The submergence-induced gene OsCTP in rice (Oryza sativa L.) is similar to Escherichia coli cation transport protein ChaC

Qi, Yanhua; Yamauchi, Yasuo; Ling, Jianqun; Kawano, Naoyoshi; Li, Debao; Tanaka, Kiyoshi

doi:10.1016/j.plantsci.2004.07.004

Cited by 17 publications

(12 citation statements)

References 31 publications

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“…6). These results are similar to OsMGD and OsCTP gene expression at those conditions (Qi et al 2004(Qi et al , 2005. It also explained that the OsGGT gene might be associated with a general defensive response against various environmental stresses.…”

Section: Discussionsupporting

confidence: 69%

“…To further understand the physiological mechanisms of submergence and characterize a submergence-tolerant gene, we previously isolated several submergence-related genes, such as OsMGD (Oryza sativa monogalactosyldiacylglycerol synthase) and OsCTP (O. sativa cation transport protein) using a subtractive cDNA library from nonsubmerged and 7-day submerged rice plants of cultivar FR13A, which tolerates submergence (Qi et al 2004(Qi et al , 2005. Here, we report another new gene, OsGGT, from the subtractive cDNA library using suppression subtractive hybridization (SSH) and rapid amplification of cDNA ends (RACE).…”

Section: Introductionmentioning

confidence: 99%

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Identification and cloning of a submergence-induced gene OsGGT (glycogenin glucosyltransferase) from rice (Oryza sativa L.) by suppression subtractive hybridization

Kawano

Yamauchi

et al. 2005

Planta

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A submergence-induced gene, OsGGT, was cloned from 7-day submerged rice (Oryza sativa L. plants, FR13A (a submergence-tolerant cultivar, Indica), using suppression subtractive hybridization and both 5'- and 3'-rapid amplification of cDNA ends (RACE). The full-length OsGGT cDNA contains 1,273 bp with an open reading frame of 1,140 bp (17-1,156) that encodes 379 amino acids. Its deduced amino acid sequence is homologous with glycogenin glucosyltransferase. We found that the OsGGT gene is located in the 17,970-20,077 bp region of genome fragment AAAA01002475.1 of the Indica cultivar and in the 53,293-51,186 bp region of genome fragment AC037426.12 of chromosome 10 of the Japanica cultivar. A time-course study showed that OsGGT-gene expression increased in FR13A during submergence but decreased in IR42 (submergence-intolerant cultivar, Indica). The expression of the OsGGT gene in FR13A was induced by salicylic acid and benzyladenine. The accumulation of OsGGT mRNA in FR13A also increased in response to ethylene, gibberellin, abscisic acid, drought and salt treatment, but methyl jasmonate treatment and cold stress had no effect on expression. These results suggest that the OsGGT gene could be related to submergence stress and associated with a general defensive response to various environmental stresses.

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Section: Discussionsupporting

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Identification and cloning of a submergence-induced gene OsGGT (glycogenin glucosyltransferase) from rice (Oryza sativa L.) by suppression subtractive hybridization

Kawano

Yamauchi

et al. 2005

Planta

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“…V-PPase was Wrst reported to be induced in rice seedlings under anoxia (Carystinos et al 1995), and cDNA fragments encoding V-PPases were also isolated in a study on Xooding-induced genes in rice by a subtraction method (Agarwal and Grover 2005). A cation transporter-like protein is thought to be a homologue of OsCTP, which is induced by submergence in rice and structurally similar to cation transport protein ChaC in Escherichia coli (Qi et al 2005). This is the Wrst report to show that the expression of a gene encoding PIP is enhanced by anoxia.…”

Section: Isolation Of Genes Diverentially Expressed In Pondweed Turiomentioning

confidence: 99%

“…In the case of anoxic Arabidopsis, more than 600 out of over 22,500 genes were predicted by Loreti et al (2005) to be induced. From rice, the most tolerant plant species to anoxia among major crops, many genes up-regulated by Xooding stress have been isolated by subtractive hybridization methods (Agarwal and Grover 2005;Qi et al 2005;Sauter et al 2002). Caturla et al (2002) used suppression subtractive hybridization to isolate genes enhanced by submergence in adventitious root primordial of Sesbania rostrata.…”

