The synergetic effect of bioactive ceramic and nanoclay on the properties of chitosan–gelatin/nanohydroxyapatite–montmorillonite scaffold for bone tissue engineering

Olad, Ali; Azhar, Fahimeh Farshi

doi:10.1016/j.ceramint.2014.04.010

Cited by 128 publications

(51 citation statements)

References 65 publications

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“…Figure presents the XRD pattern for Lap, chitosan scaffold and nanocomposite scaffolds at different Lap loadings. The diffraction pattern for chitosan scaffold presents peaks on ~2θ = 10.1 and 20°, which corresponds to hydrated crystalline structure and to an amorphous structure of chitosan, respectively (Gabriel, Gonzaga, Poli, & Schmitt, ; Olad & Farshi Azhar, ).…”

Section: Resultsmentioning

confidence: 99%

“…The addition of 5%wt of Laponite increased the average pore size and porosity. It is worth mentioning, in the literature is reported that scaffolds should have porosity above 50% to be applied as dressing (Elsner, Kraitzer, Grinberg, & Zilberman, ; Govindan et al, ; Olad & Farshi Azhar, ). Pure chitosan scaffold did not reach minimum porosity value for application as a wound dressing.…”

Section: Discussionmentioning

confidence: 99%

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Chitosan‐laponite nanocomposite scaffolds for wound dressing application

et al. 2019

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The pivotal issue of skin regeneration research is the development of effective biomaterials that exhibit biological activities as fungicide and bactericide, combining simple and low cost manufacturing technologies. In this context, nanocomposite scaffolds based on chitosan (Ch)/Laponite (Lap) were produced by using different concentrations of Lap via freeze‐drying process for potential application in skin regeneration. The influence of Lap concentration on the scaffold properties was evaluated. The prepared scaffolds were characterized by x‐ray diffraction (XRD), scanning electron microscopy (SEM), porosity, swelling capacity, and mechanical analyses. The results revealed that the scaffolds exhibited a porous architecture, besides the increase in the clay content, leads to an increase in the porosity, an improvement of mechanical strength, and a decrease of swelling capacity. In vitro tests were also carried out to evaluate the biocompatibility of the materials, such as bioadhesion, antibacterial activity, viability, and cell adhesion. Viability and cell adhesion demonstrated that all scaffolds were not cytotoxic and the fibroblast cells readily attached on the surface of the scaffolds. Thereby, the results suggested that the nanocomposite scaffolds are biomaterials potentially useful as wound dressings.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Chitosan‐laponite nanocomposite scaffolds for wound dressing application

et al. 2019

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“…CHI-gelatin/MMT-HA scaffolds were prepared through freeze drying showing a nanoscale architecture with well-interconnected pores, with a mean pore size of 250 μm, similar to natural bone. [121] Sun and coworkers [122] synthetized multilayered CHI/CNTs nanocomposite films, through freeze drying and solvent casting, and found that the tensile stress and ductility of solvent casted films are higher than by freeze drying, probably due to the porosity. [123]…”

Section: Materials Processing Strategies For Chitosan Nanocompositesmentioning

confidence: 99%

Chitosan nanocomposites based on distinct inorganic fillers for biomedical applications

Moura

Mano

Paiva

et al. 2016

Science and Technology of Advanced Materials

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Chitosan (CHI), a biocompatible and biodegradable polysaccharide with the ability to provide a non-protein matrix for tissue growth, is considered to be an ideal material in the biomedical field. However, the lack of good mechanical properties limits its applications. In order to overcome this drawback, CHI has been combined with different polymers and fillers, leading to a variety of chitosan-based nanocomposites. The extensive research on CHI nanocomposites as well as their main biomedical applications are reviewed in this paper. An overview of the different fillers and assembly techniques available to produce CHI nanocomposites is presented. Finally, the properties of such nanocomposites are discussed with particular focus on bone regeneration, drug delivery, wound healing and biosensing applications.

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“…However, collagen possesses antigenicity because it is extracted from animal origin. Gelatin is a hydrolysate product of collagen and, thus, has a relatively lower antigenicity than collagen, and gelatin also has a low cost . In addition, gelatin also retains some information signals, such as arginine–glycine–aspartic acid (RGD), which is conducive to the acceleration of cell differentiation, proliferation, and attachment to materials.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, gelatin also retains some information signals, such as arginine–glycine–aspartic acid (RGD), which is conducive to the acceleration of cell differentiation, proliferation, and attachment to materials. In the study of the effects of the products and production costs, the combination of chitosan and gelatin has been used commonly in bone tissue engineering . Both chitosan and gelatin are biodegradable, biocompatible, and nontoxic, and they are beneficial for cell attachment and proliferation.…”

Section: Introductionmentioning

confidence: 99%

Chitosan/gelatin porous bone scaffolds made by crosslinking treatment and freeze‐drying technology: Effects of crosslinking durations on the porous structure, compressive strength, and in vitro cytotoxicity

Lou

Wen

Lin

2015

J of Applied Polymer Sci

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In this study, freezing was used to separate a solute (polymer) and solvent (deionized water). The polymer in the ice crystals was then crosslinked with solvents, and this diminished the linear pores to form a porous structure. Gelatin and chitosan were blended and frozen, after which crosslinking agents were added, and the whole was frozen again and then freeze-dried to form chitosan/gelatin porous bone scaffolds. Stereomicroscopy, scanning electron microscopy, compressive strength testing, porosity testing, in vitro biocompatibility, and cytotoxicity were used to evaluate the properties of the bone scaffolds. The test results show that both crosslinking agents, glutaraldehyde (GA) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, were able to form a porous structure. In addition, the compressive strength increased as a result of the increased crosslinking time. However, the porosity and cell viability were not correlated with the crosslinking times. The optimal porous and interconnected pore structure occurred when the bone scaffolds were crosslinked with GA for 20 min. It was proven that crosslinking the frozen polymers successfully resulted in a division of the linear pores, and this resulted in interconnected multiple pores and a compressively strong structure. The 48-h cytotoxicity did not affect the cell viability. This study successfully produced chitosan/gelatin porous materials for biomaterials application.

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The synergetic effect of bioactive ceramic and nanoclay on the properties of chitosan–gelatin/nanohydroxyapatite–montmorillonite scaffold for bone tissue engineering

Cited by 128 publications

References 65 publications

Chitosan‐laponite nanocomposite scaffolds for wound dressing application

Chitosan‐laponite nanocomposite scaffolds for wound dressing application

Chitosan nanocomposites based on distinct inorganic fillers for biomedical applications

Chitosan/gelatin porous bone scaffolds made by crosslinking treatment and freeze‐drying technology: Effects of crosslinking durations on the porous structure, compressive strength, and in vitro cytotoxicity

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