The study of structure and photophysical processes of polyelectrolyte-surfactant-dye molecules complex by laser spectroscopy

Bulakov, D. V.; Saletsky, A. M.

doi:10.1002/lapl.200710013

Cited by 7 publications

(6 citation statements)

References 9 publications

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“…1 Also, this quantity is sensitive to many environmental factors such as temperature, pH, viscosity, ionic concentration, oxygen saturation, and influenced by bonds or molecular associations. [2][3][4] Because of these characteristics, fluorescence lifetime imaging microscope (FLIM) provides high contrast in studies of various diseases, including arthritis, atherosclerosis, and cancer when imaging tissue autofluorescence. 5,6 In particular, the major current application of FLIM in the local fluorophore environment is the detection of Föster resonant energy transfer (FRET) between molecules.…”

Section: Introductionmentioning

confidence: 99%

Spectrally resolved fluorescence lifetime imaging microscope using tunable bandpass filters

Jeong

Ahn

et al. 2012

Review of Scientific Instruments

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A simple structure of spectral fluorescence lifetime imaging microscope (SLIM) is designed with the use of tunable bandpass filter, a kind of Fabry-perot filter that transmission wavelength is varying according to incident angle of light. Feasibility tests of this angle-tuned bandpass filter (ATBF) are performed and it shows high transmission and constant spectral bandwidth (20 nm) with respect to angle of incidence. Furthermore, using two ATBFs in series, spectral bandwidth can be adjustable down to 4 nm. In this paper, dual ATBFs are implemented to the detection part of fluorescence lifetime imaging microscope (FLIM) system so that we obtained spectrally resolved FLIM images. We compare these SLIM images with an original FLIM image and confirm that the former case provides high accuracy to analyze lifetime distribution as well as high contrast of images. The proposed SLIM microscope with good wavelength selectivity has many opportunities to utilize to other applications such as FLIM-Föster resonant energy transfer and autofluorescence imaging.

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Section: Introductionmentioning

confidence: 99%

Spectrally resolved fluorescence lifetime imaging microscope using tunable bandpass filters

Jeong

Ahn

et al. 2012

Review of Scientific Instruments

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“…When the particle density is low, the effective scattering connected with optical feedback could not be attained. When the particle density is too high, the lifetime decay would be induced, resulting in reduction of gain [13]. The minimum lasing threshold was found to be 49 mW/pulse for a particle density of 2.0×10 13 cm −3 .…”

Section: Without Particlesmentioning

confidence: 97%

Threshold reduction induced by silica nanoparticledispersed active layer in diode-pumped microcavity dye laser

2009

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Abstract:We report a vertical microcavity dye laser consisting of a polymeric active random layer doped with silica nanoparticles whose diameters are approximately 100 nm. Pumped by an InGaN-based blue-violet laser diode with pulse duration of 3 ns, single mode laser oscillation was obtained at a wavelength of 530 nm. The threshold pump power decreased from 70 mW/pulse to 49 mW/pulse by adding nanoparticles to the active layer. We found an optimum particle density of 2×10 13 cm −3 by measuring threshold pump power to change depending on the particle density in the activity layer.

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“…Many experiments have been carried out with this purpose (e.g. [6][7][8][9][10][11][12][13][14][15][16][17]) whereby in this context, the research of O. War-burg is exemplary in terms of its scientific content and methodology. In 1936, O. Warburg developed the socalled "Optical Test", which uses the fluorescence of NADH + H + , the reduced form of NAD (nicotinamideadenine dinucleotide) [18].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the respiration of 3T3 cells by laser-induced fluorescence during a cyclic heating process

Beuthan¹,

Dressler²,

Zabaryło³

et al. 2010

Laser Phys. Lett.

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The use of lasers in the near infrared spectral range for laser-induced tumor therapy (LITT) demands a new understanding of the thermal responses to repetitive heat stress. The analysis of laser-induced fluorescence during vital monitoring offers an excellent opportunity to solve many of the related issues in this field. The laser-induced fluorescence of the cellular coenzyme NADH was investigated for its time and intensity behavior under heat stress conditions. Heat was applied to vital 3T3 cells (from 22• C to 50• C) according to a typical therapeutical time regime. A sharp increase in temperature resulted in non-linear time behavior when the concentration of this vital coenzyme changed. There are indications that biological systems have a delayed reaction on a cellular level. These results are therefore important for further dosimetric investigations.

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The study of structure and photophysical processes of polyelectrolyte-surfactant-dye molecules complex by laser spectroscopy

Abstract: Dependences of lifetime (curves 1,3 -polymerization degree 1510 links) and relevant association degree (curves 2,4 -PAA concentration 5 × 10 −4 M) on PAA concentration (curves 1,2) and polymerization degree (curves 3,4)

Cited by 7 publications

References 9 publications

Spectrally resolved fluorescence lifetime imaging microscope using tunable bandpass filters

Spectrally resolved fluorescence lifetime imaging microscope using tunable bandpass filters

Threshold reduction induced by silica nanoparticledispersed active layer in diode-pumped microcavity dye laser

Characterization of the respiration of 3T3 cells by laser-induced fluorescence during a cyclic heating process

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