The starch-sugar interconversion mechanism during bulb development of Cardiocrinum giganteum (Wall.) Makino revealed by transcriptome and metabolite analysis

Zhao, Zhiheng; Li, Rongchen; Wang, Xiaojing; Liang, Wenhui; Liao, Jianming; Huang, Xiaolu; Cai, Zian; Liú, Dan; Huang, Lan; Wei, Xuefang; Guang-yu, Zeng; Chen, Yuzhen; Chen, Chongzheng; Lu, Cunfu

doi:10.1016/j.indcrop.2022.115318

Cited by 8 publications

(6 citation statements)

References 42 publications

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“…Starch and sucrose regulatory pathway is crucial in maintaining the dynamic balance of sugar substances and energy homeostasis in plant growth and development by regulating the interconversion of starch and sugar (Zhao et al, 2022). Some studies have suggested that sucrose serves as a substrate for the formation of storage reserves and as a main nutrient and energy source for plant development (Yu et al, 2016;Ma et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…ZmAGPs and ZmSSs were upregulated at stage II and the activity of AGPase increased, which enhanced the carbon flux into starch. b-glucosidase (bglX) and amylase are the key enzymes involved in the process of starch decomposition, affecting seed germination and seedling growth (Zhao et al, 2022). a-amylase is a major carbohydrase that catalyzes the hydrolysis of a-(1, 4) glycosidic linkages in starch, glycogen, and related polysaccharides (Huang et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

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Insights into the regulation of energy metabolism during the seed-to-seedling transition in marine angiosperm Zostera marina L.: Integrated metabolomic and transcriptomic analysis

Zhu

Zang²,

Zhang³

et al. 2023

Front. Plant Sci.

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Seed development is a crucial phase in the life cycle of seed-propagated plants. As the only group of angiosperms that evolved from terrestrial plants to complete their life cycle submerged in marine environments, the mechanisms underlying seed development in seagrasses are still largely unknown. In the present study, we attempted to combine transcriptomic, metabolomic, and physiological data to comprehensively analyze the molecular mechanism that regulates energy metabolism in Zostera marina seeds at the four major developmental stages. Our results demonstrated that seed metabolism was reprogrammed with significant alteration of starch and sucrose metabolism, glycolysis, the tricarboxylic acid cycle (TCA cycle), and the pentose phosphate pathway during the transition from seed formation to seedling establishment. The interconversion of starch and sugar provided energy storage substances in mature seeds and further acted as energy sources to support seed germination and seedling growth. The glycolysis pathway was active during Z. marina germination and seedling establishment, which provided pyruvate for TCA cycle by decomposing soluble sugar. Notably, the biological processes of glycolysis were severely inhibited during Z. marina seed maturation may have a positive effect on seed germination, maintaining a low level of metabolic activity during seed maturation to preserve seed viability. Increased acetyl-CoA and ATP contents were accompanied with the higher TCA cycle activity during seed germination and seedling establishment, indicating that the accumulations of precursor and intermediates metabolite that can strengthen the TCA cycle and facilitate energy supply for Z. marina seed germination and seedling growth. The large amount of oxidatively generated sugar phosphate promotes fructose 1,6-bisphosphate synthesis to feed back to glycolysis during seed germination, indicating that the pentose phosphate pathway not only provides energy for germination, but also complements the glycolytic pathway. Collectively, our findings suggest these energy metabolism pathways cooperate with each other in the process of seed transformation from maturity to seedling establishment, transforming seed from storage tissue to highly active metabolic tissue to meet the energy requirement seed development. These findings provide insights into the roles of the energy metabolism pathway in the complete developmental process of Z. marina seeds from different perspectives, which could facilitate habitat restoration of Z. marina meadows via seeds.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Insights into the regulation of energy metabolism during the seed-to-seedling transition in marine angiosperm Zostera marina L.: Integrated metabolomic and transcriptomic analysis

Zhu

Zang²,

Zhang³

et al. 2023

Front. Plant Sci.

