and reached a minimum at 7-14 dpp; Cx43 expressed by them is progressively downregulated. From day 21 an increase in the size and number of RCC positive for Cx43 and 3␤HSD was found that continued at 24, 26 and 28 days and reached a maximum at 35 and 60 dpp. Biphasic expression of interstitial Cx43 and 3␤HSD was also found to be positively and temporally correlated with fluctuations in intratesticular testosterone content at all ages studied. In the seminiferous cord (SC), Cx43 was expressed at birth between adjacent Sertoli cells (MIH positive) localized at the periphery, as well as in their cytoplasm projections that surround centrally localized gonocytes. From days 3 to 7, Cx43 labeling increased in Sertoli cells mainly at their apical border. At day 14, Cx43 distribution in Sertoli cells changed from apical to basal in parallel to migration of germinal (GNCA1-positive) cells from the periphery to the center of the SC. At all these ages, Cx43 was also localized at cell borders between Sertoli and germinal cells. In conclusion, this study demonstrates that Cx43 in Leydig cells is regulated during postnatal development in an age and functional dependent manner. In the tubule, it is demonstrated that Cx43 is modulated in Sertoli cells during the neonatal and prepubertal period. We also provide evidence for the first time that Cx43-gap junctions communicate between Sertoli and germinal cells before and during the first wave of spermatogenesis. Anat Rec 264: [13][14][15][16][17][18][19][20][21][22][23][24] 2001.

show abstract

The Sertoli cell occluding junctions and gap junctions in mature and developing mammalian testis

Cited by 300 publications

References 30 publications

Mesaxonal membrane junction of myelinated nerve in the central and peripheral nervous system.

Mesaxonal membrane junction of myelinated nerve in the central and peripheral nervous system.

An Unusual Architecture of Occluding Junctions between Surface Cells in Teleost Ovarian Follicles (<i>Plecoglossus altivelis</i>)

Expression of Connexin43 in mouse Leydig, Sertoli, and germinal cells at different stages of postnatal development

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