The sulphonylurea herbicide monosulphuron ester sodium as a special male gametocide in Brassica napus L.

Cheng, Yongqiang; Cui, Jianmin; Li, Z.-J.; Hu, Zhengqiang; Xing, Zheng; Wang, J.; Zhao, Huixian; Hu, Shen

doi:10.17221/233/2014-cjgpb

Cited by 5 publications

(5 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been confirmed that AHAS should be the target of AHAS-inhibiting herbicides ( Pang et al, 2003 ; Mccourt et al, 2005 , 2006 ). Recently, a few SU-herbicides were found to be excellent CHAs for rapeseed at a lower concentration ( Yu et al, 2006 , 2009 , 2017 ; Cheng et al, 2013 , 2015 ; Yu and He, 2014 ), which attracted many researchers to reveal its underlying mechanism using different approaches ( Cheng et al, 2013 ; Li Z. et al, 2015 ; Zhao et al, 2015 ; Liu et al, 2017 ). Transcriptional and proteomic analyses have identified many differential expression transcripts and proteins in monosulfuron-ester sodium- or amidosulfuron-induced male sterile rapeseed ( Cheng et al, 2013 ; Li Z. et al, 2015 ; Liu et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…Some chemicals have been reported as CHAs for hybrid seeds production in rapeseed ( Guan, 2014 ; Yu and He, 2014 ). A few of sulfonylurea herbicides, which are a class of the most widely used acetohydroxyacid synthase (AHAS, EC 2.2.1.6)-inhibiting herbicides, can induce complete male sterility in rapeseed at a concentration less than 1% of that required for their herbicide activity, such as tribenuron methyl (TBM) ( Yu et al, 2006 ; Zhang et al, 2010 ; Yu et al, 2017 ), monosulfuron-ester sodium ( Cheng et al, 2013 , 2015 ; Li Z. et al, 2015 ), and amidosulfuron ( Yu et al, 2009 ). So far, several dozens of commercial rapeseed ( B. napus ) hybrids based on male sterility induced by TBM or other sulfonylurea herbicides have been registered in China ( Cheng et al, 2013 ; Yu et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

“…It has been confirmed that AHAS is the target of AHAS-inhibiting herbicides ( Pang et al, 2003 ; Mccourt et al, 2005 , 2006 ). Recently, it was found that a few SU-herbicides can induce complete male sterility in B. napus at a lower concentration ( Yu et al, 2006 , 2009 , 2017 ; Cheng et al, 2013 , 2015 ), this suggested that these SU-herbicides are potential CHAs in rapeseed hybrid breeding. Previous studies tried to explore the mechanism underlying CIMS ( Cheng et al, 2013 ; Li Z. et al, 2015 ; Zhao et al, 2015 ; Liu et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Male Sterility of an AHAS-Mutant Induced by Tribenuron-Methyl Solution Correlated With the Decrease of AHAS Activity in Brassica napus L.

Huang

Sun

et al. 2018

Front. Plant Sci.

View full text Add to dashboard Cite

Tribenuron-methyl (TBM), an acetohydroxyacid synthase (AHAS)-inhibiting herbicide, can be used as an efficient chemical hybridization agent to induce male sterility for practical utilization of heterosis in rapeseed (Brassica napus L.). Utilization of rapeseed mutants harboring herbicide-resistant AHAS alleles as the male parent can simplify the hybrid seed production protocol. Here we characterized a novel TBM-resistant mutant K5 derived from an elite rapeseed variety, Zhongshuang No. 9 (ZS9), by ethyl methyl sulfonate mutagenesis. Comparative analysis of three BnAHAS genes (BnAHAS1, BnAHAS2, and BnAHAS3) between the mutant K5 and ZS9 identified a C-to-T transition at 544 from the translation start site in BnAHAS1 in K5 (This resistant allele is referred to as BnAHAS1544T), which resulted in a substitution of proline with serine at 182 in BnAHAS1. Both ZS9 and K5 plants could be induced complete male sterility under TBM treatment (with 0.10 and 20 mg⋅L-1 of TBM, respectively). The relationship between TBM-induced male sterility (Y) and the relative AHAS activity of inflorescences (X) could be described as a modified logistic function, Y = 100-A/(1+Be(-KX)) for the both genotypes, although the obtained constants A, B, and K were different in the functions of ZS9 and K5. Transgenic Arabidopsis plants expressing BnAHAS1544T exhibited a higher TBM resistance of male reproductive organ than wild type, which confirmed that the Pro-182-Ser substitution in BnAHAS1 was responsible for higher TBM-resistance of male reproductive organs. Taken together, our findings provide a novel valuable rapeseed mutant for hybrid breeding by chemical hybridization agents and support the hypothesis that AHAS should be the target of the AHAS-inhibiting herbicide TBM when it is used as chemical hybridization agent in rapeseed.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Male Sterility of an AHAS-Mutant Induced by Tribenuron-Methyl Solution Correlated With the Decrease of AHAS Activity in Brassica napus L.

