The Study of Bacillus Subtils Antimicrobial Activity on Some of the Pathological Isolates

Biofilm inhibition has been identified as a novel drug target for the development of broad-spectrum antibiotics to combat infections caused by drug-resistant bacteria. Although several plant-based compounds have been reported to have anti-biofilm properties, research on the anti-biofilm properties of bacterial bioactive compounds has been sparse. In this study, the efficacy of compounds from a cell-free supernatant of Bacillus subtilis against a biofilm formation of Pseudomonas sp. was studied through in vitro, in vivo and in silico studies. Here, in well diffusion method, Bacillus subtilis demonstrated antibacterial activity, and more than 50% biofilm inhibition activity against Pseudomonas sp. was exhibited through in vitro studies. Moreover, molecular docking and molecular dynamics (MD) simulation gave insights into the possible mode of action of the bacterial volatile compounds identified through GC-MS to inhibit the biofilm-formation protein (PDB ID: 7M1M) of Pseudomonas sp. The binding energy revealed from docking studies ranged from −2.3 to −7.0 kcal mol−1. Moreover, 1-(9H-Fluoren-2-yl)-2-(1-phenyl-1H-ttetrazole5-ylsulfanyl)-ethanone was found to be the best-docked compound through ADMET and pharmacokinetic properties. Furthermore, MD simulations further supported the in vitro studies and formed a stable complex with the tested protein. Thus, this study gives an insight into the development of new antibiotics to combat multi-drug-resistant bacteria.

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“…Additionally, it was established through additional research that Bacillus subtilis was effective against Pseudomonas sp. [50,51].…”

Section: Discussionmentioning

confidence: 99%

Cell-Free Supernatants (CFSs) from the Culture of Bacillus subtilis Inhibit Pseudomonas sp. Biofilm Formation

et al. 2022

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“…This mechanism has allowed antibiotic resistance genes to spread quickly among Enterobacteriaceae and other pathogens like Acinetobacter baumanii 13 . Antibiotic resistance genes 14 , such as the extended-spectrumlactamase CTX-M-15 in Escherichia coli ST131 and KPC in Klebsiella pneumoniae sequence type (ST) 258, may clonally propagate in successfully pathogenic strains 15,16 . Due to HGT and clonal growth 17 , KPC and NDM-1 quickly spread after their first appearance.…”

Section: Introductionmentioning

confidence: 99%

Genotypic Detection of Carbapenems Resistance Genes in Acinetobacter baumannii Isolated from Urinary Tract Infection Patients

Hayder Hasan,

Yasir AL-Ethari,

Ghani Al-Muhanna

et al. 2024

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Acinetobacter baumannii is a Gram-negative bacterium characterized by its short, round, rod-shaped morphology. It is an opportunistic pathogen that poses a significant threat, particularly to immunocompromised patients, often those with hospital stays lasting less than 90 days. Between June 2022 and July 2023, 214 urine samples were collected from individuals suspected of having urinary tract infections (UTIs). These samples were subjected to antibiotic resistance testing, focusing on detecting specific genes related to carbapenem resistance, namely blaNDM, blaKPC, and blaVIM.The study's results revealed a notable trend in antibiotic resistance among the bacterial isolates. Ceftazidime, cefotaxime, and ceftriaxone, commonly used antibiotics for UTIs, showed a high resistance rate among the tested isolates. This resistance highlights the challenges healthcare professionals face when treating UTIs caused by Acinetobacter baumannii. On the other hand, the isolates displayed a comparatively lower resistance rate to imipenem and meropenem, two necessary carbapenem antibiotics. This lower resistance to carbapenems is encouraging as these drugs are often considered the last line of defense against multidrug-resistant bacterial infections. The presence of carbapenem resistance genes, such as blaNDM, blaKPC, and blaVIM, in the Acinetobacter baumannii isolates is of particular concern. These genes confer resistance to carbapenem antibiotics, crucial for treating severe infections caused by multidrug-resistant bacteria. In conclusion, the study aims to study the growth of antibiotic resistance in Acinetobacter baumannii, especially in urinary tract infections in immunocompromised patients with more extended hospital stays. It also highlights the need for Surveys and periodic examinations to detect the spread of bacteria and their resistance. Keywords: Carbapenems, UTI, genes, blaNDM, blaKPC, and blaVIM.

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“…Exopolysaccharide synthesis and biofilm development are induced by adhesion to the catheter surface or bladder epithelium (Nicolle, 2014). Sera from rheumatoid patients exhibit higher levels of Proteus mirabilis IgM antibodies than those from other individuals (Hayder and Aljanaby, 2019b;Kadhum and Hasan, 2019). Through a molecular mimicry mechanism, Proteus mirabilis is hypothesized to contribute to the development of RA in susceptible individuals.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Proteus mirabilis proteins by using SDS-page technique in rheumatoid arthritis patients

Kamil

Aldahhan

2022

ijhs

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This study was aimed to detecting difference in proteins separation by SDS-Page for Proteus mirabilis isolated from urine of non-Rheumatoid arthritis patients have urinary tract infection , urine from Rheumatoid arthritis patients have urinary tract infection. Two hundred (200) urine sample collected from non RA patient30 urine sample from RA patient . These clinical samples were collected and cultured on blood agar and macConky agar. 32 isolates were identified as p. mirabilis by using biochemical test and VITEK 2 system used to complete the identification. The current study used SDS-Page technique for proteins analysis of Proteus mirabilis for both RA patient and non RA patient to detect the differences between them and the result appeared that there are no differences in total protein, insoluble proteins and soluble protein at different pH. Values (6.0, 7.0 & 8.0) at 37 cº, also the result shown differences in the cell –free culture supernatants between Proteus mirabilis from urine of both rheumatoid arthritis patients and non-rheumatoid arthritis patient in pH.8.0 at 37 cº in 7 h. by band appeared in pH.8.0 at 37 cº in 7 h in RA .and there is no band in non RA at the same condition.

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The Study of Bacillus Subtils Antimicrobial Activity on Some of the Pathological Isolates

Cited by 14 publications

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Cell-Free Supernatants (CFSs) from the Culture of Bacillus subtilis Inhibit Pseudomonas sp. Biofilm Formation

Cell-Free Supernatants (CFSs) from the Culture of Bacillus subtilis Inhibit Pseudomonas sp. Biofilm Formation

Genotypic Detection of Carbapenems Resistance Genes in Acinetobacter baumannii Isolated from Urinary Tract Infection Patients

Analysis of Proteus mirabilis proteins by using SDS-page technique in rheumatoid arthritis patients

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