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confidence: 99%

Anoxia-enhanced expression of genes isolated by suppression subtractive hybridization from pondweed (Potamogeton distinctus A. Benn.) turions

Harada

Satoh

Yoshioka

et al. 2007

Planta

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Pondweed (Potamogeton distinctus A. Benn.), a monocot aquatic plant species, has turions, which are overwintering buds forming underground as an asexual reproductive organ. Turions not only survive for more than one month but also elongate under strict anoxia, maintaining high-energy charge by activation of fermentation. We cloned 82 cDNA fragments of genes, that are up-regulated during anoxic growth of pondweed turions, by suppression subtractive hybridization. The transcript levels of 44 genes were confirmed to be higher under anoxia than those in air by both Northern blot analysis and a semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) method. A homology search for their nucleotide sequences revealed that some of them are highly homologous to known sequences of genes from other plants. They included alcohol dehydrogenase, pyruvate decarboxylase (PDC), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), vacuolar H(+)-translocating pyrophosphatase and a plasma membrane intrinsic protein. Time courses of transcript accumulation of some genes under anoxia were different from those in air. The activity of PDC increased under anoxic conditions but the activities of GAPDH and pyrophosphatase remained constant after anoxic treatment. Anoxically up-regulated genes are possibly involved in physiological events to control energy production, pH regulation and cell growth under anoxia. These results suggest that transcriptional regulation of these genes serves as an essential part of survival and growth of pondweed turions under anoxia.

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“…Paulose et al (2010) found a putative cation transporter associated protein (ChaC) in Crambe abyssinica cDNA. This protein has homology to the protein OsARP, which is highly expressed in rice under submergence, salt and drought stress (Qi et al, 2005) and stimulate salt tolerance when overexpressed in tobacco (Uddin et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Germination and initial growth of crambe (Crambe abyssinica Hochst.) under saline conditions

Pivetta¹,

Pivetta²,

Castoldi³

et al. 2016

Aust J Crop Sci

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Crambe is a promising crop for Brazil owing to its tolerance to drought and its short growing season (particularly if cultivated between seasons). However, the salinity tolerance of the unique Brazilian cultivar is not yet known. In this study, we aimed to determine the effects of different salinity levels on germination and initial growth of crambe cultivar FMS Brilhante. Therefore, an experiment was carried out in a completely randomized block design, during 19 days under greenhouse conditions. The treatments consisted of six levels of electrical conductivity of the soil saturation extract (EC): 2.50, 3.15, 3.35, 4.25, 5.75 and 9.55 dS m -1 , obtained by adding sodium chloride (NaCl) to soil. It was evaluated germination, germination speed index (GSI), length of roots and dry mass of shoot and roots. Overall, germination and GSI increased under low levels of salinity -up to 3.77 dS m -1 , caused by a possible break effect in seed dormancy. The initial growth was more sensitive to salinity than seed germination and the shoot dry matter decreased linearly with increasing salinity levels, indicating that FMS Brilhante cultivar is moderately sensitive to salinity.

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The submergence-induced gene OsCTP in rice (Oryza sativa L.) is similar to Escherichia coli cation transport protein ChaC

Cited by 17 publications

References 31 publications

Identification and cloning of a submergence-induced gene OsGGT (glycogenin glucosyltransferase) from rice (Oryza sativa L.) by suppression subtractive hybridization

Identification and cloning of a submergence-induced gene OsGGT (glycogenin glucosyltransferase) from rice (Oryza sativa L.) by suppression subtractive hybridization

Anoxia-enhanced expression of genes isolated by suppression subtractive hybridization from pondweed (Potamogeton distinctus A. Benn.) turions

Germination and initial growth of crambe (Crambe abyssinica Hochst.) under saline conditions

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