The Soil Microbiome Reduces Striga Infection of Sorghum by Modulation of Host-Derived Signaling Molecules and Root Development

Kawa, Dorota; Thiombiano, Benjamin; Shimels, Mahdere; Taylor, Tamera; Walmsley, Aimee; Vahldick, Hannah; Leite, Márcio Fernandes Alves; Musa, Zayan; Bucksch, Alexander; Dini‐Andreote, Francisco; Chen, Alexander J.; Daksa, Jiregna; Etalo, Desalegn W.; Tessema, Taye; Kuramae, E.E.; Raaijmakers, J.M.; Bouwmeester, Harro J.; Brady, Siobhán M.

doi:10.2139/ssrn.4350137

Cited by 4 publications

(2 citation statements)

References 73 publications

(86 reference statements)

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“…Indeed, several studies claimed that the suppressiveness of Orobanche sp. as well as Striga hermonthica is associated with the presence of specific bacterial taxa, including Pseudomonas (Kawa et al, 2022; Zermane et al, 2007). Since, PGC‐producing Pseudomonas can be followed in soil via qPCR approaches (Almario et al, 2013), determining the correlation between the community of PGC‐producing Pseudomonas and the level of parasitic plant infection would bring new insights on the ecological role of these bacteria in suppressive soils.…”

Section: Discussionmentioning

confidence: 99%

“…This constitute the main problem to constrain their deleterious impact on crops (Haring & Flessner, 2018). The survival of the seeds depends on various abiotic factors (pH, humidity, climate) (Rubiales et al, 2003) and biotic factors (host plants, soil and rhizosphere microbiota; (Huet et al, 2020; Kawa et al, 2022; Martinez et al, 2023; Mutuku et al, 2021). Different agricultural strategies attempt to regulate broomrape populations in agroecosystems, such as crop rotation, triggering the suicidal germination of the plant parasitic seeds or the use of resistant host plant varieties or chemical herbicides (Cartry et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of broomrape germination by 2,4‐diacetylphloroglucinol produced by environmental Pseudomonas

Lurthy,

Perot,

Gerin‐Eveillard

et al. 2023

Microbial Biotechnology

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Parasitic weeds such as broomrapes (Phelipanche ramosa and Orobanche cumana) cause severe damage to crops and their development must be controlled. Given that phloroglucinol compounds (PGCs) produced by environmental Pseudomonas could be toxic towards certain plants, we assessed the potential herbicidal effect of the bacterial model Pseudomonas ogarae F113, a PGCs‐producing bacterium, on parasitic weed. By combining the use of a mutagenesis approach and of pure PGCs, we evaluated the in vitro effect of PGC‐produced by P. ogarae F113 on broomrape germination and assessed the protective activity of a PGC‐producing bacteria on oilseed rape (Brassica napus) against P. ramosa in non‐sterile soils. We showed that the inhibition of the germination depends on the PGCs molecular structure and their concentrations as well as the broomrape species and pathovars. This inhibition caused by the PGCs is irreversible, causing a brown coloration of the broomrape seeds. The inoculation of PGCs‐producing bacteria limited the broomrape infection of P. ramosa, without affecting the host growth. Moreover, elemental profiling analysis of oilseed rape revealed that neither F113 nor applied PGCs affected the nutrition capacity of the oilseed rape host. Our study expands the knowledge on plant‐beneficial Pseudomonas as weed biocontrol agents and opens new avenues for the development of natural bioherbicides to enhance crop yield.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inhibition of broomrape germination by 2,4‐diacetylphloroglucinol produced by environmental Pseudomonas

Lurthy,

Perot,

Gerin‐Eveillard

et al. 2023

Microbial Biotechnology

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Transcriptome atlas of Striga germination: Implications for managing an intractable parasitic plant

