The supplementation of Rothia as a potential preventive approach for bone loss in mice with ovariectomy‐induced osteoporosis

Abstract

There is an inseparable link between bone metabolism and gut microbiota, and the supplementation of probiotics exhibits a significant role in maintaining the homeostasis of gut microbiota and inhibiting bone loss. This study aims to explore the preventive and therapeutic potentials and the specific mechanisms of Rothia on osteoporosis. The mice models of osteoporosis induced by ovariectomy (OVX) were built, and the regular (once a day) and quantitative (200 μL/d) gavage of Rothia was performed for 8 weeks starting from 1 week after OVX. Microcomputed tomography was used to analyze the bone mass and bone microstructure of mice in each group after sacrifice. Histological staining and immunohistochemistry were then applied to identify the expression of pro‐inflammatory cytokines, intestinal permeability, and osteogenic and osteoclastic activities of mice. The collected feces of mice in each group were used for 16S rRNA high‐throughput sequencing to detect the alterations in composition, abundance, and diversity of gut microbiota. This study demonstrated that the gavage of Rothia alleviated bone loss in mice with OVX‐induced osteoporosis, improved OVX‐induced intestinal mucosal barrier injury, optimized intestinal permeability (zonula occludens protein 1 and occludin), reduced intestinal inflammation (tumor necrosis factor‐α and interleukin‐1β), and regulated imbalance of gut microbiota. Based on “gut‐bone” axis, this study revealed that regular and quantitative gavage of Rothia can relieve bone loss in mice with OVX‐induced osteoporosis by repairing the intestinal mucosal barrier injury, optimizing the intestinal permeability, inhibiting the release of pro‐inflammatory cytokines, and improving the disorder of gut microbiota.

Mapping knowledge landscapes and research frontiers of gastrointestinal microbiota and bone metabolism: a text-mining study

“…To further quantify the frequency distribution of these keywords, the study summarized the top 20 high-frequency keywords in Table 2 . Apart from the search term gut microbiota, other popular keywords in this research field include osteoporosis ( Biver et al., 2019 ; Xu et al., 2022 ; Lyu et al., 2023 ), bone density ( Wang et al., 2023 ; Zhang et al., 2023b ), probiotics ( Jia et al., 2021 ), inflammation ( Ding et al., 2022 ; Zhu et al., 2022 ), SCFAs ( Derrien et al., 2004 ; Psichas et al., 2015 ; Whisner et al., 2016 ; Larraufie et al., 2018 ; Nath et al., 2018 ; Shetty et al., 2018 ; Keirns et al., 2020 ; Massy and Drueke, 2020 ; Mohamed et al., 2024 ; Verstraeten et al., 2024 ), metabolism ( Zhang et al., 2023a ), osteoarthritis ( Liu et al., 2022 ; Guan et al., 2023 ), calcium absorption ( Zhou et al., 2023 ), obesity ( Sato et al., 2021 ), double-blind ( Nilsson et al., 2018 ; Jansson et al., 2019 ), prebiotics ( Mohamed et al., 2024 ), mechanisms ( Zhou et al., 2023 ), postmenopausal women ( Wang et al., 2023 ), supplements ( Li et al., 2023 ), risk factors ( Chen et al., 2021 ), oxidative stress ( Li et al., 2019 ), and immune system ( Kim et al., 2017 ; Dar et al., 2018b ; Dar et al., 2018a ). Similarly to citation burst analysis, this study also conducted a burst analysis on keywords that garnered significant attention during a certain period from 2000 to 2023.…”

Linking the relationship between dietary folic acid intake and risk of osteoporosis among middle‐aged and older people: A nationwide population‐based study

“…Additionally, a healthy, reasonable, balanced, and nutritious diet is the foundation for maintaining the bone and whole health of middle‐aged and older people (Farshbaf‐Khalili et al., 2023 ; Kheiridoost et al., 2022 ; Li et al., 2024 ), and the prevention and treatment of osteoporosis also requires cooperative management from multiple perspectives (Warensjö et al., 2011 ; Zhang, Cao, Li, Dai, Lu, Zhang, Bai, Chen, Shi, et al., 2022 ). In addition to actively seeking medical treatment, it is necessary to pay attention to rational diets in daily life to supplement the necessary nutrients for the bones (Zhang, Lu, et al., 2021 ).…”