The SPOM-adapted IVM system improves in vitro production of bovine embryos

Leal, Gabriela Ramos; Graciosa, Maria Alice Guimarães; Monteiro, C. A. S.; Pasolini, Renata; Camargo, Agostinho Jorge dos Reis; Oliveira, C. S.; Vasconcelos, Carlos Otávio de Paula; Nogueira, Luiz Altamiro Garcia; Ferreira, Ana Maria Reis; Serapião, R. V.

doi:10.1016/j.theriogenology.2020.09.024

Cited by 9 publications

(3 citation statements)

References 39 publications

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“…More recently, another strategy to increase oocyte and embryo developmental competence has been the control of nuclear maturation by the use of a simulated physiological oocyte maturation (SPOM) system (Rose et al., 2013 ). The latter consists of using cAMP modulators to simulate the maturation mechanisms and enhance synchrony between nuclear and cytoplasmic maturation (Leal et al., 2020 ).…”

Section: In Vitro Embryo Productionmentioning

confidence: 99%

Embryo biotechnologies in sheep: Achievements and new improvements

Falchi

Ledda

Zedda

2022

Reprod Domestic Animals

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Assisted reproductive technologies (ARTs) play a critical role in increasing the dissemination of superior genotypes, reducing generation interval and enhancing productivity. In small ruminants, ARTs encounter limited commercial application compared with the bovine species, although the interest towards these techniques is constantly increasing. According to the International Embryo Transfer Society (IETS) Data Retrieval Report, the total sheep embryo production both in vivo derived (IVD) and in vitro produced

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Section: In Vitro Embryo Productionmentioning

confidence: 99%

Embryo biotechnologies in sheep: Achievements and new improvements

Falchi

Ledda

Zedda

2022

Reprod Domestic Animals

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“…In order to increase embryo cryotolerance and be suitable for DT, important advances have been made in the bovine in vitro production system, improving overall performance of the different stages of the process through multiple strategies. These include improvements in oocyte maturation (Gilchrist et al, 2015; Leal et al, 2020; Luciano & Sirard, 2017), fertilization conditions (Xu et al, 2009), culture media (Chronopoulou & Harper, 2015; Gervasi et al, 2020) and the freezability of embryos (Gómez et al, 2020). It is necessary to understand that the OPU‐IVP system requires a comprehensive overview in order to achieve the greatest impact or sufficiently effective to be incorporated into the commercial IVP laboratory SOP.…”

Section: Impact Of Follicular Wave Manipulation and Differentiationmentioning

confidence: 99%

Transvaginal ultrasound‐guided oocyte retrieval in cattle: State‐of‐the‐art and its impact on the in vitro fertilization embryo production outcome

Ferré

Álvarez-Gallardo

Romo

et al. 2022

Reprod Domestic Animals

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Like any other commercial activity, livestock and seedstock producers pursue regular industry objectives: maximize efficiency and productivity, create value and keep the company profitable. To reach these objectives, various reproductive technology tools have been developed for cattle. Artificial insemination (AI) and embryo technologies (in vivo and/or in vitro) contribute to breeding goals and increase farm productivity through the improvement of specific traits by guided mating (Lamb et al., 2016;Moore & Thatcher, 2006). Embryo technologies (superovulation and embryo collection-MOET or in vivo) and, more recently, in vitro embryo production (IVP), are mainly used to multiply high genetic merit females (Ferré et al., 2019;Galli et al., 2003). The idea/goal is to generate bulls and/or bull dams. In commercial farm operations, embryo technologies are also being applied by either implanting sexed embryos

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“…In vitro maturation (IVM) is probably the most critical part of the whole process of in vitro embryo production (Ferré et al, 2020;Leal et al, 2020). Ruminant oocytes are usually matured at 39°C under 5% CO2 in a humidified atmosphere.…”

Section: Introductionmentioning

confidence: 99%

Effect of two Different Permitting and Combination of Cryoprotectants on Cattle Oocytes Maturation Rate Following Brilliant Cresyl Blue Exposure

Mphaphathi¹,

O'Neill²,

Nedambale³

2022

American Journal of Animal and Veterinary Sciences

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The objectives of this study were to elucidate the toxicity of Dimethyl sulfoxide (DMSO), Ethylene glycol (EG), and the combination of both permitting cryoprotectants (CPAs) to cattle oocytes and the effectiveness of Brilliant cresyl blue (BCB) on immature oocytes pre-selection. Cattle ovaries were collected from the local slaughterhouse and transported within 2 hours of slaughter. The oocytes were exposed to 26 mM BCB solution. The other oocytes were not exposed to BCB solution or CPA. Oocytes were classified as BCB positive (+) or BCB negative (−). Oocytes were either exposed to DMSO, EG, or DMSO + EG at different CPA concentrations as follows: toxicity test 1 (TT1) was 0, 5, 10, 15, and 20%, followed by exposure to TT2 as follows: 10, 20, 30 and 40%. The oocytes were then in vitro matured as per treatment groups. After maturation, oocyte polar body extrusion was evaluated. Treatment means were compared using the Fisher-protected t-test least significant difference. The DMSO treatment group: The oocytes with polar body extrusion were 55.1% (positive control, no BCB and no CPA exposure), 55.0% (control, CPA exposure no BCB), 22.7% [BCB− with CPA toxicity test (DMSO 5 + 10%)], 21.8% [BCB− with CPA toxicity test (DMSO 10 + 20%)] and 7.3% [BCB− with CPA toxicity test (DMSO 20 + 40%)]. The BCB + groups (DMSO 5 + 10 and DMSO 10 + 20%) had more oocytes with polar body extrusion (67.0 and 66.9%, respectively; P>0.05). The EG treatment group: There was a drastic decline of oocytes with polar body extrusion as EG CPA concentration increased (P<0.05). The BCB+ groups (EG 5 + 10 and EG 10 + 20%) had significantly more oocytes with polar body extrusion (68.9 and 51.9%). The DMSO + EG treatment group: There was a decline in oocytes polar body extrusion recorded, as the DMSO + EG CPAs concentrations were increased (P<0.05). The BCB+ groups (DMSO 5 + EG 5% and DMSO 10 + EG 10%) recorded numerically highest number of oocytes with polar body extrusion (55.1 and 55.8%) as compared to positive control (51.1%), respectively (P>0.05). In conclusion, a decline in oocyte polar body extrusion was recorded, as the DMSO, EG, and DMSO + EG concentrations were increased. The BCB can be used to identify developmentally competent oocytes.

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The SPOM-adapted IVM system improves in vitro production of bovine embryos

Cited by 9 publications

References 39 publications

Embryo biotechnologies in sheep: Achievements and new improvements

Embryo biotechnologies in sheep: Achievements and new improvements

Transvaginal ultrasound‐guided oocyte retrieval in cattle: State‐of‐the‐art and its impact on the in vitro fertilization embryo production outcome

Effect of two Different Permitting and Combination of Cryoprotectants on Cattle Oocytes Maturation Rate Following Brilliant Cresyl Blue Exposure

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