Graphene oxide was prepared by the modified Hummers method and ultrasonic treatment, ammoniacal silver solution and glucose were added into graphene oxide suspension to prepare graphene oxide-silver (GO-Ag) nanoparticles. And then the achieved GO-Ag nanoparticles were incorporated into chitosan and graphene oxide to prepare graphene oxide-silver/graphene oxide /chitosan(GO-Ag/GO/CS) composites. The structure and properties of GO-Ag/GO/CS composite have been investigated by XRD, IR, TG, Tensile test, cytotoxicity test and antibacterial test. XRD and IR analysis indicated that GO-Ag was successfully compounded with graphene oxide and chitosan, the structure of GO-Ag was unchanged after being incorporation. Tensile test showed that the incorporation of graphene oxide into chitosan matrix can effectively improve the tensile strength of GO-Ag/GO/CS composite, especially the wet-state tensile strength. When incorporated of 4wt% GO into chitosan matrix, the wet-state tensile strengths of GO-Ag/GO/CS were improved by 214.3% compared with chitosan matrix. Thermogravimetric analysis indicated that the incorporation of graphene oxide has a slight effect on the thermal stability of CS. The results of cytotoxicity test showed that the cytotoxicity levels of GO-Ag/GO/CS composites were all in the range of non-cytotoxic when the dosages of GO-Ag were changing from 3.0 mg to 6.0 mg, which satisfied the safety standards of biomaterials. The bacteriostatic rate of GO-Ag/GO/CS-5 group (contain 5mg GO-Ag) against Staphylococcus aureus can reach 98.31%, showing excellent bacteriostatic effect, which could be used for further experimental study.