The stability of 99mTc-MIBI (Sestamibi) complex samples which prepared under ultrasound irradiation technique versus boiling water bath method

Doroudi, Alireza; Saraji, Farkondeh; Erfani, Mostafa; Saadati, Seyyed Mostafa; Kisast, Ali; Ahmadi, Fereshte; Etesami, Behrooz

doi:10.7324/japs.2016.601120

Cited by 5 publications

(5 citation statements)

References 21 publications

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“…99m Tc-labeled hexakis-methoxyisobutylisonitrile ( 99m Tc-MIBI) is a lipophilic complex with a positive charge and is a well-known myocardial perfusion SPECT agent in the clinical field [40,41]. In the 99m Tc-MIBI complex, 99m Tc(I) is a soft metal and is complexed by six soft isonitrile ligands in a hexavalent fashion to form a stable octahedral complex [42]. The metal center of [ 99m Tc][Tc(CO) 3 (H 2 O) 3 ] + is likewise soft and the isonitrile ligands can strongly coordinate with it in a 3:1 ratio, as reported previously [43][44][45].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Evaluation of 99mTc-Tricabonyl Labeled Isonitrile Conjugates for Prostate-Specific Membrane Antigen (PSMA) Image

et al. 2020

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Prostate-specific membrane antigen (PSMA) is a biomarker expressed on the surface of prostate cancer (PCa). In an effort to improve the detection and treatment of PCa, small urea-based PSMA inhibitors have been studied extensively. In the present study, we aimed to develop 99mTc-tricabonyl labeled urea-based PSMA conjugates containing isonitrile (CN-R)-coordinating ligands ([99mTc]Tc-15 and [99mTc]Tc-16). Both the PSMA conjugates were obtained at high radiochemical efficiency (≥98.5%). High in vitro binding affinity was observed for [99mTc]Tc-15 and [99mTc]Tc-16 (Kd = 5.5 and 0.2 nM, respectively) in PSMA-expressing 22Rv1 cells. Tumor xenografts were conducted using 22Rv1 cells and rapid accumulation of [99mTc]Tc-16 (1.87 ± 0.11% ID/g) was observed at 1 h post-injection, which subsequently increased to (2.83 ± 0.26% ID/g) at 4 h post-injection. However, [99mTc]Tc-15 showed moderate tumor uptake (1.48 ± 0.18% ID/g), which decreased at 4 h post-injection (0.81 ± 0.09% ID/g). [99mTc]Tc-16 was excreted from non-targeted tissues with high tumor-to-blood (17:1) and tumor-to-muscle ratio (41:1) at 4 h post-injection at approximately 4 times higher levels than [99mTc]Tc-15. Uptakes of [99mTc]Tc-15 and [99mTc]Tc-16 to PSMA-expressing tumor and tissues were significantly blocked by co-injection of 2-(Phosphonomethyl)-pentandioic acid (2-PMPA), suggesting that their uptakes are mediated by PSMA specifically. Whole-body single photon emission computed tomography imaging of [99mTc]Tc-16 verified the ex vivo biodistribution results and demonstrated clear visualization of tumors and tissues expressing PSMA compared to [99mTc]Tc-15. In conclusion, using [99mTc]Tc-16 rather than [99mTc]Tc-15 may be the preferable because of its relatively high tumor uptake and retention.

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Section: Introductionmentioning

confidence: 99%

Synthesis and Evaluation of 99mTc-Tricabonyl Labeled Isonitrile Conjugates for Prostate-Specific Membrane Antigen (PSMA) Image

et al. 2020

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“…Ultrasound waves have chemical effects on the reaction system due to the creation of cavitation or generation of free radicals which augment the rate of reaction. Green chemistry has been recommended for the preparation of technetium-99m methoxy isobutyl isocyanate ( 99m Tc-MIBI) radiotracer samples in nuclear medicine [17][18][19] . The principal aim of this investigation was to evaluate the efficacy of ultrasound irradiation technique to reconstitute the freeze-dried Octreotidekits with 99m Tc radionuclide in comparison to the boiling water bath standard method.…”

