The significance of multiplex PCR/heteroduplex analysis‐based TCR‐γ gene rearrangement combined with laser‐capture microdissection in the diagnosis of early mycosis fungoides

Abstract

The strategy of multiple PCR/heteroduplex analysis for TCR-γ gene rearrangement combined with LCM increases the detection rate of clonal TCR-γ gene rearrangement in early MF cases and could provide strong evidence to confirm the diagnosis of early MF.

A specific DNA methylation profile correlates with a high risk of disease progression in stage I classical (Alibert-Bazin type) mycosis fungoides

“…Indeed, tumour and surrounding cells constitute a microenvironment in which interactions and cross‐talks take place, thereby influencing gene expression in all cells . These reciprocal effects may be lost if the analysis is carried out on isolated tumour cells only, providing a partial or distorted view of the tumour biology . In addition, no correlation was found between the lymphoid infiltrate and the prognosis.…”

Determinación de la clonalidad en tejidos humanos

“…También se ha usado electroforesis microcapilar de alta resolución por medio del equipo Agilent 2100 Bioanalyzer (33). Se puede hacer el análisis de fragmentos de doble cadena (formación de heterodupletas) y el de productos de una sola cadena (34,35). Con el fin de inducir la formación de heterodupletas, los productos de la PCR multiplex se someten a calentamiento y enfriamiento rápido (36).…”