The simple and sensitive measurement of malondialdehyde in selected specimens of biological origin and some feed by reversed phase high performance liquid chromatography

Czauderna, M.; Kowalczyk, J.; Marounek, M.

doi:10.1016/j.jchromb.2011.06.008

Cited by 63 publications

(93 citation statements)

References 18 publications

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“…The MDA concentrations in muscle samples were determined after alkaline saponification followed by derivatization with DNPH to MDA-DNPH according to Czauderna et al (2011b). The chromatographic separation of MDA-DNPH from breast muscles was conducted using an ultra-fast liquid chromatography (UFLC) system (SHIMADZU, Japan), incorporating two LC-20ADXP liquid chromatographs (UFLCXR), a SIL-20ACXR auto-sampler (LFLCXR), a CBM-20A communications bus module (UFLC), a CTO-20A column oven, a DGU-20A5 degasser, and a SPD photodiode array detector (DAD).…”

Section: Preparation and Derivatisation Of Mda In Breast Muscle Samplmentioning

confidence: 99%

“…The column was a Phenomenex C 18 -column (Synergi 2.5 µm, Hydro-RP, 100 Å, 100 mm in length) with an inner diameter of 2 mm. MDA in processed biological samples was analysed using a linear gradient of acetonitrile in water and the photodiode detector was set to 307 nm for UV detection (Czauderna et al, 2011b). The concentration of MDA was calculated based on the fresh weight of breast muscle samples.…”

Section: Preparation and Derivatisation Of Mda In Breast Muscle Samplmentioning

confidence: 99%

“…Indeed, our proposed original indicator of PUFA peroxidation (MDA index ; Table 6) takes into consideration both PUFA and MDA concentrations in the examined muscles. Indeed, the MDA yield depends on the concentration of PUFA in the analysed tissues (Del Rio et al, 2005;Czauderna et al, 2011b;). Moreover, the presence of antioxidants (like Se-compounds or tocopherols) can reduce MDA formation in animals and humans (Schweizer et al, 2005;Czauderna et al, 2011b).…”

Section: Effect Of the Experimental Rations On The Yield Of Pufa Peromentioning

confidence: 99%

“…Indeed, the MDA yield depends on the concentration of PUFA in the analysed tissues (Del Rio et al, 2005;Czauderna et al, 2011b;). Moreover, the presence of antioxidants (like Se-compounds or tocopherols) can reduce MDA formation in animals and humans (Schweizer et al, 2005;Czauderna et al, 2011b). According to the above and all of the results summarized in Table 6, we argue that our proposed original MDA index (calculated as: MDA index = (MDA+PUFA)/PUFA); Table 6) is the best indicator of the yield of PUFA peroxidation in tissues of living organisms.…”

Section: Effect Of the Experimental Rations On The Yield Of Pufa Peromentioning

confidence: 99%

“…One of the main mechanisms by which meat quality deteriorates is lipid oxidation (Wood et al, 2003;Rey et al, 2004), which is manifested by adverse changes in flavour, colour, texture, and nutritive value and by the possible production of toxic compounds (mainly malondialdehydea biomarker of lipid peroxidation) (Del Rio et al, 2005;Czauderna et al, 2011b). Lipid oxidation depends on a number of factors, including the polyunsaturated fatty acid (PUFA) content, and the concentration of prooxidants and antioxidants (Rey et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Dietary lycopenes, selenium compounds and fish oil affect the profile of fatty acids and oxidative stress in chicken breast muscle

et al. 2012

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The aim of the study was to determine the influence of the addition for 6 weeks of 12 ppm lycopene (Lyc), 2% fish oil (FO) and/or 0.25 ppm Se as selenate (Se VI ) or selenized yeast (SeY) to an isoenergetic and isonitrogenous basal ration containing sunflower oil (SO) as the source of energy on the liveweight (LW) and feed intake per kg body weight gain of pullets and cockerels. The effects of these additives on the concentrations of fatty acids (FA), especially mono-(MUFA) and polyunsaturated (PUFA) acids, and the yield of PUFA peroxidation (oxidative stress) in breast muscles of chickens were also investigated. Feeding broilers with the experimental rations slightly increased the live body weight of chickens compared with the controls. The ration containing SeY, Lyc, and FO most effectively increased the LW of cockerels, while that of pullets was most efficiently increased by the ration enriched in SeY and Lyc, irrespective of the presence of extra FO. SeY in the ration with Lyc most efficiently elevated feed conversion efficiency (FCE), while Se VI in the ration containing Lyc most effectively decreased FCE. The addition of Se VI to the ration enriched in Lyc significantly elevated the sum of saturated fatty acids (SFA), MUFA, and PUFA in muscles of pullets and, especially, cockerels in comparison with all other examined groups. In contrast, FO added to the ration containing Lyc, irrespective of the presence of Se VI or SeY, decreased the muscle sum of SFA, MUFA, and PUFA, and increased the concentration of docosahexaenoic acid in muscles of chickens compared with the control and all other experimental groups. FO added to the ration, regardless of the presence of Se VI or SeY, elevated the value of the long-chain n-3 PUFAto-SFA ratio and significantly decreased the values of the n-6 PUFA/n-3 PUFA ratio in muscles of pullets and cockerels. The ration containing Lyc and Se VI most efficiently increased the value of the 3 Corresponding author: e-mail: m.czauderna@ifzz.pan.pl Journal of Animal and Feed Sciences, 21, 2012, 705-724 706 LYCOPENES AND Se COMPOUNDS -FA IN CHICKEN MUSCLE PUFA/SFA ratio in the muscles of pullets and, especially, cockerels. The ration containing FO and Lyc most significantly increased the accumulation of malondialdehyde (MDA) in muscles of pullets and, in particular, cockerels, while addition of Se VI or SeY to the ration enriched in FO and Lyc most considerably decreased the concentration of MDA in breast muscles compared with the control and all other experimental groups. We propose an MDA index (calculated as: MDAindex = (MDA+PUFA)/ PUFA) as an original indicator of PUFA peroxidation in tissues of living organisms.Our results of feeding diets with Lyc, FO, and Se as Se VI or SeY constitute useful information for nutritionists carrying out investigations to improve chicken performance and the nutritional quality of feed, as well as to improve the nutritive value of chicken meat from the aspect of human health.

