The stingless bee honey protects against hydrogen peroxide-induced oxidative damage and lipopolysaccharide-induced inflammation in vitro

Abstract

Oxidative stress, DNA damage, and unresolved inflammation are the predisposing factors of many chronic and degenerative diseases, including cancer. Stingless bee honey (SBH) is recognized to have high medicinal value by traditional medicine practitioners and has been used to treat various illnesses traditionally. This study aimed to determine the antioxidant, anti-inflammatory, and genoprotective effects of SBH by using in vitro cell culture models. The sugar content, total phenolic content, radical scavenging activity, and ferric reducing antioxidant power (FRAP) of SBH were determined in this study. Then, the protective effect of SBH against hydrogen peroxide (H 2 O 2 )-induced cell death and DNA damage was studied by using WIL2-NS human lymphoblastoid cell line, while the lipopolysaccharide (LPS)-induced RAW 264.7 murine macrophages cell line was used to study the anti-inflammatory effects of SBH. Results from this present study showed that the major sugar contents of SBH were fructose (19.39 + 0.01%) and glucose (14.03 ± 0.03%). Besides, the total phenolic content, the radical scavenging activity, and the FRAP value of SBH were 15.38 ± 0.02 mg GAE/100 g of honey, 34.04 ± 0.21%, and 206.77 + 1.76 μM AAE/100 g honey respectively. Pretreatment with SBH protected WIL2-NS cells from H 2 O 2 -induced cell death and DNA damage (p < 0.001). Moreover, SBH was also able to attenuate the production of nitric oxide by inhibiting the expression of inducible nitric oxide synthase in LPS-induced RAW 264.7 cells (p < 0.001). In conclusion, SBH is rich in total phenolic content and possesses strong antioxidant, anti-inflammatory, and genoprotective properties. Our current findings suggest that SBH might be useful in the prevention and treatment of many diseases caused by oxidative stress and inflammation assuming the observed effects are also achievable in vivo.

A Review on Recent Progress of Stingless Bee Honey and Its Hydrogel-Based Compound for Wound Care Management

“…It can produce soreness, edema, and exudation, and it can slow or restrict the healing process [ 77 ]. Under normal circumstances, the inflammatory response removes infectious organisms from the body and aids in tissue repair [ 78 ]. However, prolonged inflammation results in a progressive shift in the cell type present at the inflammatory site and is characterized by the simultaneous destruction and repair of the inflammatory tissue [ 78 , 79 , 80 ].…”

“…Under normal circumstances, the inflammatory response removes infectious organisms from the body and aids in tissue repair [ 78 ]. However, prolonged inflammation results in a progressive shift in the cell type present at the inflammatory site and is characterized by the simultaneous destruction and repair of the inflammatory tissue [ 78 , 79 , 80 ]. As a result, pathological diseases such as autoimmune disease, tissue fibrosis, or tumor growth may develop [ 78 ].…”

“…However, prolonged inflammation results in a progressive shift in the cell type present at the inflammatory site and is characterized by the simultaneous destruction and repair of the inflammatory tissue [ 78 , 79 , 80 ]. As a result, pathological diseases such as autoimmune disease, tissue fibrosis, or tumor growth may develop [ 78 ]. Numerous anti-inflammatory medications have been produced; however, they are incompatible with wound healing.…”

Stingless bees honeys': physical-chemical characterization, difficulties and challenges

“…When compared to honey from Apis mellifera, the honey produced by stingless bees has several sensory particularities, like less sweetness, more acidic flavor and more fluid texture, as well as some different physicochemical properties, especially in sugars composition and amount, moisture and acidity (Nordin et al, 2018). Studies show that honey from stingless bees, in addition to having different nutritional properties, has therapeutic applications with promising results, with the presence of antioxidant capacity bioactive compounds (Ávila et al, 2018a;Biluca et al, 2016;Ooi et al, 2021), potential to antimicrobial (Khongkwanmueang et al, 2020) and antiproliferative cell activities (Ismail et al, 2018).…”

“…The diversity of geographic regions, soil characteristics, climate, and vegetation, in addition to the enormous the variety of stingless bee species impacts directly in the characteristics of the honey produced (Nordin et al, 2018) and studies of these different factors are essential for the development of specific laws and norms for stingless bee honey (Biluca et al, 2016;Marcolin et al, 2021). Meliponiculture is an activity with great growth potential and the management of improvements in beekeeping practices reflect the significant expansion of the market for stingless bees' products, with increasing numbers of consumers (Jaffé et al, 2015), in addition to already being recognized for its high medicinal content (Al-Hatamleh et al, 2020;Ooi et al, 2021).…”