Section: Introductionmentioning

confidence: 99%

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2019

IJPSR

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This approach was launched to evaluate the application of ultrasound waves for the preparation of 99m Tc-Octreotide radio conjugate sample. The 740MBq (20 mCi) of 99m Tc-Octreotide samples were prepared by the boiling water bath (standard) or ultrasound irradiation methods. The stability of samples in normal saline and human serum albumin was investigated. The partition coefficient and protein bonding of samples were determined. The radioisotope analysis was undertaken to assess the biodistribution of radiotracer samples in rats. The data obtained from this approach revealed that the 99m Tc-Octreotide samples were successfully prepared by the newly developed technique. Statistically significant differences have not been observed in the stability, partition coefficient and protein bonding of radiotracer samples which were prepared by either of the two above-mentioned. The radioisotope analysis showed that the applied method for radiolabeling did not lead to a significant difference in the biodistribution pattern of radiotracer samples in rats. Green chemistry can be recommended for the radiolabeling of Octreotide with 99m Tc as an alternative and reliable modality for the boiling water bath method.

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“…In order to carry out the radiolabeling reaction of the ligand with 99m Tc radioisotope, the ligand structure must contain functional groups that contain non-bonded electrons such as oxygen, nitrogen, sulfur, phosphorus etc, to react with empty orbitals of reduced Tc is readily performed at room temperature for radiolabeling reaction 22 . However, in some ligands, the radiolabeling reaction is facilitated by heating due to the fact the functional groups in these ligand structure do not share non-bonding electrons readily with the reduced 99m Tc [19][20][21]23,24 . Boiling water bath device is routinely used for heating source in nuclear medicine centers.…”

Section: Mmentioning

confidence: 99%

“…The reactions under ultrasound waves can be leaded to better yields, faster rates and milder temperatures [15][16][17][18] . The new developed technique was examined to prepare the 99m TcSestamibi radiocomplex as an alternative and reliable method in clinical practice [19][20][21] . This approach was conducted to evaluate the application of ultrasound irradiation for the reconstitution MAG 3 freeze-dried kits by 99m Tc radioisotope in comparison to the boiling water bath as a standard method.…”

Section: Introductionmentioning

confidence: 99%

The Preparation of 99mTc-MAG3 radiotracer by Ultrasound

Doroudi¹,

Falavarjani²,

Khodayar³

et al. 2018

IJPTR

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:The main aim of this investigation was to prepare 99m Tc-MAG 3 samples under sonication. Then the radio complex samples were compared with the counterparts which prepared by boiling water bath as the standard method from the aspects of stability in normal saline, human serum, partition coefficient, protein bonding and finally the biodistribution in rat.The preparation of 99m Tc-MAG 3 samples was examined to determine the ideal condition under sonication that radio complex samples could be prepared with appropriate radiochemical purity. The stability of radiotracer samples was assessed in normal saline up to 24 h post preparation. The partition coefficient, protein bonding and stability in human serum were analyzed. Then the biodistribution of radiotracer samples were evaluated in rat.The Radio-HPLC and ITLC assays indicated that the 99m Tc-MAG 3 samples could be successfully prepared with suitable yields by sonication. The radiolabeling efficiency was above the 90% when the reaction was carried out at 60°C for 1 min. The radio complex samples showed good stability in normal saline and human serum. The partition coefficient and protein bonding were -2.4± 0.32 and 35.23± 1.15 respectively when the radiolabeling was performed by the standard method. These values were -2.51± 0.42 and 39.36± 1.7 when the radiolabeling was undertaken by sonication. The biodistribution of 99m Tc-MAG 3 samples in the rats demonstrated that the radiolabeling procedure could not lead to a significant difference in the biodistribution of samples. The new developed technique can be recommended as an alternative for boiling water bath method to prepare 99m Tc-MAG 3 .

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The stability of 99mTc-MIBI (Sestamibi) complex samples which prepared under ultrasound irradiation technique versus boiling water bath method

Cited by 5 publications

References 21 publications

Synthesis and Evaluation of 99mTc-Tricabonyl Labeled Isonitrile Conjugates for Prostate-Specific Membrane Antigen (PSMA) Image

Synthesis and Evaluation of 99mTc-Tricabonyl Labeled Isonitrile Conjugates for Prostate-Specific Membrane Antigen (PSMA) Image

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The Preparation of 99mTc-MAG3 radiotracer by Ultrasound

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