The small GTPase Rhb1 is involved in the cell response to fluconazole in<i>Candida albicans</i>

Chen, Yu‐Wen; Yeh, Ying-Chieh; Chen, Hsueh‐Fen; Chen, Ruei-Ching; Lin, Guan‐Yu; Chen, Yuting; Lan, Chung-Yu

doi:10.1093/femsyr/foz005

Cited by 3 publications

(4 citation statements)

References 41 publications

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“…Protein extraction and western blotting were performed as previously described [ 30 ]. An anti-phospho-p38 (Thr180/Tyr182) monoclonal antibody (#9211, Cell Signaling Technology, Danvers, MA, USA) and Hog1 (y-215) antibody (sc-9079, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) were used to detect phospho-Hog1 and total Hog1, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, C. albicans cells were diluted to a final concentration of 3 × 10 8 CFU/mL with 12.5 mM sodium acetate, incubated with or without different concentrations of LfcinB15 at 37 °C for 1 h, and collected by centrifugation. The supernatant was kept on ice before use for measuring the extracellular ATP level, while the cell pellets were broken, as previously described [ 30 ], for measuring intracellular ATP.…”

Section: Methodsmentioning

confidence: 99%

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Antimicrobial Activity of the Peptide LfcinB15 against Candida albicans

Chang

Kao

Lan

2021

JoF

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Lactoferricin (Lfcin) is an amphipathic, cationic peptide derived from proteolytic cleavage of the N-lobe of lactoferrin (Lf). Lfcin and its derivatives possess broad-spectrum antibacterial and antifungal activities. However, unlike their antibacterial functions, the modes of action of Lfcin and its derivatives against pathogenic fungi are less well understood. In this study, the mechanisms of LfcinB15, a derivative of bovine Lfcin, against Candida albicans were, therefore, extensively investigated. LfcinB15 exhibited inhibitory activity against planktonic cells, biofilm cells, and clinical isolates of C. albicans and non-albicans Candida species. We further demonstrated that LfcinB15 is localized on the cell surface and vacuoles of C. albicans cells. Moreover, LfcinB15 uses several different methods to kill C. albicans, including disturbing the cell membrane, inducing reactive oxygen species (ROS) generation, and causing mitochondrial dysfunction. Finally, the Hog1 and Mkc1 mitogen-activated protein kinases were both activated in C. albicans cells in response to LfcinB15. These findings help us to obtain more insight into the complex mechanisms used by LfcinB15 and other Lfcin-derived peptides to fight fungal pathogens.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Antimicrobial Activity of the Peptide LfcinB15 against Candida albicans

Chang

Kao

Lan

2021

JoF

Self Cite

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“…In order to understand the mitochondrial energetic metabolism of C. albicans treated with C18, intracellular ATP levels was measured using ATP assay kits (Beyotime China) according to the manufacturer's instructions (Chen Y. W. et al, 2019). Briefly, 2.0 × 10 6 CFU/ml of C. albians on logarithmic phrase in YPD broth was exposed to 32 µg/ml and 64 µg/ml C18 for 3 h, 6 h, and 12 h at 35 • C. After harvest, the cells were washed twice in sterile PBS and adjusted to 2 × 10 6 CFU/ml in YPD medium.…”

Section: Detection Of Intracellular Adenosine Triphosphate Levelsmentioning

confidence: 99%

A Cecropin-4 Derived Peptide C18 Inhibits Candida albicans by Disturbing Mitochondrial Function

et al. 2022

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Global burden of fungal infections and related health risk has accelerated at an incredible pace, and multidrug resistance emergency aggravates the need for the development of new effective strategies. Candida albicans is clinically the most ubiquitous pathogenic fungus that leads to high incidence and mortality in immunocompromised patients. Antimicrobial peptides (AMPs), in this context, represent promising alternatives having potential to be exploited for improving human health. In our previous studies, a Cecropin-4-derived peptide named C18 was found to possess a broader antibacterial spectrum after modification and exhibit significant antifungal activity against C. albicans. In this study, C18 shows antifungal activity against C. albicans or non-albicans Candida species with a minimum inhibitory concentration (MIC) at 4∼32 μg/ml, and clinical isolates of fluconazole (FLZ)-resistance C. tropicalis were highly susceptible to C18 with MIC = 4∼32 μg/ml. Additionally, C18 is superior to FLZ for killing planktonic C. albicans from inhibitory and killing kinetic curves. Moreover, C18 could attenuate the virulence of C. albicans, which includes damaging the cell structure, retarding hyphae transition, and inhibiting biofilm formation. Intriguingly, in the Galleria mellonella model with C. albicans infection, C18 could improve the survival rate of G. mellonella larvae to 70% and reduce C. albicans load from 5.01 × 107 to 5.62 × 104 CFU. For mechanistic action of C18, the level of reactive oxygen species (ROS) generation and cytosolic Ca2 + increased in the presence of C18, which is closely associated with mitochondrial dysfunction. Meanwhile, mitochondrial membrane potential (△Ψm) loss and ATP depletion of C. albicans occurred with the treatment of C18. We hypothesized that C18 might inhibit C. albicans via triggering mitochondrial dysfunction driven by ROS generation and Ca2 + accumulation. Our observation provides a basis for future research to explore the antifungal strategies and presents C18 as an attractive therapeutic candidate to be developed to treat candidiasis.

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“…We selected 28 genes with SNPs that are known to be important for antifungal resistance phenotype in human pathogenic fungi such as Candida albicans (Chen et al, 2019;Feng et al, 2020;Feng et al, 2017;Ramirez-Zavala et al, 2018;Whaley et al, 2016;Zhang et al, 2015a), C. tropicalis (Tan et al, 2015), C. glabrata (Ahmad et al, 2019;Arastehfar et al, 2019;Culakova et al, 2015;Delliere et al, 2016;Hou et al, 2019;Whaley et al, 2018), C. parapsilosis (Rybak et al, 2017), C. dubliniensis (Asadzadeh et al, 2017), Saccharomyces cerevisiae (Chen et al, 2014), Cryptococcus spp. (Billmyre et al, 2020;Son et al, 2018), Aspergillus spp.…”

Section: Characterization Of Point Mutations and Snps In S Brasiliens...mentioning

confidence: 99%

Single nucleotide polymorphisms and chromosomal copy number variation may impact the Sporothrix brasiliensis antifungal susceptibility and sporotrichosis clinical outcomes

Teixeira¹,

Almeida‐Paes²,

Bernardes-Engemann³

et al. 2022

Fungal Genetics and Biology

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The small GTPase Rhb1 is involved in the cell response to fluconazole inCandida albicans

Cited by 3 publications

References 41 publications

Antimicrobial Activity of the Peptide LfcinB15 against Candida albicans

Antimicrobial Activity of the Peptide LfcinB15 against Candida albicans

A Cecropin-4 Derived Peptide C18 Inhibits Candida albicans by Disturbing Mitochondrial Function

Single nucleotide polymorphisms and chromosomal copy number variation may impact the Sporothrix brasiliensis antifungal susceptibility and sporotrichosis clinical outcomes

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