Irafasha

Mutinda

Mobegi

et al. 2023

Plants People Planet

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Societal Impact StatementWitchweeds, parasitic plants of the genus Striga, are nicknamed “cereal killers” because of their devastating destruction of Africa's most staple cereals, including maize, sorghum, millets, and upland rice. The parasite relies on biomolecules emitted from the host roots to germinate and therefore initiate its infectious lifecycle. Some sorghum varieties have evolved to not produce effective germination stimulants, making them resistant to the parasite. Here, the genetic factors that underpin Striga germination were assessed, followed by a discussion of how such knowledge can be used to develop new Striga management strategies through the disruption of host–parasite communication exchange.Summary Seeds of the parasitic plant Striga are dormant. They only germinate in response to biomolecules emitted from the host's root exudate, strigolactones (SL). But it is now emerging that Striga germination is a much more complex process regulated by crosstalk of hormone signaling pathways. To further understand the genetic basis of the communication exchange between Striga and its host sorghum, we performed a comparative transcriptomic analysis. We sought to identify major transcriptomic changes that define the germination process in Striga and a set of genes that may contribute to the differences in germination rates. Results showed that germination proceeds immediately after SL perception and is marked by a wave of transcriptional reprogramming to allow for metabolic processes of energy mobilization. Cluster analysis using self‐organizing maps revealed a time‐phased and genotype‐differentiated response to germination stimulation. The variation in germination was also a function of hormonal crosstalk. The early germination stage was associated with significant repression of genes in the abscisic acid (ABA) biosynthesis pathway. Other hormones influenced germination as follows: (i) ABA and auxin repressed germination; (ii) brassinosteroid, ethylene, and jasmonic acid promoted germination; and (iii) cytokinin had a more prominent role post‐germination rather than during germination. Perception of SL sets the germination program leading to different rates of germination in sorghum, followed by a complex hormonal regulation network that acts to either repress or enhance germination. We discuss the implications of these findings and present new plausible Striga management strategies.

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The role of strigolactone structural diversity in the host specificity and control of Striga, a major constraint to sub‐Saharan agriculture

Shimels,

Rendine,

Ruyter‐Spira

et al. 2024

Plants People Planet

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Social Impact StatementThe parasitic weed Striga affects crops such as sorghum, maize, millet, and rice in over 40 countries on the African continent and negatively impacts the livelihood of over 300 million small‐holder farmers. Striga seeds can remain dormant in the soil for many years until they are triggered to germinate by germination stimulants, called strigolactones, exuded from the roots of their host. Here, the current knowledge on the biosynthesis of the strigolactones, their structural diversity, and biological relevance are reviewed. This knowledge could improve Striga control and thus improve the livelihood of small‐holder farmers.SummaryThe parasitic plant genus Striga causes major yield losses to several crops such as sorghum, millet, and rice in arid and semi‐arid regions of the tropics. For Striga to successfully parasitize its host plant, two conditions should be fulfilled: suitable germination conditions and the presence of a host plant that exudes so‐called germination stimulants, strigolactones, that are also as a signal to attract beneficial micro‐organisms such as arbuscular mycorrhizal (AM) fungi. Different plant species exude qualitatively and quantitatively different blends of strigolactones, and this plays a key role in determining Striga host specificity. Sorghum lgs1 genotypes with a mutation in a sulfotransferase (SbSOT4A), for example, exude orobanchol and are resistant to Striga, while 5‐deoxystrigol is the major strigolactone exuded by susceptible cultivars with wild type SbSOT4A. In this review, we discuss the current knowledge on the biosynthesis of the large diversity of strigolactones, how SbSOT4A may be involved in this, and how strigolactone diversity may contribute to microbiome recruitment. Finally, we discuss how knowledge on the importance of strigolactone diversity can contribute to Striga control.

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The Soil Microbiome Reduces Striga Infection of Sorghum by Modulation of Host-Derived Signaling Molecules and Root Development

Cited by 4 publications

References 73 publications

Inhibition of broomrape germination by 2,4‐diacetylphloroglucinol produced by environmental Pseudomonas

Inhibition of broomrape germination by 2,4‐diacetylphloroglucinol produced by environmental Pseudomonas

Transcriptome atlas of Striga germination: Implications for managing an intractable parasitic plant

The role of strigolactone structural diversity in the host specificity and control of Striga, a major constraint to sub‐Saharan agriculture

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