Huang

Sun

et al. 2018

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Selective induction of male sterility using chemical hybridizing agents (CHAs) was first demonstrated in the mid‐20th century (Moore, 1950; Naylor, 1950). To date, numerous chemicals have been evaluated for their applicability as CHAs in different crop species (Chakraborty & Devakumar, 2006a; Kajal Chakraborty & C Devakumar, 2006; Zhang et al., 2017) and some crop specific chemicals are also identified for cotton ( Gossypium hirsutum L.) and Brassica s (Cheng et al., 2015; Ladyman et al., 1990). Unfortunately, most of the evaluated CHAs reduce female fertility, and show varying levels of phytotoxicity ranging from plant growth inhibition and/or reduced seed set (McRae, 1985; Sharma & Sharma, 2005).…”

Section: Introductionmentioning

confidence: 99%

Analysis of effects of trifluoromethanesulfonamide in sorghum inbred lines and their derived hybrids

2021

View full text Add to dashboard Cite

In sorghum [Sorghum bicolor (L.) Moench], development of male‐sterile versions (A‐lines) of the seed parents (B‐lines) is a tedious and time‐consuming process limiting opportunities for the improvement of new seed parents. Assessment of the value of a potential new seed parent requires testcross hybrid evaluations, but because of male fertility of these lines, conventional methods are inadequate to generate hybrid seed required for testing. Mechanisms to reduce the number of lines that require sterilization would be beneficial. The chemical gametocide trifluoromethanesulfonamide (TFMSA) was evaluated for its potential applicability to generate testcross hybrid seed. To study the effect on inbred line productivity, foliar applications of 25 mg of TFMSA were made to a select set of male‐fertile B‐lines and were compared to the corresponding untreated male‐sterile A‐line. Trifluoromethanesulfonamide induced near‐complete male‐sterility and allowed for testcross hybrid seed production across a range of genotypes. Comparison to untreated B‐lines indicated an average 3‐d delay in flowering and a 13‐cm reduction in plant height. Furthermore, average seed weight per panicle (productivity) was reduced by 8 g. Comparison of testcross hybrids produced by TFMSA induced male‐sterile lines and cytoplasmic male‐sterile lines did not reveal any significant and meaningful differences between the hybrids derived from both sterility systems. Moreover, inbred lines produced hybrids that ranked consistently in both systems. These results show TFMSA can affect productivity of treated inbred lines, but not the derived hybrid, which demonstrates that TFMSA can be used to produce hybrids for evaluation prior to investment in sterilizing of these lines.

show abstract

“…AHAS activity requires three cofactors: thiamine diphosphate (ThDP), magnesium ion (Mg 2+ ), and flavin adenine dinucleotide (FAD). Recently, Chinese scientists have demonstrated that some AHAS inhibitors, such as tribenuron-methyl (TBM), amidosulfuron, monosulfuron ester sodium (MES), and SX-1 could act as effective male gametocides when applied at very low doses in B. napus ( Cheng et al 2013 , 2015 , Li et al 2015 , Ning et al 2018 , Yu et al 2006 , 2009 ). Furthermore, male sterility induced by SX-1 in B. napus was analyzed using a multi-omics integrative analysis, and the initial deficiency of protein processing in the endoplasmic reticulum and flavonoid biosynthesis were proposed as the main reasons for its effectiveness ( Ning et al 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of tribenuron-methyl-induced male sterility in <i>Brassica juncea</i> L.

Guo

Gao

et al. 2021

Breed. Sci.

View full text Add to dashboard Cite

Significant heterosis has been documented in Brassica juncea L. that are grown as agriculturally important oilseeds, vegetables and condiments crops. Male sterility induced by chemical hybridizing agents is an important pollination control system in hybrid crop breeding. Herein, we show that tribenuron-methyl (TBM), a sulfonylurea herbicide, is an effective male gametocide in B. juncea when used at a very low dosage. In the present study, foliar application of various rates of TBM induced a significant increase in pollen sterility in B. juncea (90.57-100%). TBM-treated plants exhibited reductions in size of floral organ and yield components; however, lower dose of TBM (0.075 g a.i. ha -1 ) did not cause a significant reduction in seed yield per plant. Tapetum cells of TBM-treated plants were hypertrophied and degenerated earlier, and abnormal meiosis was observed at the meiotic stage. A significant decrease of acetohydroxyacid synthase (AHAS) activities was detected in buds of plants treated with 0.10 g a.i. ha -1 TBM, and RT-qPCR analysis showed that TBM exposure perturbed AHAS expression in small buds, which support that TBM induces male sterility in B. juncea by targeting AHAS expression. Our results suggest that TBM could be used as an efficient chemical hybridization agent in B. juncea, which has practical implications for the application of hybrid breeding in B. juncea.

show abstract

The sulphonylurea herbicide monosulphuron ester sodium as a special male gametocide in Brassica napus L.

Cited by 5 publications

References 6 publications

Male Sterility of an AHAS-Mutant Induced by Tribenuron-Methyl Solution Correlated With the Decrease of AHAS Activity in Brassica napus L.

Male Sterility of an AHAS-Mutant Induced by Tribenuron-Methyl Solution Correlated With the Decrease of AHAS Activity in Brassica napus L.

Analysis of effects of trifluoromethanesulfonamide in sorghum inbred lines and their derived hybrids

Characterization of tribenuron-methyl-induced male sterility in <i>Brassica juncea</i> L.

Contact Info

Product

Resources

About