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Section: Preparation and Derivatisation Of Mda In Breast Muscle Samplmentioning

confidence: 99%

Section: Preparation and Derivatisation Of Mda In Breast Muscle Samplmentioning

confidence: 99%

Section: Effect Of the Experimental Rations On The Yield Of Pufa Peromentioning

confidence: 99%

Section: Effect Of the Experimental Rations On The Yield Of Pufa Peromentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dietary lycopenes, selenium compounds and fish oil affect the profile of fatty acids and oxidative stress in chicken breast muscle

et al. 2012

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Quantification of Malondialdehyde in Human Urine by HPLC‐DAD and Derivatization with 2,4‐Dinitrophenylhydrazine

Kim

Jung

Kho

2017

Bulletin Korean Chem Soc

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This study demonstrated that human urine can be derivatized in a simple manner and the proposed method can be suitable for the routine and noninvasive evaluation of oxidative stress. A rapid and sensitive method for the determination of malondialdehyde (MDA) concentrations in human urine has been developed using high‐performance liquid chromatography. The MDA concentration in pooled human urine samples was determined after its conversion with 2,4‐dinitrophenylhydrazine (DNPH) to a hydrazone (MDA‐DNPH). The MDA‐DNPH was separated on C18 column and quantified by diode array detection at 310 nm. The method was validated by the use of spiked human urine. The calibration curves of analyte showed good linearity (r 2 > 0.999) in the ranges. The limit of detection was estimated as 0.16 µg/mL. In addition, accuracy was from 104.0 to 112.7% and the precision was less than 3.2%. In this study, the developed method was applied to the analysis of real human urine samples. The level of MDA in urine of adults was 1.3 ± 0.6 µg/mg creatinine.

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Modeling and optimization of malondialdehyde (MDA) absorbance behavior through response surface methodology (RSM) and artificial intelligence network (AIN): An endeavor to estimate lipid peroxidation by determination of MDA

Akbari

Najafi

Bahmaie

et al. 2023

Journal of Chemometrics

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Oxidative stress (OS) associated with reactive oxygen species (ROS) attacks many biomolecules, leading to cell death, lipid peroxidation (LP), and physiological mechanism damage in the human body because of its reaction with nucleotides, membrane lipids, and proteins. Malondialdehyde (MDA) is one of the end products of polyunsaturated fatty acids peroxidation. The elevated level of MDA is widely known as a biomarker for the indirect measurement of OS during LP. Although several derivatizations' approaches and successful commercial kits are available, their analytical validity and reliability in clinical studies require further attention to obtain robust quantitation and high‐quality data. Also, re‐evaluation and development of new analytical methods with high sensitivity and selectivity in response to free MDA and total MDA, DNA and protein adducts are not well‐characterized. Here, response surface methodology (RSM) and artificial neural network (ANN) were proposed to model, predict, and optimize MDA detection. This paper introduced a novel, simple, and fast modeling method using a new reagent (para methoxy aniline or PMA) for the first time to convert MDA to an adduct which could be detectable by UV‐Vis spectroscopy. Based on the interactions between MDA and PMA at times and temperatures, RSM and artificial intelligence network (AIN) were then applied to forecast the absorbance rate of MDA‐PMA. The optimum mode for the highest absorbance rate of MDA‐PMA is at the temperature of 74.99°C with a PMA concentration of 499.87 μM and MDA concentration of 49.98 μM. The best structure with five hidden layers, including 5,5,5,9,4 neurons with the transfer function logsig for each layer, was selected for the MLP‐ANN model. The maximum error rate of the MLP‐ANN model was 2.08%.Our new approach is an improvement over existing assay kits, overcoming limitations such as time, temperature, and reliability with a bright future to target increased MDA level in human samples.

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The simple and sensitive measurement of malondialdehyde in selected specimens of biological origin and some feed by reversed phase high performance liquid chromatography

Cited by 63 publications

References 18 publications

Dietary lycopenes, selenium compounds and fish oil affect the profile of fatty acids and oxidative stress in chicken breast muscle

Dietary lycopenes, selenium compounds and fish oil affect the profile of fatty acids and oxidative stress in chicken breast muscle

Quantification of Malondialdehyde in Human Urine by HPLC‐DAD and Derivatization with 2,4‐Dinitrophenylhydrazine

Modeling and optimization of malondialdehyde (MDA) absorbance behavior through response surface methodology (RSM) and artificial intelligence network (AIN): An endeavor to estimate lipid peroxidation by determination of MDA

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