The synergistic effect on osteogenic differentiation of human mesenchymal stem cells by diode laser-treated stimulating human umbilical vein endothelial cells

Abstract

Angiogenesis plays an important role in determining the biostimulation of bone regeneration, in either new bone or blood vessel formation. Human umbilical cord cells (HUVECs) are important effector cells in angiogenesis and are indispensable for osteogenesis and for their heterogeneity and plasticity. However, there are very few studies about the effects of HUVECs on diode laser-stimulated/regulated osteogenesis. In this study, we used diode laser as a model biostimulation to examine the role of HUVECs on laser-stimulated osteogenesis. Several bone formation-related proteins were also significantly up-regulated by the diode laser stimulation, indicating that HUVECs may participate in diode laser-stimulated osteogenesis. Interestingly, when human mesenchymal stem cells (hMSCs) cultured with HUVECs were diode laser-treated, the osteogenesis differentiation of the hMSCs was significantly promoted, indicating the important role of HUVECs in diode laser-enhanced osteogenesis. Adequately activated HUVECs are vital for the success of diode laser-stimulated hard-tissue regeneration. These findings provided valuable insights into the mechanism of diode laser-stimulated osteogenic differentiation, and a strategy to optimize the evaluation system for the in vitro osteogenesis capacity of laser treatment in periodontal repair.

Exploring the cell interactome: deciphering relative impacts of cell–cell communication in cell co-culture using a novel microfluidic device

“…Alkaline phosphatase (ALP) is well established in the literature as an early marker for osteogenesis, 22,36,37 and was thus chosen as the most appropriate method for assessing the osteogenesis of MSCs. Using traditional co-culture methods, the complexity of cell–cell interactions is apparent.…”

Synergies of Human Umbilical Vein Endothelial Cell-Laden Calcium Silicate-Activated Gelatin Methacrylate for Accelerating 3D Human Dental Pulp Stem Cell Differentiation for Endodontic Regeneration

“…In the past, many studies have confirmed that the cytokines released during angiogenesis can effectively promote the differentiation of surrounding cells [ 51 ]. However, the crosstalk between hDPSCs and HUVEC can direct the differentiation of stem cells toward stem cells that later wrap osteo-/odontogenesis to form functional hard tissue [ 52 ].…”

“…Analysis of human tissues show that endothelial cells in normal surrounding tissue express OPG. Moreover, HUVEC and other endothelial cells both expressed OPG during angiogenesis [ 51 ]. Therefore, the above results not only indicate that Si plays a role in enhancing angiogenesis, but also stimulate HUVEC to secrete a variety of cytokines that can assist odontogenic differentiation, which is beneficial to endodontic regeneration engineering.…”

“…Hence, we can conclude that Si-containing FGelMa hydrogel was one of the key components in supporting the cell function of HUVEC and was able to produce a synergistic effect in odontogenesis for regeneration. In the past, many studies have confirmed the cytokines released during angiogenesis can effectively promote the differentiation of surrounding cells [51]. However, the crosstalk between hDPSCs and HUVEC can direct the differentiation of stem cells toward stem cells that later wrap osteo-/odontogenesis to form functional hard tissue [52].…”

3D-Printed Bioactive Calcium Silicate/Poly-ε-Caprolactone Bioscaffolds Modified with Biomimetic Extracellular Matrices for Bone Regeneration

“…When compared to their correlating control groups, DPCL and DCS increased cellular proliferation by 1.31- and 1.43-times after seven days of cell culturing, respectively. After cell adhesion and proliferation, cells can not only secrete even more ECM proteins, such as FN and Col I, but can also respond to various ECM components that help to maintain homeostasis [ 43 ]. In general, enhanced interactions between cells and ECM result in better cellular adhesion, which could trigger cell signaling cascades that up-regulate the phosphorylation of several proteins, such as focal adhesion kinase [ 34 ], providing an synergistic effect on cellular proliferation.…”