The Sphinx That Traveled to Philadelphia

Wegner, Josef; Wegner, Jennifer Houser

doi:10.9783/9781934536773

Cited by 3 publications

(3 citation statements)

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“…The ligand exchange rate of Co(III) is slow, essentially locking down HA2* through hexa-His tag binding. 18,19 Wells testing neutral pH (prefusion) are incubated with 300 μL of assay buffer for 1 h. Wells testing low pH (postfusion) are incubated with dilute HCl (1 mM). After incubation, wells are washed three times with assay buffer.…”

Section: Antibody Bindingmentioning

confidence: 99%

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Stability of HA2 Prefusion Structure and pH-Induced Conformational Changes in the HA2 Domain of H3N2 Hemagglutinin

2021

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Influenza hemagglutinin is the fusion protein that mediates fusion of the viral and host membranes through a large conformational change upon acidification in the developing endosome. The "spring-loaded" model has long been used to describe the mechanism of hemagglutinin and other type 1 viral glycoproteins. This model postulates a metastable conformation of the HA2 subunit, caged from adopting a lower-free energy conformation by the HA1 subunit. Here, using a combination of biochemical and spectroscopic methods, we study a truncated construct of HA2 (HA2*, lacking the transmembrane domain) recombinantly expressed in Escherichia coli as a model for HA2 without the influence of HA1. Our data show that HA2* folds into a conformation like that of HA2 in full length HA and forms trimers. Upon acidification, HA2* undergoes a conformational change that is consistent with the change from pre-to postfusion HA2 in HA. This conformational change is fast and occurs on a time scale that is not consistent with aggregation. These results suggest that the prefusion conformation of HA2 is stable and the change to the postfusion conformation is due to protonation of HA2 itself and not merely uncaging by HA1.

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Section: Antibody Bindingmentioning

confidence: 99%

“…HA2* bound to Co 2+ becomes essentially irreversibly bound when Co 2+ is oxidized to Co 3+ . 18,19 This allows unbound HA2* to be thoroughly washed away without appreciably reducing the amount of HA2* bound to the surface.…”

Section: Antibody Bindingmentioning

confidence: 99%

Stability of HA2 Prefusion Structure and pH-Induced Conformational Changes in the HA2 Domain of H3N2 Hemagglutinin

2021

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“…The text was described as ''one of the most sought-after reference works for every history of anatomy enthusiast.'' 19 This was the book which Frank chose to translate and annotate. Frank commented that this important resource had been out of print for many years, and due to increasing interest in the subject and the many new discoveries made by subsequent scholars in this subject since 1852, Frank thought it appropriate to both translate and update the text.…”

Section: Frank's Translation Of Choulant's Textmentioning

confidence: 99%

Mortimer Frank, Johann Ludwig Choulant, and the history of anatomical illustration

Feibel

2018

J Med Biogr

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Mortimer Frank (1874–1919) was an ophthalmologist in Chicago, Illinois. He published a number of papers on the history of medicine, and was secretary of the Chicago Society of the History of Medicine and editor of their Bulletin. His major contribution to the history of medicine relates to the history of anatomical illustration. The classic book on that subject had been published in 1852 in German by the physician and historian, Johann Ludwig Choulant (1791–1861). However, by Frank’s time this text was both out dated and out of print. Frank took on the tremendous project of translating Choulant’s German text into English as History and Bibliography of Anatomic Illustration in Its Relation to Anatomic Science and The Graphic Arts. He improved Choulant’s text with the results of his and other scholars’ research, greatly enlarging the text. Frank supplemented the original book with a biography of Choulant, essays on anatomists not considered in the original text, and an essay on the history of anatomical illustration prior to those authors discussed by Choulant. This book, now referred to as Choulant/Frank, has been reprinted several times, and is still useful as a reference in this field, though some of its research is now dated.

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2016

The American Historical Review

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The Sphinx That Traveled to Philadelphia

Cited by 3 publications

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Stability of HA2 Prefusion Structure and pH-Induced Conformational Changes in the HA2 Domain of H3N2 Hemagglutinin

Stability of HA2 Prefusion Structure and pH-Induced Conformational Changes in the HA2 Domain of H3N2 Hemagglutinin

Mortimer Frank, Johann Ludwig Choulant, and the history of anatomical illustration

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