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“…Starch and sucrose metabolism comprised the main enrichment metabolic pathway, and there was an increase in the abundance of glucose, fructose, sucrose, fructose-6-phosphate, glucose-6-phosphate, and other saccharides in the high-yield tobacco samples from the two regions. The metabolic pathways of starch and sucrose metabolism are complex biochemical processes that rely on the synergistic action of multiple enzymes [40]. Phenylpropane metabolism intermediates are crucial for plant growth and the long-distance transport of water and nutrients while also aiding plant defence against abiotic and biotic stresses [41,42].…”

Section: Characteristic Metabolites and Metabolic Pathways Of Differe...mentioning

confidence: 99%

Exploring Metabolic Characteristics in Different Geographical Locations and Yields of Nicotiana tabacum L. Using Gas Chromatography–Mass Spectrometry Pseudotargeted Metabolomics Combined with Chemometrics

Jing,

Chen,

Qiu

et al. 2024

Metabolites

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The quality of crops is closely associated with their geographical location and yield, which is reflected in the composition of their metabolites. Hence, we employed GC–MS pseudotargeted metabolomics to investigate the metabolic characteristics of high-, medium-, and low-yield Nicotiana tabacum (tobacco) leaves from the Bozhou (sweet honey flavour) and Shuicheng (light flavour) regions of Guizhou Province. A total of 124 metabolites were identified and classified into 22 chemical categories. Principal component analysis revealed that the geographical location exerted a greater influence on the metabolic profiling than the yield. Light-flavoured tobacco exhibited increased levels of sugar metabolism- and glycolysis-related intermediate products (trehalose, glucose-6-phosphate, and fructose-6-phosphate) and a few amino acids (proline and leucine), while sweet honey-flavoured tobacco exhibited increases in the tricarboxylic acid cycle (TCA cycle) and the phenylpropane metabolic pathway (p-hydroxybenzoic acid, caffeic acid, and maleic acid). Additionally, metabolite pathway enrichment analysis conducted at different yields and showed that both Shuicheng and Bozhou exhibited changes in six pathways and four of them were the same, mainly C/N metabolism. Metabolic pathway analysis revealed higher levels of intermediates related to glycolysis and sugar, amino acid, and alkaloid metabolism in the high-yield samples, while higher levels of phenylpropane in the low-yield samples. This study demonstrated that GC–MS pseudotargeted metabolomics-based metabolic profiling can be used to effectively discriminate tobacco leaves from different geographical locations and yields, thus facilitating a better understanding of the relationship between metabolites, yield, and geographical location. Consequently, metabolic profiles can serve as valuable indicators for characterizing tobacco yield and geographical location.

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“…In addition, research on C . giganteum has focused mainly on bulb development [ 37 ], seed dormancy [ 38 ], and phylogenetic analysis [ 1 , 39 ]. Pollen development during the process of inflorescence differentiation in this species has been studied, but the morphological and molecular mechanisms related to other developmental stages have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Time-Course Transcriptomic Analysis Reveals Molecular Insights into the Inflorescence and Flower Development of Cardiocrinum giganteum

Wei,

Li,

Zhao

et al. 2024

Plants

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Cardiocrinum giganteum is an endemic species of east Asia which is famous for its showy inflorescence and medicinal bulbs. Its inflorescence is a determinate raceme and the flowers bloom synchronously. Morphological observation and time-course transcriptomic analysis were combined to study the process of inflorescence and flower development of C. giganteum. The results show that the autonomic pathway, GA pathway, and the vernalization pathway are involved in the flower formation pathway of C. giganteum. A varied ABCDE flowering model was deduced from the main development process. Moreover, it was found that the flowers in different parts of the raceme in C. giganteum gradually synchronized during development, which is highly important for both evolution and ecology. The results obtained in this work improve our understanding of the process and mechanism of inflorescence and flower development and could be useful for the flowering period regulation and breeding of C. giganteum.

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The starch-sugar interconversion mechanism during bulb development of Cardiocrinum giganteum (Wall.) Makino revealed by transcriptome and metabolite analysis

Cited by 8 publications

References 42 publications

Insights into the regulation of energy metabolism during the seed-to-seedling transition in marine angiosperm Zostera marina L.: Integrated metabolomic and transcriptomic analysis

Insights into the regulation of energy metabolism during the seed-to-seedling transition in marine angiosperm Zostera marina L.: Integrated metabolomic and transcriptomic analysis

Exploring Metabolic Characteristics in Different Geographical Locations and Yields of Nicotiana tabacum L. Using Gas Chromatography–Mass Spectrometry Pseudotargeted Metabolomics Combined with Chemometrics

Time-Course Transcriptomic Analysis Reveals Molecular Insights into the Inflorescence and Flower Development of Cardiocrinum giganteum

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