The single cellular green microalga Botryococcus braunii, race B possesses three distinct 1-deoxy-d-xylulose 5-phosphate synthases

Matsushima, Daisuke; Jenke‐Kodama, Holger; Sato, Yoshihito; Fukunaga, Yusuke; Sumimoto, Koremitsu; Kuzuyama, Tomohisa; Matsunaga, Shigeki; Okada, Shigeru

doi:10.1016/j.plantsci.2012.01.002

Cited by 40 publications

(35 citation statements)

References 54 publications

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“…While multiple isozymes of DXS are routinely found in plants [60], genomic evidence shows that strains of green algae harbor only a single DXS each. Recently, biochemical characterization of three isozymes of DXS from race B demonstrated that all three isozymes are active and have similar kinetic parameters [61]. Our data also indicate similar, moderate transcript abundances for all three DXS isozymes of the race B strain.…”

Section: Transcriptome Analysis Of B Brauniisupporting

confidence: 68%

Review of the algal biology program within the National Alliance for Advanced Biofuels and Bioproducts

et al. 2017

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In 2010, when the National Alliance for Advanced Biofuels and Bioproducts (NAABB) consortium began, little was known about the molecular basis of algal biomass or oil production. Very few algal genome sequences were available and efforts to identify the best-producing wild species through bioprospecting approaches had largely stalled after the U.S. Department of Energy's Aquatic Species Program. This lack of knowledge included how reduced carbon was partitioned into storage products like triglycerides or starch and the role played by metabolite remodeling in the accumulation of energy-dense storage products. Furthermore, genetic transformation and metabolic engineering approaches to improve algal biomass and oil yields were in their infancy. Genome sequencing and transcriptional profiling were becoming less expensive, however; and the tools to annotate gene expression profiles under various growth and engineered conditions were just starting to be developed for algae. It was in this context that an integrated algal biology program was introduced in the NAABB to address the greatest constraints limiting algal biomass yield. This review describes the NAABB algal biology program, including hypotheses, research objectives, and strategies to move algal biology research into the twenty-first century and to realize the greatest potential of algae biomass systems to produce biofuels.

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Section: Transcriptome Analysis Of B Brauniisupporting

confidence: 68%

Review of the algal biology program within the National Alliance for Advanced Biofuels and Bioproducts

et al. 2017

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“…Race B uses the methylerythritol phosphate (MEP) pathway to produce universal precursors for isoprenoids, and three genes that encode enzymes involved in this pathway have been cloned from race B [20]. In addition, the genes that encode key enzymes (squalene synthase like enzymes; SSL-1, 2, and 3) that catalyze the final steps of the transformation from farnesyl diphosphate to C 30 botryococcene (SSL-1 and 3) and squalene (SSL-1 and 2) have been identified [21].…”

Section: Introductionmentioning

confidence: 99%

Colony sheath formation is accompanied by shell formation and release in the green alga Botryococcus braunii (race B)

et al. 2015

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a b s t r a c tBotryococcus braunii race B is a promising candidate for renewable biofuel production. Its colony organization is unique. Cells are connected by extracellular biopolymers containing hydrocarbons at their basolateral regions, enclosed by a retaining wall, and form a sphere inside a colony sheath composed of polysaccharide fibrils. This unique colony structure is a key for the hydrocarbon production and storage, but how the colony forms remains unknown. We studied ultrastructure of colony sheath and its formation process by rapid freezing and freezesubstitution electron microscopy. Fibrils of colony sheath extend from the retaining wall and are branched, and repel India ink. The sheath formation starts in mitosis and completes during the following daughter cell maturation. In this process, the older retaining wall and thin layers at the cell apex are released as shells and new fibrils formed on the new retaining wall extend into and merge with the pre-existing colony sheath. Its formation also accompanied by the hydrocarbon secretion and accumulation at the basolateral region. The fibrils of colony sheath that extend from a cell wall are replaced by new fibrils after each cell division, while fibrils in the extracellular biopolymers accumulate to form the remaining part of sheath, without replacement.

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“…Race A produces alkadienes and alkatrienes (14), race B produces mainly triterpenoids known as botrococcenes (15,16), and race L produces a tetraterpenoid known as lycopadiene (17). In both races A and B, the biosynthetic pathways to these hydrocarbons have been clarified, and some of the enzymes involved in the synthetic activity have been characterized (18)(19)(20)(21)(22)(23)(24). In addition, the associated patterns of gene expression have been examined to clarify the biosynthetic pathways to hydrocarbons (25)(26)(27).…”

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confidence: 99%

Active Hydrocarbon Biosynthesis and Accumulation in a Green Alga, Botryococcus braunii (Race A)

et al. 2013

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The single cellular green microalga Botryococcus braunii, race B possesses three distinct 1-deoxy-d-xylulose 5-phosphate synthases

Cited by 40 publications

References 54 publications

Review of the algal biology program within the National Alliance for Advanced Biofuels and Bioproducts

Review of the algal biology program within the National Alliance for Advanced Biofuels and Bioproducts

Colony sheath formation is accompanied by shell formation and release in the green alga Botryococcus braunii (race B)

Active Hydrocarbon Biosynthesis and Accumulation in a Green Alga, Botryococcus braunii (Race A)

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