The Spice Sage and Its Active Ingredient Rosmarinic Acid Protect PC12 Cells from Amyloid-β Peptide-Induced Neurotoxicity

Iuvone, Teresa; Filippis, Daniele De; Esposito, Giuseppe; D’Amico, Alessandra; Izzo, Angelo A.

doi:10.1124/jpet.105.099317

Cited by 222 publications

(135 citation statements)

References 39 publications

Supporting

Mentioning

124

Contrasting

Unclassified

Order By: Relevance

“…In the current study, increase in brain TBARS and hippocampus 4-HNE levels in the OD group supports previous findings [44,45] that show the importance of lipid peroxidation in the development of AD. In support of previous cell culture study [46], RA also decreased lipid peroxidation in our study. In addition, RA treatment attenuated 4-HNE level in the ODR rats.…”

Section: Discussionsupporting

confidence: 78%

Effects of rosmarinic acid on cognitive and biochemical alterations in ovariectomized rats treated with D-galactose

Kantar

Ozturk

et al. 2016

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

Introduction. Animal models designed to mimic certain features of Alzheimer's disease (AD) can help us to increase our understanding of the underlying mechanisms of disease. Previous studies have revealed that long-term D-galactose injection combined with ovariectomy results in pathophysiologic alterations associated with AD. Thus, the aim of the present study was to investigate the effects of rosmarinic acid (RA) administration on pathological changes associated with ovariectomy and D-galactose injection, which serve as a two-insult model for AD. Material and methods. One hundred female Wistar rats were divided into five equal groups: control (C), Sham (Sh), rosmarinic acid treated (R), ovariectomized rats treated with D-galactose (OD), ovariectomized rats treated with D-galactose and rosmarinic acid (ODR) groups. D-galactose (80 mg/kg/day) was administered by i.p. injection and RA (50 mg/kg/day) was given via gavage for 60 days. Open field and Y-maze tests were used to assess locomotor activity and short-term spatial memory, respectively. Biochemical and histopathological analyses of the brain tissue were performed. Results. Open field testing showed that the locomotor activity and exploratory behavior of rats were prominently impaired in the OD group as compared to the other studied groups. Similarly, Y-maze test results revealed a decrease of short-term spatial memory in the OD rats. A concomitant treatment with RA significantly restored altered locomotor activity and cognitive functions in the ODR group. Lipid peroxidation levels, cyclooxygenase-2 expression and prostaglandin E2 levels in the brain tissue were higher in the OD group and RA treatment inhibited these changes. AD-like histopathological alterations and amyloid b peptide (Ab) depositions were observed in the OD group. Normal cell structure and lower Ab depositions were observed in the ODR group compared with the OD group. Conclusions. RA could have the potential to prevent some psychological and biochemical alterations of brain tissue found in a rat model of AD probably by attenuating lipid peroxidation and inflammatory response.

show abstract

Section: Discussionsupporting

confidence: 78%

Effects of rosmarinic acid on cognitive and biochemical alterations in ovariectomized rats treated with D-galactose

Kantar

Ozturk

et al. 2016

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

show abstract

“…Interestingly, Ono and coworkers [62] reported that RA inhibited the formation of fibrils from Aβ and destabilized preformed Aβ fibrils in vitro. Furthermore, Iuvone and coworkers [63] demonstrated that RA is able to inhibit tau hyperphosphorylation, probably through the inhibition of the p38 MAP kinase pathway but not via the inhibition of GSK-3 hyperphosphorylation. RA inhibited caspase-3 activation and DNA fragmentation, thus suggesting that RA could affect the execution phase of Aβ-induced apoptosis [63].…”

Section: Rosmarinic Acidmentioning

confidence: 99%

“…Furthermore, Iuvone and coworkers [63] demonstrated that RA is able to inhibit tau hyperphosphorylation, probably through the inhibition of the p38 MAP kinase pathway but not via the inhibition of GSK-3 hyperphosphorylation. RA inhibited caspase-3 activation and DNA fragmentation, thus suggesting that RA could affect the execution phase of Aβ-induced apoptosis [63]. The biochemical mechanisms related to RA are summarized in l " Table 9.…”

Section: Rosmarinic Acidmentioning

confidence: 99%

Effects of Salvia miltiorrhiza on CNS Neuronal Injury and Degeneration: A Plausible Complementary Role of Tanshinones and Depsides

Bonaccini

Karioti²,

Bergonzi

et al. 2015

Planta Med

View full text Add to dashboard Cite

show abstract

“…The ability of chlorogenic acid (20 and 50 μM) to protect the PC12 cells from different concentrations of Aβ (1, 5, 10, 25 and 50 μM) was also determined using a MTT assay, as described above. Rosmarinic acid was used as the positive control (Iuvone et al, 2006;Park et al, 2009). …”

Section: Determination Of the Ability Of Chlorogenic Acid To Protect mentioning

confidence: 99%

Protective Effect of Chlorogenic Acid against Aβ-Induced Neurotoxicity

Lee¹,

Won²,

Kim³

et al. 2011

Biomolecules and Therapeutics

View full text Add to dashboard Cite

Beta-amyloid (Aβ) is considered as one of the major causes of Alzheimer's disease. This study examined the neuroprotective effects of chlorogenic acid, a naturally occurring polyphenol which is distributed widely in plants, fruits and vegetables, against Aβ-induced toxicity. Aβ decreased signifi cantly the viability of PC12 cells. This was accompanied by an increase in the intracellular calcium levels and cleaved caspase-3. In addition, Aβ induced an increase in Bax, and a decrease in Bcl-2 compared to the controls. However, a pre-treatment with chlorogenic acid rescued the PC12 cells from Aβ by attenuating the elevated intracellular calcium levels and reducing the levels of the apoptosis related proteins, including caspase-3, Bcl-2 and Bax. These results suggest that the protective effects of chlorogenic acid are, at least in parts, by attenuating the intracellular calcium infl ux and reducing apoptosis induced by Aβ. Abstractthat is distributed widely in plants, fruits and vegetables, such as coffee beans, potatoes and apples (Clifford, 1999;Zang et al., 2003). It possesses a wide range of biological activities including anti-carcinogenic, anti-bacterial, anti-infl ammatory and antioxidant activities in vitro (Huang et al., 1988;Almeida et al., 2006;dos Santos et al., 2006). However, the possible benefi cial effects of chlorogenic acid against Aβ have not been elucidated. Therefore, this study evaluated the neuroprotective effects of chlorogenic acid against Aβ-induced toxicity in PC12 cells (rat pheochromocytoma). To accomplish this, an MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay was carried out to determine if chlorogenic acid protected the PC12 cells against Aβ. To examine their underlying mechanisms, the effects of chlorogenic acid on the intracellular calcium level and apoptosis related proteins including Bcl-2, Bax and caspase-3 were evaluated as possible neuroprotective mechanisms against Aβ.

show abstract

The Spice Sage and Its Active Ingredient Rosmarinic Acid Protect PC12 Cells from Amyloid-β Peptide-Induced Neurotoxicity

Cited by 222 publications

References 39 publications

Effects of rosmarinic acid on cognitive and biochemical alterations in ovariectomized rats treated with D-galactose

Effects of rosmarinic acid on cognitive and biochemical alterations in ovariectomized rats treated with D-galactose

Effects of Salvia miltiorrhiza on CNS Neuronal Injury and Degeneration: A Plausible Complementary Role of Tanshinones and Depsides

Protective Effect of Chlorogenic Acid against Aβ-Induced Neurotoxicity

Contact Info

Product

Resources

About