The structural organization of mouse metaphase chromosomes

Schreck, R.R.; Dev, V.G.; Erlanger, Bernard F.; Miller, Orlando J.

doi:10.1007/bf00327032

Cited by 19 publications

(3 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In summary, our data seem to confirm (1) that photo-oxidation acts by preferential destruction of guanine residues and (2) that the DNA denaturation is more effective in GC-rich regions, as Miller et al (1973) and Schreck et al (1973Schreck et al ( , 1977 proposed.…”

Section: Discussionsupporting

confidence: 74%

“…In fact, given the known base composition of satellite DNA's in three of the species we studied, a correspondence seems to exist between molecular hybridization in situ of AT-rich satellite DNA's and the location of specific stain reactions in specific chromosome regions. Moreover, our results support previous findings suggesting preferential guanine destruction as a con sequence of photo-oxidation.Visible light, oxygen, and certain com pounds, such as methylene blue, interact in the presence of both purified and chromo somal DNA, resulting in an alteration of the structural organization of DNA (Simon and Van Vunakis, 1962;Miller et al, 1973;Schreck et al, 1973Schreck et al, , 1977Drets et al, 1978: M ezzanotte et al, 1979h\ Bianchi et al., 1980. When photo-oxidation of eukar yote chromosomes is followed by a number of staining reactions, a longitudinal differ entiation becomes evident.…”

mentioning

confidence: 99%

“…et al, , 1977Drets et al, 1978;Mezza-NOTTE et al, 19796: Bianchi et al, 1980). While singlet oxygen, an extremely reac tive species of Os, is thought to be the agent responsible for chemical alteration of the chromosomal structure (Bianchi et al, 1980), guanine destruction is believed to be the primary effect of photo-oxidation in both purified and chromosomal DNA (Simon and Van Vunakis, 1962;Miller et al, 1973;Schreck et al, 1973Schreck et al, , 1977Mezzanotte et al, 19796). Drets et al (1978), however, question the specificity of guanine destruc tion in photo-oxidized fixed chromosomes and propose a preferential destruction and extraction of DNA in bands containing more AT-rich DNA.…”

mentioning

confidence: 99%

See 2 more Smart Citations

The longitudinal differentiation produced by photo-oxidation and acridine-orange staining in eukaryote chromosomes: role and involvement of DNA base composition

Mezzanotte

Ferrucci

Marchi

et al. 1982

Cytogenet Genome Res

View full text Add to dashboard Cite

Photo-oxidation and staining with acridine-orange result in a longitudinal green/orange-red/brown-red color differentiation in fixed chromosomes of Drosophila melanogaster, D. virilis, Culiseta longiareolata, and Mus musculus. Such a result has been correlated with specific DNA base sequences situated in specific chromosomal areas. In fact, given the known base composition of satellite DNA’s in three of the species we studied, a correspondence seems to exist between molecular hybridization in situ of AT-rich satellite DNA’s and the location of specific stain reactions in specific chromosome regions. Moreover, our results support previous findings suggesting preferential guanine destruction as a consequence of photo-oxidation.

show abstract

Section: Discussionsupporting

confidence: 74%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

The longitudinal differentiation produced by photo-oxidation and acridine-orange staining in eukaryote chromosomes: role and involvement of DNA base composition

Mezzanotte

Ferrucci

Marchi

et al. 1982

Cytogenet Genome Res

View full text Add to dashboard Cite

show abstract

Interactions between pairs of DNA‐binding dyes: Results and implications for chromosome analysis

et al. 1980

View full text Add to dashboard Cite

A number of DNA-binding dyes, with spectral properties making them suitable as components of energy donor-acceptor pairs, are described. If such pairs are used to stain metaphase chromosomes, and if the energy acceptor (e.g., actinomycin D or methyl green) has a binding specificity opposite to the binding or fluorescence specificity of the donor (e.g,, 33258 Hoechst, quinacrine or chromomycin A3), contrast in donor fluorescence can be enhanced, leading to patterns selectively highlighting standard or reverse chromosome bands or particular polymorphic regions. Such results presumably reflect chromosomal regions enriched in 10-20 base pair clusters to which the donor binds and fluoresces but to which the acceptor cannot bind. For other pairs, involving counterstains such as netropsin or echinomycin, which are not suitable as energy acceptors, specific changes observed in polymorphic region fluorescence are most likely due to binding competition between dyes. Dye pairs producing contrast by either method can be used to differentiate between homologous chromosomes or to facilitate detection of specific chromosomal rearrangements. Preliminary data indicate that contrast enhancement generated in fixed metaphase chromosomes spread on microscopic slides can also be observed in suspensions of unfixed metaphase chromosomes, reinforcing the expectation that the methodology described will be of use in flow cytometry.Key terms: Energy transfer, fluorescence, binding competition, chromosome banding patterns, polymorphisms, rearrangements, flow cytometry.Staining metaphase chromosomes simultaneously with pairs of different dyes can lead t o new fluorescence patterns. In addition to the signals produced when the dyes are used independently, information can be generated by interactions between these dyes. For example, two dyes can compete for binding to certain chromosomal sites, which can thereby be stained differentially (15,41) Alternatively, indirect effects of one dye on another can be mediated via conformational changes induced in DNA (46). In addition, because of a coincidence between the range of separations possible between ' Supported by Grant GM21121 from the National Institutes of Health. S.A.L. is the recipient of a Research Career Development Award GM00122 from the National Institute of General Medical Sciences and E.S. is supported in part by a grant from the Whitaker Foundation.* Presented in part at Automated Cytology VII, Asilomar, California, November 25-30, 1979. dye molecules bound to DNA (20) and the effective distance over which dye electronic transition dipoles can interact (typically 5 50 A) (9, 42), the transfer of electronic excitation energy from one dye to another in doubly stained chromatin can be appreciable (14,16,17,36).We have recently shown that the enhanced contrast in metaphase chromosome staining produced by certain dye pairs is due largely to energy transfer, while that produced by other pairs, which fail to satisfy spectral overlap criteria, is due primarily to binding competition (37)....

show abstract

Immunological Detection of Radiation Damage in DNA

Vunakis

1980

Photochemical and Photobiological Reviews

View full text Add to dashboard Cite

The structural organization of mouse metaphase chromosomes

Cited by 19 publications

References 37 publications

The longitudinal differentiation produced by photo-oxidation and acridine-orange staining in eukaryote chromosomes: role and involvement of DNA base composition

The longitudinal differentiation produced by photo-oxidation and acridine-orange staining in eukaryote chromosomes: role and involvement of DNA base composition

Interactions between pairs of DNA‐binding dyes: Results and implications for chromosome analysis

Immunological Detection of Radiation Damage in DNA

Contact Info

Product

Resources

About