The stability and persistence of blood and semen mRNA and miRNA targets for body fluid identification in environmentally challenged and laundered samples

Mayes, Carrie; Houston, Rachel; Seashols‐Williams, Sarah; LaRue, Bobby; Hughes‐Stamm, Sheree

doi:10.1016/j.legalmed.2019.03.007

Cited by 44 publications

(22 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the body fluids often examined in relation to sexual crimes, the most active research is being conducted into the development of methods using gene-targeted mRNA, microRNA, or DNA methylation. Many researchers have been working on the practical application of mRNA methods for several decades, but the problem of mRNA stability remains [96,97]. Currently, microRNAs must be evaluated by the expression patterns of several markers [48][49][50], whereas for DNA methylation, a relatively large amount of template DNA is required [98].…”

Section: Discussionmentioning

confidence: 99%

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

2020

View full text Add to dashboard Cite

Although, DNA typing plays a decisive role in the identification of persons from blood and body fluid stains in criminal investigations, clarifying the origin of extracted DNA has also been considered an essential task in proving a criminal act. This review introduces the importance of developing precise methods for body fluid identification. Body fluid identification has long relied on enzymatic methods as a presumptive assay and histological or serological methods as a confirmatory assay. However, because the latest DNA typing methods can rapidly obtain results from very small and even old, poorly preserved samples, the development of a novel corresponding body fluid identification method is required. In particular, an immunochromatographic method has been introduced to identify saliva and semen from sexual crimes. In addition, for vaginal fluid identification, attempts have been made in the past decade to introduce a method relying on body fluid-specific mRNA expression levels. At present, the development of molecular biological methods involving microRNA, DNA methylation, and resident bacterial DNA is ongoing. Therefore, in criminal investigations, body fluid identification is an essential task for correctly applying the results of DNA typing, although further research and development are required.

show abstract

Section: Discussionmentioning

confidence: 99%

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

2020

View full text Add to dashboard Cite

show abstract

“…Circulating miRNAs are promising biomarkers, highly stable at extreme conditions of pH, chemical treatments and temperature. Moreover, circulating miRNAs are preserved from RNAse activity because they are carried by RNA-binding proteins in extracellular vesicles and lipoprotein [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

MicroRNAs in Hypertrophic, Arrhythmogenic and Dilated Cardiomyopathy

et al. 2021

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are a class of non-coding RNAs of about 20 nucleotides in length, involved in the regulation of many biochemical pathways in the human body. The level of miRNAs in tissues and circulation can be deregulated because of altered pathophysiological mechanisms; thus, they can be employed as biomarkers for different pathological conditions, such as cardiac diseases. This review summarizes published findings of these molecular biomarkers in the three most common structural cardiomyopathies: human dilated, arrhythmogenic and hypertrophic cardiomyopathy.

show abstract

“…Several recent works reported the recovery of some miRNAs after compromising treatments and challenging environmental conditions [ 16 , 17 , 84 ].…”

Section: Mirnas and Time Of Death Determinationmentioning

confidence: 99%

“…let-7 g, let-7i, miR-200b-5p, and miR-891a-5p resisted to UV treatment in blood, urine, semen, and saliva [ 16 ], while miR-451a and let-7g were recovered in blood and semen stains aged six months exposed to different experimental conditions: sunlight, humidity (99%) and heat (60%) [ 17 ]. These miRNAs persisted in laundered stains [ 17 ]. Minimal degradation of others miRNAs (including miR-451a) in bloodstains aged five months at 37 °C was observed also by Fang et al [ 84 ].…”

Section: Mirnas and Time Of Death Determinationmentioning

confidence: 99%

“…Most miRNAs are located within the cell, but some of them have also been found in various body fluids including plasma, tears, cerebrospinal fluid, and saliva, and are commonly being referred to as circulating miRNAs [14,15]. These circulating miRNAs are a particularly promising class of biomarkers given several characteristics they share: first, unlike other RNA classes, they are highly stable even at extreme conditions of temperature, pH, and chemical treatments; and they are preserved from endogenous RNase-activity as they are carried by lipoprotein complexes and RNA-binding proteins in extracellular vesicles [16,17]. miRNA analysis can be performed with profiling methods such as microarray or next generation sequencing (NGS) techniques that allow simultaneous detection of even hundreds of low copy number miRNAs in a single experiment [18].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

MicroRNAs: An Update of Applications in Forensic Science

et al. 2020

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are a class of non-coding RNAs containing 18–24 nucleotides that are involved in the regulation of many biochemical mechanisms in the human body. The level of miRNAs in body fluids and tissues increases because of altered pathophysiological mechanisms, thus they are employed as biomarkers for various diseases and conditions. In recent years, miRNAs obtained a great interest in many fields of forensic medicine given their stability and specificity. Several specific miRNAs have been studied in body fluid identification, in wound vitality in time of death determination, in drowning, in the anti-doping field, and other forensic fields. However, the major problems are (1) lack of universal protocols for diagnostic expression testing and (2) low reproducibility of independent studies. This review is an update on the application of these molecular markers in forensic biology.

show abstract

The stability and persistence of blood and semen mRNA and miRNA targets for body fluid identification in environmentally challenged and laundered samples

Abstract: The stability and persistence of blood and semen mRNA and miRNA targets for body fluid identification in environmentally challenged and laundered samples, Legal Medicine (2019), doi:

Cited by 44 publications

References 46 publications

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

MicroRNAs in Hypertrophic, Arrhythmogenic and Dilated Cardiomyopathy

MicroRNAs: An Update of Applications in Forensic Science

Contact Info

Product

Resources

About